Regulators And Effectors Of Small Gtpases Part E Gtpases Involved In Vesicular Traffic
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Lipidomics And Bioactive Lipids Specialized Analytical Methods And Lipids In Disease
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-11-09
Lipidomics And Bioactive Lipids Specialized Analytical Methods And Lipids In Disease written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-09 with Science categories.
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosis; Measurement of eicosanoids in cancer tissues; Noninvasive Assessment of the Role of Cyclooxygenases in Cardiovascular HealthA Detailed HPLC/MS/MS Method; Lipidomics in Diabetes and the Metabolic Syndrome; LC-MS-MS Analysis of Neutral Eicosanoids; Quantification Of F2-Isoprostanes In Biological Fluids And Tissues As A Measure Of Oxidant Stress; Measurement of Products of Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans; Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography; Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS); Shotgun Lipidomics by Tandem Mass Spectrometry under Data-Dependent Acquisition Control; Identification of Intact Lipid Peroxides by Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS); Quantification of Cardiolipin by Liquid Chromatography Electrospray Ionization Mass Spectrometry.
Regulators And Effectors Of Small Gtpases Part E Gtpases Involved In Vesicular Traffic
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Author :
language : en
Publisher: Elsevier
Release Date : 2001-02-08
Regulators And Effectors Of Small Gtpases Part E Gtpases Involved In Vesicular Traffic written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-02-08 with Medical categories.
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
Rna Interference
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Author : David R. Engelke
language : en
Publisher: Elsevier
Release Date : 2005-02-28
Rna Interference written by David R. Engelke and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-28 with Science categories.
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Stem Cell Tools And Other Experimental Protocols
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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-12
Stem Cell Tools And Other Experimental Protocols written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-12 with Technology & Engineering categories.
This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. - Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering - Presents the latest most innovative technologies - Addresses therapeutic relevance including FDA compliance and tissue engineering
G Protein Pathways
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Author : Ravi Iyengar
language : en
Publisher: Academic Press
Release Date : 2002
G Protein Pathways written by Ravi Iyengar and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cell receptors categories.
This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.
Gene Therapy Methods
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Author : M. Ian Phillips
language : en
Publisher: Academic Press
Release Date : 2002-02-22
Gene Therapy Methods written by M. Ian Phillips and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-02-22 with Medical categories.
This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Parkinson's disease, are topical and are presented in the methods-oriented style popularized by this series. Discusses methods currently used in preclinical and clinical gene therapy Covers the use of adeno-associated virus delivery for treatment of Parkinson's disease
Microrna Methods
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Author : John J. Rossi
language : en
Publisher: Academic Press
Release Date : 2007-08-30
Microrna Methods written by John J. Rossi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-30 with Science categories.
MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process – or stops it – is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.
Lipidomics And Bioactive Lipids Mass Spectrometry Based Lipid Analysis
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-11-26
Lipidomics And Bioactive Lipids Mass Spectrometry Based Lipid Analysis written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-26 with Science categories.
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.
Lipidomics And Bioactive Lipids Lipids And Cell Signaling
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-11-12
Lipidomics And Bioactive Lipids Lipids And Cell Signaling written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-12 with Science categories.
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.
Combinatorial Chemistry
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Author : Guillermo A. Morales
language : en
Publisher: Academic Press
Release Date : 2003
Combinatorial Chemistry written by Guillermo A. Morales and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Amino acid sequence categories.
Combinatorial Chemistry encompasses both the design of compounds for specific pharmacological use and the screening of molecules in high throughput automated tests to find active agents with specific functions. *Analytical techniques *Direct sorting split and pool combinatorial synthesis *Linkers and their applications *Microwave assisted synthesis *Oligosaccharide chemistry *Peptide Synthesis and Screening *Polymer assisted approaches *Small molecule and heterocycle synthesis.