Rna Tagging
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Rna Modification
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-09-13
Rna Modification written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.
The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers.
Rna Tagging
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Author : Manfred Heinlein
language : en
Publisher: Humana
Release Date : 2020-07-25
Rna Tagging written by Manfred Heinlein and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-25 with Science categories.
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
Rna And Dna Editing
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Author : Harold C. Smith
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-25
Rna And Dna Editing written by Harold C. Smith and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-25 with Science categories.
RNA and DNA Editing assembles a team of leading experts who present the latest discoveries in the field alongside the latest models and methodology. In addition, the authors set forth the many open questions and suggest routes for further investigation. Overall, the book serves as a practical guide for professionals in the field who need to understand the interrelationship of RNA and DNA editing with other chemical and biological processes.
Rna Regulation 2 Volumes
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Author : Robert A. Meyers
language : en
Publisher: John Wiley & Sons
Release Date : 2014-05-05
Rna Regulation 2 Volumes written by Robert A. Meyers and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-05 with Science categories.
Based on one of the leading encyclopedic resources in cell and molecular biology worldwide, this two-volume work contains more than 75% new content, not previously published in the Encyclopedia. All the other chapters have been carefully updated. The result is a comprehensive overview of the different functions of the various forms of RNA in living organisms, with each contributor carefully selected and an internationally recognized expert on his or her field. Special focus is on the different forms of expression regulation through RNA, with medical applications in the treatment of diseases -- from cancers and immune responses to infections and aging -- covered in detail. At least 45 of the 55 articles are new content previously not published in the Encyclopedia.
Rna Turnover In Eukaryotes Nucleases Pathways And Analysis Of Mrna Decay
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Author : Lynne E. Maquat
language : en
Publisher: Academic Press
Release Date : 2009-01-30
Rna Turnover In Eukaryotes Nucleases Pathways And Analysis Of Mrna Decay written by Lynne E. Maquat and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-30 with Science categories.
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents
Tag Based Next Generation Sequencing
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Author : Matthias Harbers
language : en
Publisher: John Wiley & Sons
Release Date : 2012-02-13
Tag Based Next Generation Sequencing written by Matthias Harbers and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-13 with Science categories.
Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.
Decoding Neural Circuit Structure And Function
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Author : Arzu Çelik
language : en
Publisher: Springer
Release Date : 2017-07-24
Decoding Neural Circuit Structure And Function written by Arzu Çelik and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-24 with Medical categories.
This book offers representative examples from fly and mouse models to illustrate the ongoing success of the synergistic, state-of-the-art strategy, focusing on the ways it enhances our understanding of sensory processing. The authors focus on sensory systems (vision, olfaction), which are particularly powerful models for probing the development, connectivity, and function of neural circuits, to answer this question: How do individual nerve cells functionally cooperate to guide behavioral responses? Two genetically tractable species, mice and flies, together significantly further our understanding of these processes. Current efforts focus on integrating knowledge gained from three interrelated fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types (“connectomics”) using high-resolution three-dimensional circuit anatomy, and (3) causal testing of how iden tified circuit elements contribute to visual perception and behavior.
Regulatory Rna
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Author : Thomas Dandekar
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Regulatory Rna written by Thomas Dandekar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This book provides an introduction to and an overview of the field of regulatory RNA, focusing on the identification of regulatory elements and motifs in such RNA molecules. Central to the book is the use of appropriate techniques to identify regulatory RNA and regulatory motifs. The prospects for this new and expanding research field - understanding regulatory RNA elements and motifs - are also explored, including new developments, medical applications, and applications in other fields.
Proximity Labeling
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Author : Murat Sunbul
language : en
Publisher: Springer Nature
Release Date : 2019-05-23
Proximity Labeling written by Murat Sunbul and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-23 with Science categories.
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Biophysical Chemical And Functional Probes Of Rna Structure Interactions And Folding Part B
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Author : Daniel Herschlag
language : en
Publisher: Academic Press
Release Date : 2009-11-16
Biophysical Chemical And Functional Probes Of Rna Structure Interactions And Folding Part B written by Daniel Herschlag and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-16 with Science categories.
This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. - Expert researchers introduce the most advanced technologies and techniques in protein structure and folding - Includes techniques on tiling assays