Studies On Genetic Relationships Among Six Varieties Of Jackfruit In Kerala Employing The Matk Gene Using Pcr Technique And Rflp Markers
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Studies On Genetic Relationships Among Six Varieties Of Jackfruit In Kerala Employing The Matk Gene Using Pcr Technique And Rflp Markers
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Author : Prem Jose Vazhacharickal
language : en
Publisher: GRIN Verlag
Release Date : 2017-01-16
Studies On Genetic Relationships Among Six Varieties Of Jackfruit In Kerala Employing The Matk Gene Using Pcr Technique And Rflp Markers written by Prem Jose Vazhacharickal and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-16 with Science categories.
Scientific Study from the year 2017 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Artocarpus heterophyllus belong to the Moraceae family and are abundant in Western Ghats. The fruit provide two MJ per kg/wet weight of ripe perianth and contain high levels of carbohydrates, protein, starch, calcium and vitamins. Jackfruit has diverse medicinal uses especially for anti-oxidant, anti-inflammatory, antimicrobial, anti-cancer and anti-fungal activity. MatK (maturase K) genes are fast evolving, highly variant regions of plant chloroplast DNA that can serve as potential biomarkers for DNA coding and also in generating primers for plants with identification of unique motif regions. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers, although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using ‘matK gene’ based on PCR technique and RFLP markers. The partial sequence of the ‘matK’ gene of six different Artocarpus varities was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 674 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 674 sites. The nucleotide frequencies are 30.00% (A), 37.69% (T/U), 17.93% (C), and 14.39% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam. has promising leads to further scientific researches and livelihood strategies. The study of matK gene using PCR and RFLP seems to a promising tool in establishing genetic diversity among jackfruit varities. The tree, indigenous to the Western Ghats, is an important source of nutritious food during summer season. Encouragements should be made to the marketing as well as value added food products from this underutilized fruit tree.
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers
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Author : Jiby John Mathew
language : en
Publisher: Grin Publishing
Release Date : 2017-07-12
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Markers written by Jiby John Mathew and has been published by Grin Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-12 with categories.
Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: Citrus, one of the major genes of Rutaceae family and most economically important fruit tree and widely cultivated throughout the country. The Citrus have high nutritional value and medicinal value. The three varieties obtained from various districts in Kerala were used in this study. The phylogenetics is the study of the evolutionary relationships among different species which have common ancestor. These relationships are shown in the form of phylogenetic trees composed of branches which indicate the descendents and nodes which represent the most recent common ancestors. In order to assess the phylogenetic relationships among the 6 samples belongs to the 3 Citrus species (Citrus aurantifolia, Citrus maxima, Citrus limon) a chloroplast gene rbcL and matK was successfully amplified and sequenced. For this study DNA was extracted by using CTAB method. This extracted DNA was analysed by spectrophotometry method for checking purity of DNA.The samples were gel electrophoresed by 1% agarose gel electrophoresis at 80 volts. After electrophoresis the gel is examined in gel documentation system. The DNA band was observed under UV light looking florescent Orangish red colour. The extracted DNA was amplified by PCR method and PCR sample was applied for electrophoresis for checking DNA bands. PCR sample were purified sent for sequencing. Sequences obtained were subjected to editing and alignment using ClustalW programme of Bio Edit and phylogenetic analysis was performed using MEGA software. High similarity observed between selected verities. The locally collected gene sequence-based phylogeny presented here provides support for the early studies of speciation within the Rutaceae. An understanding of the main phylogenetic relationships between Citrus species will help to fine-tune the taxonomy of Rutaceae.
Evaluation Of Genetic Diversity Of Jackfruit Artocapus Heterophyllus Lam Varieties Based On Sequence Analysis Of The Rbcl Gene
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Author : Prem Jose Vazhacharickal
language : en
Publisher: GRIN Verlag
Release Date : 2017-01-20
Evaluation Of Genetic Diversity Of Jackfruit Artocapus Heterophyllus Lam Varieties Based On Sequence Analysis Of The Rbcl Gene written by Prem Jose Vazhacharickal and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-20 with Science categories.
Scientific Study from the year 2016 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses, especially anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer and anti-fungal activity. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post harvest technology and gaps in supply chain systems. Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients as compared to the common fruits. To find out the morphological variation among jack fruits among Kerala we conducted an elaborative survey and found out the parameters such as tree characteristics, leaf characterises or fruit characteristics of selected samples. Both qualitative and quantitative data are measured using field visits and standard methods and morphological indicators; after analysis of data there is a morphological variation among the jack fruit samples identified. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using the ‘rbcL gene’ based on PCR technique and RFLP markers. The partial sequence of ‘rbcL’ gene of six different Artocarpus varieties was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 651 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 651 sites. The nucleotide frequencies are 27.96% (A), 29.47% (T/U), 19.69% (C), and 22.89% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam has promising leads to further scientific research and livelihood strategies. The study of rbcL gene using PCR and RFLP seems to be a promising tool in establishing genetic diversity among jackfruit varieties.
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Ma
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Author : JIBY JOHN. MATHEW
language : en
Publisher:
Release Date : 2017
Studies On Genetic Relationships Among Locally Cultivated Citrus Varieties In Kerala Employing Matk And Rbcl Gene Using Pcr Technique And Rflp Ma written by JIBY JOHN. MATHEW and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Morphological Characterization Of Sweet Potato Ipomoea Batatas Lam Varieties In Kerala An Overview
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Author : Prem Jose Vazhacharickal
language : en
Publisher: Amazon
Release Date :
Morphological Characterization Of Sweet Potato Ipomoea Batatas Lam Varieties In Kerala An Overview written by Prem Jose Vazhacharickal and has been published by Amazon this book supported file pdf, txt, epub, kindle and other format this book has been release on with Reference categories.
Sweet potato (Ipomoea batatas Lam.) is the seventh most important food crop among the root and tuber crops grown in the world which is widely cultivated in tropic, sub tropic and warmer temperate regions. A wide variation exists among sweet potato varieties. The molecular markers Is an important genetic diversity analysis tool for enhancing duplicates identification. The samples were collected based on an elaborative iterative survey as well as traditional knowledge from local people. Thirteen different varieties Ipomoea batatas Lam. varieties (Kuravanpady 1, Kuravanpady 2, Jellipara 1, Jellipara 2, Kottathara, Kadanadu, Kanaka, Aruna, Kanjagad, Cherukizhangu 1, Cherukizhangu 2, Thodupuzha. Palakkad) were used for morphological characterization. Morphological data were collected 60 days after planting based on the descriptors for sweet potato (IBPGR, 1991) in all the 13 samples. The variable scored were twining, plant type, ground cover, vine inter node, vine pigmentation, vine tip pubescence, mature leaf shape, mature leaf size, abaxial leaf vein pigmentation, foliage colour, petiole length, petiole pigmentation, storage root and skin colour. Molecular characterization could be more effectively used in screening varieties among sweet potatoes if coupled with other molecular markers especially ISSR, matK and rbcL markers.
Jackfruit
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Author : Sisir Mitra
language : en
Publisher: CABI
Release Date : 2023-01-06
Jackfruit written by Sisir Mitra and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-06 with Science categories.
Jackfruit (Artocarpus heterophyllus), is a species of tree in the fig, mulberry, and breadfruit family (Moraceae) and is widely esteemed in tropical Asia. The jackfruit tree is well suited to tropical lowlands, and is widely cultivated throughout South and South-East Asia. It is also grown to a limited extent in Australia, USA, East Africa, as well as in Brazil, Mexico and the Caribbean. Producing giant fruits which can reach up to 80kg in size, jackfruit is the largest tree-borne fruit in the world. It is highly versatile, providing food, timber, fuel in addition to medicinal and industrial products. The ripe fruit is sweet and is more often used for desserts. Canned green jackfruit has a mild taste and meat-like texture that lends itself to being called a 'vegetable meat'. Hence, it is growing in popularity due to its use as a vegan meat alternative. Containing information on jackfruit production technology, postharvest management and processing, this is a valuable resource for researchers in horticulture, plant science, and those interested in sustainable food systems.
Breeding And Biotechnology Of Tea And Its Wild Species
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Author : Tapan Kumar Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-02-10
Breeding And Biotechnology Of Tea And Its Wild Species written by Tapan Kumar Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-10 with Science categories.
Tea is an important non-alcoholic beverage plant of the world. Cultivation of tea is very important as it earns revenue for the tea growing nations especially the developing countries such as India. Although conventional breeding is well-established and has contributed significantly for varietal improvement of this plant and other Camellia species with ornamental value, yet applications of biotechnology are required to intervene some of the issues where conventional breeding is restricted particularly for woody plants such as tea. It is note-worthy to mention that some amounts of biotechnology works in several facets of tea and its wild species have also been done. In the present book, a state-of-the-art on various aspects of breeding and biotechnology has been complied in eight chapters. They are: i) Origin and descriptions of health benefits as well as morphological classification as first chapter, ii) Breeding and cytogenetics that comprise with various conventional approaches of varietal improvement of tea along with their genetic resources, iii) Micropropagation which deals with in-depth study of clonal propagation, iv) Somatic embryogenesis along with alternative techniques such as suspension culture, cry-preservation etc. v) Molecular breeding that deals with application of various DNA-based markers, linkage map etc., vi) Genetic transformation and associated factors, vii) Stress physiology complied with various works done in tea along with its wild relatives on abiotic as well as biotic stress, and viii) Functional genomics that describe the various works of molecular cloning and characterizations, differential gene expression, high-throughput sequencing, bioinformatics etc. Importantly, the author has made exclusive tables in most of the chapters that include the summary of the works in particular topic. In a nutshell, the book compiles the work already been done, identifies the problems, analyzes the gaps on breeding and biotechnological works of tea as well as its wild species and discusses the future scope as conclusion. Every effort has been made to include all the published works till June 2013. The book will be a useful resource for post-graduate, doctoral as well post-doctoral students working on tea as well as other woody plants. This will also be useful for the scientists working in the areas of life sciences, genomics, biotechnology and molecular biology.
Coconut Genetic Resources
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Author : Pons Batugal
language : en
Publisher: Bioversity International
Release Date : 2005
Coconut Genetic Resources written by Pons Batugal and has been published by Bioversity International this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Coconut categories.
Protocols For Micropropagation Of Woody Trees And Fruits
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Author : S.Mohan Jain
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-18
Protocols For Micropropagation Of Woody Trees And Fruits written by S.Mohan Jain and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-18 with Technology & Engineering categories.
Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.
Biotechnology Prospects And Applications
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Author : R.K. Salar
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-02-06
Biotechnology Prospects And Applications written by R.K. Salar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-06 with Science categories.
Biotechnology: Prospects and Applications covers the review of recent developments in biotechnology and international authorship presents global issues that help in our understanding of the role of biotechnology in solving important scientific and societal problems for the benefit of mankind and environment. A balanced coverage of basic molecular biology and practical applications, relevant examples, colored illustrations, and contemporary applications of biotechnology provide students and researchers with the tools and basic knowledge of biotechnology. In our effort to introduce students and researchers to cutting edge techniques and applications of biotechnology, we dedicated specific chapters to such emerging areas of biotechnology as Emerging Dynamics of Brassinosteroids Research, Third generation green energy, Bioremediation, Metal Organic Frameworks: New smart materials for biological application, Bioherbicides, Biosensors, Fetal Mesenchymal Stem Cells and Animal forensics. Biotechnology: Prospects and Applications will be highly useful for students, teachers and researchers in all disciplines of life sciences, agricultural sciences, medicine, and biotechnology in universities, research stations and biotechnology companies. The book features broader aspects of the role of biotechnology in human endeavor. It also presents an overview of prospects and applications while emphasizing modern, cutting-edge, and emerging areas of biotechnology. Further, it provides the readers with a comprehensive knowledge of topics in food and agricultural biotechnology, microbial biotechnology, environmental biotechnology and animal biotechnology. The chapters have been written with special reference to the latest developments in above broader areas of biotechnology that impact the biotechnology industry. A list of references at the end of each chapter is provided for the readers to learn more about a particular topic. Typically, these references include basic research, research papers, review articles and articles from the popular literature.