Recombinant Dna Laboratory Manual
DOWNLOAD
Download Recombinant Dna Laboratory Manual PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Recombinant Dna Laboratory Manual book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Recombinant Dna Laboratory Manual Revised Edition
DOWNLOAD
Author : Judith W. Zyskind
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Recombinant Dna Laboratory Manual Revised Edition written by Judith W. Zyskind and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. - Comb-bound, three-column, large 9-1/4" x 7-1/2" format - Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts - Necessary reagents and equipment are presented in a checklist at the beginning of each protocol - Techniques for bacteria are complemented with those for Drosophila - Each experiment has been tested in the laboratory by students for five years - Features a complete chapter on computers in the molecular biology laboratory - Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains
Recombinant Dna Laboratory Manual
DOWNLOAD
Author : Judith W. Zyskind
language : en
Publisher: Morgan Kaufmann
Release Date : 1999
Recombinant Dna Laboratory Manual written by Judith W. Zyskind and has been published by Morgan Kaufmann this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Science categories.
Molecular Biology Techniques
DOWNLOAD
Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Laboratory Manual For Genetic Engineering
DOWNLOAD
Author : VENNISON, S. JOHN
language : en
Publisher: PHI Learning Pvt. Ltd.
Release Date : 2009-01-01
Laboratory Manual For Genetic Engineering written by VENNISON, S. JOHN and has been published by PHI Learning Pvt. Ltd. this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Science categories.
This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.
Recombinant Dna And Biotechnology
DOWNLOAD
Author : Helen Kreuzer
language : en
Publisher:
Release Date : 2001
Recombinant Dna And Biotechnology written by Helen Kreuzer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Biotechnology categories.
: Written in clear, easy–to–understand language, this best–selling reference text and activities manual offers easy–to–implement lessons and classroom activities. Part I covers basic molecular biology, and Part II offers imaginative dry labs and wet labs that can be done by both college and precollege students. Part III is an innovative section addressing the social issues and public concerns of biotechnology. Extensive appendixes provide important background information on basic laboratory techniques and teaching resources, including overhead masters and templates. Adopted by numerous school systems, this unique book is an outgrowth of molecular biology and biotechnology teaching workshops. All of the exercises and lab activities have been extensively tested in the classroom by hundreds of high school teachers. Recombinant DNA and Biotechnology is designed to interest an international teaching audience and will enable all instructors to teach a reasonable amount of molecular biology and genetic engineering to students. No other book makes it so easy or compelling for teachers to incorporate the "new biology" into their biology, biological sciences, or general science curriculum. Recombinant DNA and Biotechnology: A Guide for Teachers will enable college and precollege teachers to plan and conduct an exciting and contemporary course on the basic principles, essential laboratory activities, and relevant social issues and concerns attendant to today′s molecular biology revolution. In addition to the complete text of the student edition, A Guide for Teachers also contains the answers to all discussion questions and extra background information and material on the scientific principles involved.
Manipulation And Expression Of Recombinant Dna
DOWNLOAD
Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Recombinant Dna Methodology
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2014-05-19
Recombinant Dna Methodology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-19 with Science categories.
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. - Enzymes in DNA research - DNA isolation, hybridization, and cloning - DNA sequence analysis - cDNA cloning - Gene products - Identification of cloned genes and mapping of genes - Monitoring cloned gene expression - Cloning and transferring of genes into yeast cells - Cloning and transferring of genes into plant cells - Cloning and transferring of genes into animal cells - Site-directed mutagenesis - Protein engineering - Expression vectors
Molecular Neuroscience
DOWNLOAD
Author : Rusty Lansford
language : en
Publisher:
Release Date : 2014
Molecular Neuroscience written by Rusty Lansford and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Genes categories.
A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.
Basic Techniques In Molecular Biology
DOWNLOAD
Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Genetics
DOWNLOAD
Author : Gregore Koliantz
language : en
Publisher:
Release Date : 2009
Genetics written by Gregore Koliantz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Electronic books categories.
Cell Division(Mitosis): Looking at Chromosomes -- Restriction Testing -- Extraction of Genomic DNA -- Polymerase Chain Reaction -- Cloning 1 Generating Recombinant Plasmids -- Cloning 2 Minipreping -- Southern Blotting 1 DNA Transfer -- Southern Blotting 2 DNA-DNA Hybridization and Sequence Detection -- Regulation of Gene Expression -- Physical Properties of DNA and DNA Assay -- Transmission Genetics (Heredity) -- Meiosis and Analysis of Crossing Over -- Complementation Test -- Molecular Markers: Mapping the Genome of Arabidopsis -- Population Genetics How Changes Occur within a Population -- Front Matter.