Serial Analysis Of Gene Expression Sage

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Serial Analysis Of Gene Expression Sage

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Author : Kåre Lehmann Nielsen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008

Serial Analysis Of Gene Expression Sage written by Kåre Lehmann Nielsen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.

Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.

Genomics Assisted Crop Improvement

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Author : R.K. Varshney
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-12

Genomics Assisted Crop Improvement written by R.K. Varshney and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-12 with Technology & Engineering categories.

Genomics research has great potential to revolutionize the discipline of plant breeding. This two-volume set provides a critical assessment of genomics tools and approaches for crop breeding. Volume 1, entitled "Genomics Approaches and Platforms", illustrates state-of-the-art genomics approaches and platforms presently available for crop improvement. Volume 2, entitled "Genomics Applications in Crops", compiles crop-specific studies that summarize both the achievements and limitations of genomics research for crop improvement. We hope that these two volumes, while providing new ideas and opportunities to those working in crop breeding, will help graduate students and teachers to develop a better understanding of the applications of crop genomics to plant research and breeding

Analysing Gene Expression

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Author : Stefan Lorkowski
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-06

Analysing Gene Expression written by Stefan Lorkowski and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.

This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.

Genetic Analysis Of Complex Disease

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Author : Jonathan L. Haines
language : en
Publisher: John Wiley & Sons
Release Date : 2007-02-26

Genetic Analysis Of Complex Disease written by Jonathan L. Haines and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-26 with Science categories.

Second Edition features the latest tools for uncovering thegenetic basis of human disease The Second Edition of this landmark publication bringstogether a team of leading experts in the field to thoroughlyupdate the publication. Readers will discover the tremendousadvances made in human genetics in the seven years that haveelapsed since the First Edition. Once again, the editorshave assembled a comprehensive introduction to the strategies,designs, and methods of analysis for the discovery of genes incommon and genetically complex traits. The growing social, legal,and ethical issues surrounding the field are thoroughly examined aswell. Rather than focusing on technical details or particularmethodologies, the editors take a broader approach that emphasizesconcepts and experimental design. Readers familiar with theFirst Edition will find new and cutting-edge materialincorporated into the text: Updated presentations of bioinformatics, multiple comparisons,sample size requirements, parametric linkage analysis, case-controland family-based approaches, and genomic screening New methods for analysis of gene-gene and gene-environmentinteractions A completely rewritten and updated chapter on determininggenetic components of disease New chapters covering molecular genomic approaches such asmicroarray and SAGE analyses using single nucleotide polymorphism(SNP) and cDNA expression data, as well as quantitative trait loci(QTL) mapping The editors, two of the world's leading genetic epidemiologists,have ensured that each chapter adheres to a consistent and highstandard. Each one includes all-new discussion questions andpractical examples. Chapter summaries highlight key points, and alist of references for each chapter opens the door to furtherinvestigation of specific topics. Molecular biologists, human geneticists, geneticepidemiologists, and clinical and pharmaceutical researchers willfind the Second Edition a helpful guide to understanding thegenetic basis of human disease, with its new tools for detectingrisk factors and discovering treatment strategies.

The Dictionary Of Genomics Transcriptomics And Proteomics

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Author : Guenter Kahl
language : en
Publisher: John Wiley & Sons
Release Date : 2015-06-17

The Dictionary Of Genomics Transcriptomics And Proteomics written by Guenter Kahl and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-17 with Science categories.

Now in its fifth edition and for the first time available as an electronic product with all entries cross-linked. This very successful long-seller has once again been thoroughly updated and greatly expanded. It now contains over 13,000 entries, and comprehensively covering genomics, transcriptomics, and proteomics. Each entry contains an extensive explanation, including a comprehensive listing of synonyms and acronyms, and all formulas have been redrawn to create a uniform style, while most of the figures are custom designed for this dictionary. The ultimate reference for all terms in the -omics fields.

Handbook Of Immunohistochemistry And In Situ Hybridization Of Human Carcinomas

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Author : M. A. Hayat
language : en
Publisher: Elsevier
Release Date : 2004-06-16

Handbook Of Immunohistochemistry And In Situ Hybridization Of Human Carcinomas written by M. A. Hayat and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-06-16 with Science categories.

The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book. - The only book available that translates molecular genetics into cancer diagnosis - Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis - Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques - Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis - Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer - Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA - Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References

Research Focus On Gastric Cancer

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Author : Dorothy C. Cardinni
language : en
Publisher: Nova Publishers
Release Date : 2008

Research Focus On Gastric Cancer written by Dorothy C. Cardinni and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.

This book presents important leading-edge research on stomach cancer. Stomach cancer (also called "gastric cancer") is the growth of cancer cells in the lining and wall of the stomach. Stomach cancer represents roughly 2% (25,500) cases of all new cancer cases yearly in the United States, but it is much more common in Japan, Great Britain, South America, and Iceland. It is associated with high salt in the diet, smoking, and low intake of fruits and vegetables. Infection with H. pylori is the main risk factor in about 80% or more of gastric cancers. It is more common in men. A very small percentage of diffuse-type gastric cancers are thought to be genetic. Hereditary Diffuse Gastric Cancer (HDGC) has only recently been identified and research is ongoing, but genetic testing and treatment options are available for families at risk. Metastasis occurs in 80-90% of individuals with stomach cancer, with a five year survival rate of 75% in those diagnosed in early stages and less than 30% of those diagnosed in late stages.

Plant Abiotic Stress Physiology

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Author : Tariq Aftab
language : en
Publisher: CRC Press
Release Date : 2022-02-16

Plant Abiotic Stress Physiology written by Tariq Aftab and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-16 with Science categories.

This two-volume set highlights the various innovative and emerging techniques and molecular applications that are currently being used in plant abiotic stress physiology. Volume 1: Responses and Adaptations focuses on the responses and adaptations of plants to stress factors at the cellular and molecular levels and offers a variety of advanced management strategies and technologies. Volume 2: Molecular Advancements introduces a range of state-of-the-art molecular advances for the mitigation of abiotic stress in plants. With contributions from specialists in the field, Volume 1 first discusses the physiology and defense mechanisms of plants and the various kinds of stress, such as from challenging environments, climate change, and nutritional deficiencies. It goes on to discuss trailblazing management techniques that include genetics approaches for improving abiotic stress tolerance in crop plants along with CRISPR/CAS-mediated genome editing technologies. Volume 2 discusses how plants have developed diverse physiological and molecular adjustments to safeguard themselves under challenging conditions and how emerging new technologies can utilize these plant adaptations to enhance plant resistance. These include using plant-environment interactions to develop crop species that are resilient to climate change, applying genomics and phenomics approaches from the study of abiotic stress tolerance and more. Agriculture today faces countless challenges to meet the rising need for sustainable food supplies and guarantees of high-quality nourishment for a quickly increasing population. To ensure sufficient food production, it is necessary to address the difficult environmental circumstances that are causing cellular oxidative stress in plants due to abiotic factors, which play a defining role in shaping yield of crop plants. These two volumes help to meet these challenges by providing a rich source of information on plant abiotic stress physiology and effective management techniques.

In Vitro Methods In Aquatic Ecotoxicology

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Author : Carmel Mothersill
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-08-14

In Vitro Methods In Aquatic Ecotoxicology written by Carmel Mothersill and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-08-14 with Medical categories.

This book, the first of its kind in this subject area, will focus on in vitro approaches to the study of the toxicology of polluting agents (including heavy metals, radionuclides, micro-organics, estrogenic compounds, and complex mixtures) in the aquatic environment. The importance of in vitro methods is that they allow standardised techniques to be developed and validated for substance and species specific experiments in a controlled way. Also, they allow mechanistic studies without the problems of individual variation between animals and environmental stress.

Textbook Of Personalized Medicine

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Author : Kewal K. Jain
language : en
Publisher: Springer Nature
Release Date : 2020-12-05

Textbook Of Personalized Medicine written by Kewal K. Jain and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-05 with Medical categories.

This book is for personalized medicine as a prescription of specific treatments and therapeutics best suited for an individual and considers genetic as well as environmental factors that influence responses to therapy. Best approaches are described for integration of all available technologies for optimizing the therapy of individual patients. This comprehensive third edition covers the latest advances in personalized medicine and several chapters are devoted to various specialties, particulary cancer which is the largest area of application. The book discusses the development of personalized medicine and various players in it such as companies, academic institutions, the government, and the public as the consumer of healthcare. Additionally, the roles of bioinformatics, electronic health records, and digital technologies for personalized medicine are discussed. Textbook of Personalized Medicine, 3rd Edition serves as a convenient source of information for students at many levels and in a wide range of fields, including physicians, scientists, and decision makers in the biopharmaceutical and healthcare industries.