Recombinant Dna Laboratory Manual Revised Edition
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Recombinant Dna Laboratory Manual Revised Edition
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Author : Judith W. Zyskind
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Recombinant Dna Laboratory Manual Revised Edition written by Judith W. Zyskind and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. - Comb-bound, three-column, large 9-1/4" x 7-1/2" format - Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts - Necessary reagents and equipment are presented in a checklist at the beginning of each protocol - Techniques for bacteria are complemented with those for Drosophila - Each experiment has been tested in the laboratory by students for five years - Features a complete chapter on computers in the molecular biology laboratory - Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains
Recombinant Dna Laboratory Manual
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Author : Judith W. Zyskind
language : en
Publisher: Elsevier
Release Date : 2014-05-12
Recombinant Dna Laboratory Manual written by Judith W. Zyskind and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Science categories.
Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Manipulation And Expression Of Recombinant Dna
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Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Recombinant Dna And Biotechnology
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Author : Helen Kreuzer
language : en
Publisher:
Release Date : 2001
Recombinant Dna And Biotechnology written by Helen Kreuzer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Biotechnology categories.
: Written in clear, easy–to–understand language, this best–selling reference text and activities manual offers easy–to–implement lessons and classroom activities. Part I covers basic molecular biology, and Part II offers imaginative dry labs and wet labs that can be done by both college and precollege students. Part III is an innovative section addressing the social issues and public concerns of biotechnology. Extensive appendixes provide important background information on basic laboratory techniques and teaching resources, including overhead masters and templates. Adopted by numerous school systems, this unique book is an outgrowth of molecular biology and biotechnology teaching workshops. All of the exercises and lab activities have been extensively tested in the classroom by hundreds of high school teachers. Recombinant DNA and Biotechnology is designed to interest an international teaching audience and will enable all instructors to teach a reasonable amount of molecular biology and genetic engineering to students. No other book makes it so easy or compelling for teachers to incorporate the "new biology" into their biology, biological sciences, or general science curriculum. Recombinant DNA and Biotechnology: A Guide for Teachers will enable college and precollege teachers to plan and conduct an exciting and contemporary course on the basic principles, essential laboratory activities, and relevant social issues and concerns attendant to today′s molecular biology revolution. In addition to the complete text of the student edition, A Guide for Teachers also contains the answers to all discussion questions and extra background information and material on the scientific principles involved.
Asm News
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Author :
language : en
Publisher:
Release Date : 2002
Asm News written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Microbiology categories.
Molecular Cloning
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Author : Joseph Sambrook
language : en
Publisher:
Release Date : 2003
Molecular Cloning written by Joseph Sambrook and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Eukaryotic cells categories.
Current Catalog
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Author : National Library of Medicine (U.S.)
language : en
Publisher:
Release Date : 1985
Current Catalog written by National Library of Medicine (U.S.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Medicine categories.
First multi-year cumulation covers six years: 1965-70.
Fundamental Laboratory Approaches For Biochemistry And Biotechnology
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Author : Alexander J. Ninfa
language : en
Publisher: John Wiley & Sons
Release Date : 2009-05-26
Fundamental Laboratory Approaches For Biochemistry And Biotechnology written by Alexander J. Ninfa and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-05-26 with Science categories.
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills, allowing students to learn techniques and develop the the critical thinking and organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques but also includes molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The experiments are designed so that theory and technique are learned as fundamental research tools, and the biochemistry and molecular biology applications are seamlessly integrated throughout the manual. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors’ three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual’s experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a “cookbook” approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Human Genome News
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Author :
language : en
Publisher:
Release Date : 1989
Human Genome News written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Human genome categories.