Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods
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Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-07-09
Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-09 with Science categories.
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Methods In Methane Metabolism Part B
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Author : Amy Rosenzweig
language : en
Publisher: Academic Press
Release Date : 2011-03-17
Methods In Methane Metabolism Part B written by Amy Rosenzweig and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-17 with Science categories.
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. - Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements - The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
Guide To Techniques In Mouse Development Part B
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Author : Paul Wassarman
language : en
Publisher: Academic Press
Release Date : 2010-09-24
Guide To Techniques In Mouse Development Part B written by Paul Wassarman and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-24 with Science categories.
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Synthetic Biology Part B
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Author : Chris Voigt
language : en
Publisher: Academic Press
Release Date : 2011-07-15
Synthetic Biology Part B written by Chris Voigt and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-15 with Science categories.
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation
Protein Engineering For Therapeutics Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-01-25
Protein Engineering For Therapeutics Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-25 with Technology & Engineering categories.
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds - Chapters provide an invaluable resource for academics, researchers and students alike - Iinternational board of authors - This volume is split into sections that cover subjects such as peptides, and scaffolds
Cryo Em Part B 3 D Reconstruction
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30
Cryo Em Part B 3 D Reconstruction written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Research On Nitrification And Related Processes Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-06-15
Research On Nitrification And Related Processes Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Science categories.
The global nitrogen cycle is the one most impacted by mankind. The past decade has changed our view on many aspects of the microbial biogeochemical cycles, including the global nitrogen cycle, which is mainly due to tremendous advances in methods, techniques and approaches. Many novel processes and the molecular inventory and organisms that facilitate them have been discovered only within the last 5 to 10 years, and the process is in progress. Research on Nitrification and Related Processes, Part B provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. - Provides state-of-the-art update on methods and protocols - Deals with the detection, isolation and characterization of macromolecules and their hosting organisms - Deals with the challenges of very diverse environments
The Unfolded Protein Response And Cellular Stress Part B
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Author : P. Michael Conn
language : en
Publisher: Academic Press
Release Date : 2011-01-22
The Unfolded Protein Response And Cellular Stress Part B written by P. Michael Conn and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-01-22 with Science categories.
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights
Constitutive Activity In Receptors And Other Proteins Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-11-24
Constitutive Activity In Receptors And Other Proteins Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-24 with Science categories.
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods
Fragment Based Drug Design
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Author : Lawrence C. Kuo
language : en
Publisher: Academic Press
Release Date : 2011-03-09
Fragment Based Drug Design written by Lawrence C. Kuo and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-09 with Medical categories.
There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD