Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers
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Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers
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Author : Jiby John Mathew
language : en
Publisher: Grin Publishing
Release Date : 2017-07-12
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers written by Jiby John Mathew and has been published by Grin Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-12 with categories.
Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: Citrus, one of the major genes of Rutaceae family and most economically important fruit tree and widely cultivated throughout the country. The Citrus have high nutritional value and medicinal value. The three varieties obtained from various districts in Kerala were used in this study. The phylogenetics is the study of the evolutionary relationships among different species which have common ancestor. These relationships are shown in the form of phylogenetic trees composed of branches which indicate the descendents and nodes which represent the most recent common ancestors. In order to assess the phylogenetic relationships among the 6 samples belongs to the 3 Citrus species (Citrus aurantifolia, Citrus maxima, Citrus limon) a chloroplast gene rbcL and matK was successfully amplified and sequenced. For this study DNA was extracted by using CTAB method. This extracted DNA was analysed by spectrophotometry method for checking purity of DNA.The samples were gel electrophoresed by 1% agarose gel electrophoresis at 80 volts. After electrophoresis the gel is examined in gel documentation system. The DNA band was observed under UV light looking florescent Orangish red colour. The extracted DNA was amplified by PCR method and PCR sample was applied for electrophoresis for checking DNA bands. PCR sample were purified sent for sequencing. Sequences obtained were subjected to editing and alignment using ClustalW programme of Bio Edit and phylogenetic analysis was performed using MEGA software. High similarity observed between selected verities. The locally collected gene sequence-based phylogeny presented here provides support for the early studies of speciation within the Rutaceae. An understanding of the main phylogenetic relationships between Citrus species will help to fine-tune the taxonomy of Rutaceae.
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Ma
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Author : JIBY JOHN. MATHEW
language : en
Publisher:
Release Date : 2017
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Ma written by JIBY JOHN. MATHEW and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers
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Author : Prem Jose Vazhacharickal
language : en
Publisher: GRIN Verlag
Release Date : 2017-07-11
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers written by Prem Jose Vazhacharickal and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-11 with Medical categories.
Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: Citrus, one of the major genes of Rutaceae family and most economically important fruit tree and widely cultivated throughout the country. The Citrus have high nutritional value and medicinal value. The three varieties obtained from various districts in Kerala were used in this study. The phylogenetics is the study of the evolutionary relationships among different species which have common ancestor. These relationships are shown in the form of phylogenetic trees composed of branches which indicate the descendents and nodes which represent the most recent common ancestors. In order to assess the phylogenetic relationships among the 6 samples belongs to the 3 Citrus species (Citrus aurantifolia, Citrus maxima, Citrus limon) a chloroplast gene rbcL and matK was successfully amplified and sequenced. For this study DNA was extracted by using CTAB method. This extracted DNA was analysed by spectrophotometry method for checking purity of DNA.The samples were gel electrophoresed by 1% agarose gel electrophoresis at 80 volts. After electrophoresis the gel is examined in gel documentation system. The DNA band was observed under UV light looking florescent Orangish red colour. The extracted DNA was amplified by PCR method and PCR sample was applied for electrophoresis for checking DNA bands. PCR sample were purified sent for sequencing. Sequences obtained were subjected to editing and alignment using ClustalW programme of Bio Edit and phylogenetic analysis was performed using MEGA software. High similarity observed between selected verities. The locally collected gene sequence-based phylogeny presented here provides support for the early studies of speciation within the Rutaceae. An understanding of the main phylogenetic relationships between Citrus species will help to fine-tune the taxonomy of Rutaceae.
Studies On Genetic Relationships Among Six Varieties Of Jackfruit In Kerala Employing The Matk Gene Using Pcr Technique And Rflp Markers
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Author : Prem Jose Vazhacharickal
language : en
Publisher: GRIN Verlag
Release Date : 2017-01-16
Studies On Genetic Relationships Among Six Varieties Of Jackfruit In Kerala Employing The Matk Gene Using Pcr Technique And Rflp Markers written by Prem Jose Vazhacharickal and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-16 with Science categories.
Scientific Study from the year 2017 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Artocarpus heterophyllus belong to the Moraceae family and are abundant in Western Ghats. The fruit provide two MJ per kg/wet weight of ripe perianth and contain high levels of carbohydrates, protein, starch, calcium and vitamins. Jackfruit has diverse medicinal uses especially for anti-oxidant, anti-inflammatory, antimicrobial, anti-cancer and anti-fungal activity. MatK (maturase K) genes are fast evolving, highly variant regions of plant chloroplast DNA that can serve as potential biomarkers for DNA coding and also in generating primers for plants with identification of unique motif regions. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers, although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using ‘matK gene’ based on PCR technique and RFLP markers. The partial sequence of the ‘matK’ gene of six different Artocarpus varities was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 674 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 674 sites. The nucleotide frequencies are 30.00% (A), 37.69% (T/U), 17.93% (C), and 14.39% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam. has promising leads to further scientific researches and livelihood strategies. The study of matK gene using PCR and RFLP seems to a promising tool in establishing genetic diversity among jackfruit varities. The tree, indigenous to the Western Ghats, is an important source of nutritious food during summer season. Encouragements should be made to the marketing as well as value added food products from this underutilized fruit tree.
Morphological Characterization Of Sweet Potato Ipomoea Batatas Lam Varieties In Kerala An Overview
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Author : Prem Jose Vazhacharickal
language : en
Publisher: Amazon
Release Date :
Morphological Characterization Of Sweet Potato Ipomoea Batatas Lam Varieties In Kerala An Overview written by Prem Jose Vazhacharickal and has been published by Amazon this book supported file pdf, txt, epub, kindle and other format this book has been release on with Reference categories.
Sweet potato (Ipomoea batatas Lam.) is the seventh most important food crop among the root and tuber crops grown in the world which is widely cultivated in tropic, sub tropic and warmer temperate regions. A wide variation exists among sweet potato varieties. The molecular markers Is an important genetic diversity analysis tool for enhancing duplicates identification. The samples were collected based on an elaborative iterative survey as well as traditional knowledge from local people. Thirteen different varieties Ipomoea batatas Lam. varieties (Kuravanpady 1, Kuravanpady 2, Jellipara 1, Jellipara 2, Kottathara, Kadanadu, Kanaka, Aruna, Kanjagad, Cherukizhangu 1, Cherukizhangu 2, Thodupuzha. Palakkad) were used for morphological characterization. Morphological data were collected 60 days after planting based on the descriptors for sweet potato (IBPGR, 1991) in all the 13 samples. The variable scored were twining, plant type, ground cover, vine inter node, vine pigmentation, vine tip pubescence, mature leaf shape, mature leaf size, abaxial leaf vein pigmentation, foliage colour, petiole length, petiole pigmentation, storage root and skin colour. Molecular characterization could be more effectively used in screening varieties among sweet potatoes if coupled with other molecular markers especially ISSR, matK and rbcL markers.
Biodiversity Hotspot Of The Western Ghats And Sri Lanka
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Author : T. Pullaiah
language : en
Publisher: CRC Press
Release Date : 2024-02-20
Biodiversity Hotspot Of The Western Ghats And Sri Lanka written by T. Pullaiah and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-20 with Science categories.
Biodiversity is declining at an alarming rate due to anthropogenic activities around the world. This book is the first volume in the new series Biodiversity Hotspots of the World, which highlights the 36 hotspot regions of the world, regions that were designated as reaping maximum benefit from preservation efforts. This series is our humble attempt to document these hotspots as a conservation and preservation measure. This first volume in the series focuses on the Western Ghats and Sri Lanka, construed as forming a community of species because of their shared biogeographical history. The volume explores the diversity and conservation efforts of the extraordinarily rich species found here, including plants, many of which are found nowhere else in the world; forests, which face tremendous population pressure and have been dramatically impacted by demands for timber and agricultural land; as well as the hotspot’s diverse mammals, birds, insects, and amphibian species, and more. The volumes in this series will be essential resources for researchers and practitioners in the fields of conservation biology, ecology, and evolution.
Evaluation Of Genetic Diversity Of Jackfruit Artocapus Heterophyllus Lam Varieties Based On Sequence Analysis Of The Rbcl Gene
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Author : Prem Jose Vazhacharickal
language : en
Publisher: GRIN Verlag
Release Date : 2017-01-20
Evaluation Of Genetic Diversity Of Jackfruit Artocapus Heterophyllus Lam Varieties Based On Sequence Analysis Of The Rbcl Gene written by Prem Jose Vazhacharickal and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-20 with Science categories.
Scientific Study from the year 2016 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses, especially anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer and anti-fungal activity. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post harvest technology and gaps in supply chain systems. Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients as compared to the common fruits. To find out the morphological variation among jack fruits among Kerala we conducted an elaborative survey and found out the parameters such as tree characteristics, leaf characterises or fruit characteristics of selected samples. Both qualitative and quantitative data are measured using field visits and standard methods and morphological indicators; after analysis of data there is a morphological variation among the jack fruit samples identified. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using the ‘rbcL gene’ based on PCR technique and RFLP markers. The partial sequence of ‘rbcL’ gene of six different Artocarpus varieties was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 651 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 651 sites. The nucleotide frequencies are 27.96% (A), 29.47% (T/U), 19.69% (C), and 22.89% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam has promising leads to further scientific research and livelihood strategies. The study of rbcL gene using PCR and RFLP seems to be a promising tool in establishing genetic diversity among jackfruit varieties.
Breeding And Biotechnology Of Tea And Its Wild Species
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Author : Tapan Kumar Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-02-10
Breeding And Biotechnology Of Tea And Its Wild Species written by Tapan Kumar Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-10 with Science categories.
Tea is an important non-alcoholic beverage plant of the world. Cultivation of tea is very important as it earns revenue for the tea growing nations especially the developing countries such as India. Although conventional breeding is well-established and has contributed significantly for varietal improvement of this plant and other Camellia species with ornamental value, yet applications of biotechnology are required to intervene some of the issues where conventional breeding is restricted particularly for woody plants such as tea. It is note-worthy to mention that some amounts of biotechnology works in several facets of tea and its wild species have also been done. In the present book, a state-of-the-art on various aspects of breeding and biotechnology has been complied in eight chapters. They are: i) Origin and descriptions of health benefits as well as morphological classification as first chapter, ii) Breeding and cytogenetics that comprise with various conventional approaches of varietal improvement of tea along with their genetic resources, iii) Micropropagation which deals with in-depth study of clonal propagation, iv) Somatic embryogenesis along with alternative techniques such as suspension culture, cry-preservation etc. v) Molecular breeding that deals with application of various DNA-based markers, linkage map etc., vi) Genetic transformation and associated factors, vii) Stress physiology complied with various works done in tea along with its wild relatives on abiotic as well as biotic stress, and viii) Functional genomics that describe the various works of molecular cloning and characterizations, differential gene expression, high-throughput sequencing, bioinformatics etc. Importantly, the author has made exclusive tables in most of the chapters that include the summary of the works in particular topic. In a nutshell, the book compiles the work already been done, identifies the problems, analyzes the gaps on breeding and biotechnological works of tea as well as its wild species and discusses the future scope as conclusion. Every effort has been made to include all the published works till June 2013. The book will be a useful resource for post-graduate, doctoral as well post-doctoral students working on tea as well as other woody plants. This will also be useful for the scientists working in the areas of life sciences, genomics, biotechnology and molecular biology.
Tea Genome And Genetics
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Author : Tapan Kumar Mondal
language : en
Publisher: Springer Nature
Release Date : 2020-12-22
Tea Genome And Genetics written by Tapan Kumar Mondal and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-22 with Science categories.
Tea is an important non-alcoholic beverage plant of the world. Cultivation of tea is also commercially very important as it earns huge revenue for the tea growing nations especially the developing countries such as India. Although conventional breeding is well-established and contributes significantly for varietal improvement of this plant and other Camellia species with ornamental value, yet, applications of biotechnology vis a vis genomics are essential to improve the productivity and quality of the tea. This book brings out various aspects of breeding, biotechnology and genomics of tea plants. It covers i) Origin and descriptions of health benefits as well as morphological classification as first chapter, ii) Breeding and cytogenetics that comprise with various conventional approaches of varietal improvement of tea along with their genetic resources, iii) Micro-propagation which deals with in-depth study of clonal propagation, iv) Somatic embryogenesis along with alternative techniques such as suspension culture, cry-preservation etc, v) Molecular breeding that deals with application of various DNA based markers such as discovery of QTL, linkage map etc, vi) Genetic transformation and associated factors, vii) Stress physiology complied various works done in tea along with its wild relatives on abiotic as well as biotic stress and viii) Functional genomics that describes the various works of molecular cloning and characterizations, differential gene expression, high-throughput sequencing, bioinformatics transcriptomics study that described the application of next generation sequencing to discover various genes that are related to various trait of tea, Non-coding RNA which describes the discovery of various non-coding RNA in tea and related genera. The book also discusses recent developments in biotechnology such as metabolomics, proteomics, genome sequence and popular clone varieties of tea crops that are developed across the world. In conclusion, the book collates the work on tea plantations so far, identified the problems, analyzes the gaps on breeding and biotechnological works of tea as well as its wild species and discusses the future scopes as conclusion. The book aims to cover all latest information till june, 2020 . It will be useful resource for post-graduate, doctoral as well post-doctoral students working on tea as well as other woody plants. This will also be useful for the scientists working in the areas of life sciences, genomics, biotechnology and molecular biology.
Breeding Innovations In Underutilized Temperate Fruit Trees
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Author : Giuseppe Ferrara
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-18
Breeding Innovations In Underutilized Temperate Fruit Trees written by Giuseppe Ferrara and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-18 with Science categories.