Rnai And Plant Gene Function Analysis
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Rnai And Plant Gene Function Analysis
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Author : Hiroaki Kodama
language : en
Publisher: Humana Press
Release Date : 2011-08-25
Rnai And Plant Gene Function Analysis written by Hiroaki Kodama and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-25 with Science categories.
The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.
Rnai
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Author : Gregory J. Hannon
language : en
Publisher: CSHL Press
Release Date : 2003
Rnai written by Gregory J. Hannon and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Gene silencing categories.
In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.
Non Coding Rnas In Plants
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Author : Volker A. Erdmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-07-20
Non Coding Rnas In Plants written by Volker A. Erdmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-20 with Science categories.
In the most recent years, each of the RNA silencing pathways of plants have appeared to generate ncRNAs with dedicated functions, specialized biological activities and specific functional scopes. RNA silencing plays a crucial role in coordinating the expression, stability, protection and inheritance of eukaryotic genomes. It compromises several mechanisms, that invariably depend on core small non coding RNAs and that achieve dedicated sequence-specific functions. RNA silencing has been recognized to carry critical developmental, stress-response and bodyguard functions be coordinating the expression, protection, stability and inheritance of virtually all eukaryotic genomes. Thus, the ncRNAs encompass a wide set of mechanisms that achieve specialized functions.
Gene Silencing By Rna Interference
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Author : Muhammad Sohail
language : en
Publisher: CRC Press
Release Date : 2004-08-27
Gene Silencing By Rna Interference written by Muhammad Sohail and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08-27 with Medical categories.
Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r
Rnai Technology
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Author : R. K. Gaur
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Rnai Technology written by R. K. Gaur and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Rna Interference
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Author : Dr. Tim Doran
language : en
Publisher: CABI
Release Date : 2009
Rna Interference written by Dr. Tim Doran and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Science categories.
This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Rna Interference Technology
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Author : Krishnarao Appasani
language : en
Publisher: Cambridge University Press
Release Date : 2005-01-17
Rna Interference Technology written by Krishnarao Appasani and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-01-17 with Science categories.
RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
Rna Based Technologies For Functional Genomics In Plants
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Author : Guiliang Tang
language : en
Publisher: Springer Nature
Release Date : 2021-05-13
Rna Based Technologies For Functional Genomics In Plants written by Guiliang Tang and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-13 with Science categories.
This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.
Advances And Challenges Of Rnai Based Technologies For Plants
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Author : Susana Araújo
language : en
Publisher: Frontiers Media SA
Release Date : 2021-07-28
Advances And Challenges Of Rnai Based Technologies For Plants written by Susana Araújo and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-28 with Science categories.
Functional Genomics
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Author : Germana Meroni
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-09-12
Functional Genomics written by Germana Meroni and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-12 with Medical categories.
This book titled "Functional Genomics" contains a selection of chapters focused on crucial topics in functional genomics, from the analysis of the genetic code, to the understanding of the role of the different genes and to the proteomic implications. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Covering all the aspects involved in such a broad theme as functional genomics and in all its applications would be impossible within the same book. The different chapters represent a brief introduction to the topic, connecting the most promising developments in functional genomics technologies, focusing on specific applications in biomedicine, agro-food technologies and zootechniques.