Recombinant Dna Methodology Ii
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Recombinant Dna Methodology Ii
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Author : Ray Wu
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Recombinant Dna Methodology Ii written by Ray Wu and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.* Methods for: * DNA isolation and cloning* Synthesizing complementary DNA (cDNA)* Cleaving and manipulating DNA * Selecting useful reporter genes* Constructing vectors for cloning genes* Constructing expression vectors* Site-directed mutagenesis and gene disruption* Identifying and mapping genes* Transforming animal and plant cells* Sequencing DNA* Amplifying and manipulating DNA and PCR* Detecting DNA - protein interaction
Recombinant Dna Methodology
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Author :
language : en
Publisher: Academic Press
Release Date : 2014-05-19
Recombinant Dna Methodology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-19 with Science categories.
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. - Enzymes in DNA research - DNA isolation, hybridization, and cloning - DNA sequence analysis - cDNA cloning - Gene products - Identification of cloned genes and mapping of genes - Monitoring cloned gene expression - Cloning and transferring of genes into yeast cells - Cloning and transferring of genes into plant cells - Cloning and transferring of genes into animal cells - Site-directed mutagenesis - Protein engineering - Expression vectors
Recombinant Dna Technology
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Author : Siddra Ijaz
language : en
Publisher: Cambridge Scholars Publishing
Release Date : 2019-08-13
Recombinant Dna Technology written by Siddra Ijaz and has been published by Cambridge Scholars Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Science categories.
Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Manipulation And Expression Of Recombinant Dna
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Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Recombinant Dna Laboratory Manual
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Author : Judith W. Zyskind
language : en
Publisher: Elsevier
Release Date : 2014-05-12
Recombinant Dna Laboratory Manual written by Judith W. Zyskind and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Science categories.
Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Recombinant Dna Principles And Methodologies
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Author : James Greene
language : en
Publisher: CRC Press
Release Date : 1998-06-25
Recombinant Dna Principles And Methodologies written by James Greene and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-06-25 with Science categories.
This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
Recombinant Dna Technology
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Author : Keya Chaudhuri
language : en
Publisher: The Energy and Resources Institute (TERI)
Release Date : 2013-01-01
Recombinant Dna Technology written by Keya Chaudhuri and has been published by The Energy and Resources Institute (TERI) this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-01 with Science categories.
Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cells categories.
Fundamentals Of Food Biotechnology
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Author : Byong H. Lee
language : en
Publisher: John Wiley & Sons
Release Date : 2015-02-16
Fundamentals Of Food Biotechnology written by Byong H. Lee and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-02-16 with Science categories.
Fundamentals of Food Biotechnology Food biotechnology is the application of modern biotechnological techniques to the manufacture and processing of food; for example, through fermentation of food (which is the oldest biotechnological process) and food additives, as well as plant and animal cell cultures. New developments in fermentation and enzyme technological processes, molecular thermodynamics, genetic engineering, protein engineering, metabolic engineering, bioengineering, and processes involving monoclonal antibodies, nanobiotechnology and quorum sensing have introduced exciting new dimensions to food biotechnology, a burgeoning field that transcends many scientific disciplines. Fundamentals of Food Biotechnology, 2nd edition is based on the author’s 25 years of experience in teaching on a food biotechnology course at McGill University in Canada. The book will appeal to professional food scientists as well as graduate and advanced undergraduate students by addressing the latest exciting food biotechnology research in areas such as genetically modified foods (GMOs), bioenergy, bioplastics, functional foods/ nutraceuticals, nanobiotechnology, quorum sensing and quenching. In addition, cloning techniques for bacterial and yeast enzymes are included in a “New Trends and Tools” section and selected references, questions, and answers appear at the end of each chapter. This new edition has been comprehensively rewritten and restructured to reflect the new technologies, products, and trends that have emerged since the original book. Many new aspects highlight the short- and longer-term commercial potential of food biotechnology. Food Biochemistry and Food Processing, 2nd Edition Edited by Benjamin K. Simpson, Leo M.L. Nollet, Fidel Toldra, et al. ISBN 978-0-8138-0874-1 Food Processing: Principles and Applications, 2nd Edition Edited by Stephanie Clark (Editor), Stephanie Jung, Buddhi Lamsal ISBN 978-0-470-67114-6
Recombinant Dna
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Author : James D. Watson
language : en
Publisher: Macmillan
Release Date : 1992-02-15
Recombinant Dna written by James D. Watson and has been published by Macmillan this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-02-15 with Medical categories.
An overview of recombitant DNA techniques and surveys advances in recombinant molecular genetics, experimental methods and their results.