Single Molecule Microscopy In Neurobiology
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Single Molecule Microscopy In Neurobiology
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Author : Nobuhiko Yamamoto
language : en
Publisher: Humana
Release Date : 2020-04-29
Single Molecule Microscopy In Neurobiology written by Nobuhiko Yamamoto and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-04-29 with Medical categories.
This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.
Cellular Imaging Techniques For Neuroscience And Beyond
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Author : Floris G. Wouterlood
language : en
Publisher: Academic Press
Release Date : 2012-08-08
Cellular Imaging Techniques For Neuroscience And Beyond written by Floris G. Wouterlood and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-08 with Medical categories.
In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).
Imaging In Neuroscience And Development
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Author : Rafael Yuste
language : en
Publisher: CSHL Press
Release Date : 2005
Imaging In Neuroscience And Development written by Rafael Yuste and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Nanoscale Imaging Of Synapses
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Author : U. Valentin Nägerl
language : en
Publisher: Humana
Release Date : 2016-08-23
Nanoscale Imaging Of Synapses written by U. Valentin Nägerl and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
Scanning Electron Microscopy Of Cerebellar Cortex
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Author : Orlando Castejón
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Scanning Electron Microscopy Of Cerebellar Cortex written by Orlando Castejón and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
In a clear exposition this inspirational book shows the in situ three-dimensional morphology of cerebellar neurons, intracortical circuits and synaptic connections that underpin the functioning of cerebellar neurons in networks. It brings together in one volume a new view of the three-dimensional cytoarchitectonic arrangement of the cerebellar cortex. The book shows the cerebellar extrinsic and intrinsic intracortical circuits formed by mossy and climbing fibers as exposed by the cryofracture methods. The high degree of lateral collateralization of these fibers is also displayed providing new insights on the information processing in the cerebellar cortex. Besides, field emission high resolution electron microscopy shows its potential contribution to the study of synaptic morphology. The concluding chapter deals with the contribution of scanning electron microscopy to cerebellar neurobiology. This monograph is an authoritative survey and a must for anyone who is interested in the structure of the central nervous system. It will also appeal to an interdisciplinary audience who wants to learn more about electron microscopy and neurocytology.
Methods In Neurosciences
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Author : P. Michael Conn
language : en
Publisher: Elsevier
Release Date : 2016-07-02
Methods In Neurosciences written by P. Michael Conn and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-02 with Science categories.
Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques. A convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Autoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniques**Statistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining techniques: fluoro-gold, SITS, and silver impregnation**Freezing techniques: freeze fracture and cryoprotection**Combined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques
Cell Biological Applications Of Confocal Microscopy
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Author :
language : en
Publisher: Academic Press
Release Date : 1993-11-17
Cell Biological Applications Of Confocal Microscopy written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-11-17 with Science categories.
Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. Describes proper specimen preparation for obtaining high-quality data and images Covers pitfalls in imaging different specimens and in employing different dyes Contains detailed protocols accessible to new users Chapters are self-contained units, but describe strategies that can be combined to develop new protocols Techniques are illustrated with full-color plates
Imaging Neurons
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Author : Rafael Yuste
language : en
Publisher:
Release Date : 2000
Imaging Neurons written by Rafael Yuste and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
Molecular Neuroscience
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Author : Rusty Lansford
language : en
Publisher:
Release Date : 2014
Molecular Neuroscience written by Rusty Lansford and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Genes categories.
A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.
Fluorescence Microscopy
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Author : Ulrich Kubitscheck
language : en
Publisher: John Wiley & Sons
Release Date : 2017-06-19
Fluorescence Microscopy written by Ulrich Kubitscheck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-19 with Medical categories.
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.