Stem Cell Protocols
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Stem Cell Protocols
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Author : Ivan N. Rich
language : en
Publisher: Humana Press
Release Date : 2014-11-11
Stem Cell Protocols written by Ivan N. Rich and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-11 with Science categories.
This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.
Human Pluripotent Stem Cells
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Author : Philip H. Schwartz
language : en
Publisher: Humana Press
Release Date : 2011-08-09
Human Pluripotent Stem Cells written by Philip H. Schwartz and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-09 with Science categories.
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.
Leukemia Stem Cells
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Author : César Cobaleda
language : en
Publisher:
Release Date : 2021
Leukemia Stem Cells written by César Cobaleda and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Leukemia categories.
In Vitro Differentiation Of T Cells
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Author : Shin Kaneko
language : en
Publisher: Humana
Release Date : 2020-08-14
In Vitro Differentiation Of T Cells written by Shin Kaneko and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-14 with Science categories.
This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.
Embryonic Stem Cell Protocols
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Author : Kursad Turksen
language : en
Publisher: Humana
Release Date : 2010-11-19
Embryonic Stem Cell Protocols written by Kursad Turksen and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Science categories.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Hematopoietic Stem Cell Protocols
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Author : Kevin D. Bunting
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-01-31
Hematopoietic Stem Cell Protocols written by Kevin D. Bunting and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-31 with Science categories.
This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.
Basic Cell Culture Protocols
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Author : Cheryl D. Helgason
language : en
Publisher: Humana Press
Release Date : 2012-11-20
Basic Cell Culture Protocols written by Cheryl D. Helgason and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-20 with Science categories.
At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
Embryonic Stem Cell Protocols
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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Embryonic Stem Cell Protocols written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Human Embryonic Stem Cell Protocols
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Author : KURSAD TURKSEN
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Human Embryonic Stem Cell Protocols written by KURSAD TURKSEN and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Hematopoietic Stem Cell Protocols
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Author : Christopher A. Klug
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Hematopoietic Stem Cell Protocols written by Christopher A. Klug and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
The ability to highly purify and characterize hematopoietic stem cells (HSC) from mice and humans has opened up an exceedingly rich field of basic science research with enormous clinical potential. Many of the techniques used in st- ies of HSC biology have become more standardized over the last several years, which makes it possible to compile a set of methods that can be used by both seasoned investigators and novices in the stem cell field. We have attempted to be as comprehensive as possible and yet focus on what we perceive to be the most widely used approaches for studies of murine and human HSC. This first edition of Hematopoietic Stem Cell Protocols will therefore have some obvious omissions that were dictated by contemporary circumstances. It is our hope that readers will feel free to contribute their personal suggestions for further chapters as well as on how existing chapters can be improved for future editions. We certainly expect that old approaches will be refined, new assays will be developed, and other animal model and vector systems will be described that will become the new gold standards for future work. Our s- cere thanks goes out to all of the contributors and to those in the stem cell field that have enlarged our thinking and provided new tools to further understand this fascinating cell type.