Recombinant Dna Methodology Ii
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Recombinant Dna Methodology
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Author :
language : en
Publisher: Academic Press
Release Date : 2014-05-19
Recombinant Dna Methodology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-19 with Science categories.
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors
Recombinant Dna Methodology Ii
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Author : Ray Wu
language : en
Publisher:
Release Date : 1995
Recombinant Dna Methodology Ii written by Ray Wu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Enzymology Primer For Recombinant Dna Technology
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Author : Hyone-Myong Eun
language : en
Publisher: Elsevier
Release Date : 1996-06-03
Enzymology Primer For Recombinant Dna Technology written by Hyone-Myong Eun and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-03 with Science categories.
Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Recombinant Dna Technology
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Author : Siddra Ijaz
language : en
Publisher: Cambridge Scholars Publishing
Release Date : 2019-08-13
Recombinant Dna Technology written by Siddra Ijaz and has been published by Cambridge Scholars Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Science categories.
Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Recombinant Dna Methodology
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Author : Jo-Anne R. Dillon
language : en
Publisher: New York : Wiley
Release Date : 1985-01-21
Recombinant Dna Methodology written by Jo-Anne R. Dillon and has been published by New York : Wiley this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-01-21 with Science categories.
Basic techniques; Gene cloning in Saccharomyces cerevisiae; Gene cloning in Pseudomonas aeruginosa; Mutant construction by in vitro mutagenesis.
Recombinant Dna Techniques
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Author : Raymond L. Rodriguez
language : en
Publisher: Addison Wesley Publishing Company
Release Date : 1983
Recombinant Dna Techniques written by Raymond L. Rodriguez and has been published by Addison Wesley Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Genetic engineering categories.
Recombinant Dna Principles And Methodologies
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Author : James Greene
language : en
Publisher: CRC Press
Release Date : 2021-12-17
Recombinant Dna Principles And Methodologies written by James Greene and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-17 with Science categories.
This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
Manipulation And Expression Of Recombinant Dna
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Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Recombinant Dna Laboratory Manual
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Author : Judith W. Zyskind
language : en
Publisher: Elsevier
Release Date : 2014-05-12
Recombinant Dna Laboratory Manual written by Judith W. Zyskind and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Science categories.
Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Recombinant Dna Technology Ii
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Author : Rakesh K. Bajpai
language : en
Publisher:
Release Date : 1994
Recombinant Dna Technology Ii written by Rakesh K. Bajpai and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.