Rice Protocols
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Rice Protocols
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Author : Yinong Yang
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Rice Protocols written by Yinong Yang and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Phenotyping Protocols For Abiotic Stress Tolerance In Rice
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Author : IRRI
language : en
Publisher: IRRI
Release Date :
Phenotyping Protocols For Abiotic Stress Tolerance In Rice written by IRRI and has been published by IRRI this book supported file pdf, txt, epub, kindle and other format this book has been release on with Science categories.
In this manual, we provide detailed protocols for screening rice under the major stresses affecting both rainfed and irrigated rice: submergence, salinity, drought, and the soil problems – excess iron and aluminum. We also include protocols for a new area of abiotic stress research since it is increasingly occurring in farmers’ field due to climate change: combined stresses. Results from these protocols on combined drought + salinity and submergence + salinity indicate that the effects on the rice plant are sometimes different than under individual stresses. The protocols in this manual have been developed and optimized over decades at IRRI. Numerous scientists, technical staff and students have been involved in the development and use of these protocols, and it is often their experiences that have led to improvements, all of which are highly acknowledged. Our objective with this compilation is to provide a resource for researchers around the globe who aim to improve the abiotic stress tolerance of rice as part of the effort to benefit rice farmers in both rainfed and irrigated areas affected by abiotic stresses
Rice Protocols
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Author : Yinong Yang
language : en
Publisher: Humana Press
Release Date : 2012-11-08
Rice Protocols written by Yinong Yang and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-08 with Science categories.
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice
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Author : Fatma Sarsu
language : en
Publisher: Springer
Release Date : 2018-08-09
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice written by Fatma Sarsu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-09 with Science categories.
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
Improved Protocols For Isolated Microspore Culture Of Rice
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Author : Norman L. Darvey
language : en
Publisher:
Release Date : 2000
Improved Protocols For Isolated Microspore Culture Of Rice written by Norman L. Darvey and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Rice categories.
Rice Grain Quality
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Author : Nese Sreenivasulu
language : en
Publisher:
Release Date : 2018
Rice Grain Quality written by Nese Sreenivasulu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Rice categories.
Protocols For Pre Field Screening Of Mutants For Salt Tolerance In Rice Wheat And Barley
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Author : Souleymane Bado
language : en
Publisher: Springer
Release Date : 2016-02-22
Protocols For Pre Field Screening Of Mutants For Salt Tolerance In Rice Wheat And Barley written by Souleymane Bado and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-02-22 with Science categories.
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice
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Author : Fatma Sarsu
language : en
Publisher:
Release Date : 2018
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice written by Fatma Sarsu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Rice categories.
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted - the seedling and flowering stages - with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice
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Author : Ivan Ingelbrecht
language : en
Publisher:
Release Date : 2020-10-08
Pre Field Screening Protocols For Heat Tolerant Mutants In Rice written by Ivan Ingelbrecht and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-08 with Science categories.
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted - the seedling and flowering stages - with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.
Rice Genome Engineering And Gene Editing
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Author : Anindya Bandyopadhyay
language : en
Publisher:
Release Date : 2021
Rice Genome Engineering And Gene Editing written by Anindya Bandyopadhyay and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Botany categories.
This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.