Single Molecule Tools Part A Fluorescence Based Approaches


Single Molecule Tools Part A Fluorescence Based Approaches
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Single Molecule Tools Part A Fluorescence Based Approaches


Single Molecule Tools Part A Fluorescence Based Approaches
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-08-17

Single Molecule Tools Part A Fluorescence Based Approaches written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-17 with Science categories.


Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines



Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods


Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-07-09

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-09 with Science categories.


Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines



Single Molecule Tools


Single Molecule Tools
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Author : Nils G. Walter
language : en
Publisher:
Release Date : 2010

Single Molecule Tools written by Nils G. Walter and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Biosensors categories.




Single Molecule Enzymology Fluorescence Based And High Throughput Methods


Single Molecule Enzymology Fluorescence Based And High Throughput Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2016-10-28

Single Molecule Enzymology Fluorescence Based And High Throughput Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-28 with Medical categories.


Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion



Single Molecule Analysis


Single Molecule Analysis
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Author : Iddo Heller
language : en
Publisher: Humana
Release Date : 2023-11-09

Single Molecule Analysis written by Iddo Heller and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-09 with Science categories.


This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.



Single Molecule Tools


Single Molecule Tools
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Author :
language : en
Publisher:
Release Date : 2010

Single Molecule Tools written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.




Methods In Enzymology


Methods In Enzymology
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Author : Nils G. Walter
language : en
Publisher:
Release Date : 2010

Methods In Enzymology written by Nils G. Walter and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Enzymology categories.




Single Molecule Enzymology Nanomechanical Manipulation And Hybrid Methods


Single Molecule Enzymology Nanomechanical Manipulation And Hybrid Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2017-01-03

Single Molecule Enzymology Nanomechanical Manipulation And Hybrid Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-03 with Science categories.


Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion



Fragment Based Drug Design


Fragment Based Drug Design
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-02-28

Fragment Based Drug Design written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-28 with Medical categories.


There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD



Single Molecule Techniques


Single Molecule Techniques
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Author : Paul R. Selvin
language : en
Publisher:
Release Date : 2008

Single Molecule Techniques written by Paul R. Selvin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Atomic force microscopy categories.


This laboratory manual is the first to take researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. Illustrations.