Studies On Initiation Of Protein Synthesis In Yeast
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Studies On Initiation Of Protein Synthesis In Yeast
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Author : Welda Lajean Chaffin
language : en
Publisher:
Release Date : 1969
Studies On Initiation Of Protein Synthesis In Yeast written by Welda Lajean Chaffin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1969 with Proteins categories.
Protein Synthesis And Targeting In Yeast
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Author : Alistair J.P. Brown
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Protein Synthesis And Targeting In Yeast written by Alistair J.P. Brown and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast, S. cerevisiae is proving an ideal model system for studies on protein synthesis and targeting. The topics covered are: - Messenger RNA stability and translation.- The translation apparatus. - Translational control andfidelity. - Protein targeting to the mitochondrion. - Nuclear transport. - The secretory pathway. - Protein folding and degradation. - Protein splicing. Modern and often novel molecular, genetic and biochemical approaches as well as most recent data are provided. The reader will gain a comprehensive view of the current status of the field.
Studies On Yeast In Vitro Protein Synthesis
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Author : Christopher Hal Sissons
language : en
Publisher:
Release Date : 1971
Studies On Yeast In Vitro Protein Synthesis written by Christopher Hal Sissons and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1971 with Eukaryotic cells categories.
Biochemical And Genetic Studies Of Mitochondrial Protein Synthesis In Saccharomyces Cerevisiae
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Author : Changkeun Lee
language : en
Publisher:
Release Date : 2008
Biochemical And Genetic Studies Of Mitochondrial Protein Synthesis In Saccharomyces Cerevisiae written by Changkeun Lee and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Mitochondria categories.
Protein synthesis in archaebacteria and the cytoplasm of eukaryotes is initiated using the initiator methionyl-tRNA (Met-tRNA[subscript i][superscript Met]). In contrast, formylated methionyltRNA (fMet-tRNA[subscript i][superscript Met][subscript f]) is found in eubacteria, and in chloroplasts and mitochondria of eukaryotes, and this formylated initiator tRNA was widely believed to be required for initiation of protein synthesis in those systems. However, the fact that initiation of protein synthesis in yeast mitochondria can occur with unformylated initiator tRNA has changed our perspective about the initiation of mitochondrial protein synthesis. This dissertation is composed of two parts. Part I describes an investigation of the yeast AEP3 gene which was isolated by a genetic screening system in Saccharomyces cerevisiae. The main goal of this part was to discover new accessory factor(s) that might be involved in initiation of protein syntheis of yeast mitochondria when there is no formylation of initiator tRNA and determine how they support the initiation process in Saccharomyces cerevisiae. The synthetic petite genetic screen identified the AEP3 gene. Protein-protein binding assays as well as protein-initiator tRNA binding assays indicate that Aep3p is associated with the initiation process in yeast mitochondrial protein synthesis. This discovery is important because it suggests the possible mechanism by which initiation of protein synthesis in yeast mitochondria occur under conditions where there is no formylation of initiator tRNA. Part II describes a study of the TRM5 gene encoding a tRNA methyltransferase in S. cerevisiae. The TRM5 gene encodes a tRNA (guanine-N1- )-methyltransferase (Trm5p) previously known to methylate guanosine at position 37 (m1G37) in certain cytoplasmic tRNAs in S. cerevisiae. The main goal of this part was to investigate whether Trm5p is also responsible for m1G37 modification of mitochondrial tRNAs. Full-length Trm5p, purified as a fusion protein with maltose-binding protein, exhibited robust methyltransferase activity with tRNA isolated from a [Delta]trm5 mutant strain, as well as with a synthetic mitochondrial tRNA[superscript Met][subscript f] and tRNA[superscript Phe]. High pressure liquid chromatography analysis showed the methylated product to be m1G. Analysis of subcellular fractionation and immunoblotting revealed that the enzyme was localized to both cytoplasm and mitochondria. Our data including the analysis of N-terminal truncation mutants suggest that this tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis.
Studies In Protein Biosynthesis
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Author : Scott Jeffrey Petersen
language : en
Publisher:
Release Date : 1982
Studies In Protein Biosynthesis written by Scott Jeffrey Petersen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Proteins categories.
Transfer Rna In Protein Synthesis
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Author : Dolph L. Hatfield
language : en
Publisher: CRC Press
Release Date : 2018-01-10
Transfer Rna In Protein Synthesis written by Dolph L. Hatfield and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-10 with Medical categories.
Transfer RNA in Protein Synthesis is a comprehensive volume focusing on important aspects of codon usage, selection, and discrimination in the genetic code. The many different functions of tRNA and the specialized roles of the corresponding codewords in protein synthesis from initiation through termination are thoroughly discussed. Variations that occur in the initiation process, in reading the genetic code, and in the selection of codons are discussed in detail. The book also examines the role of modified nucleosides in tRNA interactions, tRNA discrimination in aminoacylation, codon discrimination in translation, and selective use of termination codons. Other topics covered include the adaptation of the tRNA population to codon usage in cells and cellular organelles, the occurence of UGA as a codon for selenocysteine in the universal genetic code, new insights into translational context effects and in codon bias, and the molecular biology of tRNA in retroviruses. The contributions of outstanding molecular biologists engaged in tRNA research and prominent investigators from other scientific disciplines, specifically retroviral research, make Transfer RNA in Protein Synthesis an essential reference work for microbiologists, biochemists, molecular biologists, geneticists, and other researchers involved in protein synthesis research.
Studies On Protein Synthesis By Protoplasts Of Saccharomyces Carlsbergensis
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Author : Siwo Rel de Kloet
language : en
Publisher:
Release Date : 1961
Studies On Protein Synthesis By Protoplasts Of Saccharomyces Carlsbergensis written by Siwo Rel de Kloet and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1961 with Proteins categories.
Molecular Mechanisms Of Protein Biosynthesis
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Author : Herbert Weissbach
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Molecular Mechanisms Of Protein Biosynthesis written by Herbert Weissbach and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Molecular Mechanisms of Protein Biosynthesis is a collection of papers dealing with cell-free systems at the molecular level, including transfer RNA; the initiation, elongation, and termination processes; ribosome structure and function; mRNA translation; and DNA-directed in vitro protein synthesis. A couple of papers review tRNA, aminoacyl-tRNA synthetases, and aspects of ribosome structure. One paper discusses affinity labeling in the study of binding and catalytic sites of large complex and heterogeneous systems such as the ribosome. The investigator should be aware of the chemically reactive or photoactivatible analogue reacting specifically with one or more ribosomal components. This reaction should be determined if it is dependent on the correct binding of the affinity label at the functional site. Another paper describes the series of reactions in protein synthesis as the process by which the ribosome moves relative to the messenger RNA. Other papers discuss messenger RNA and its translation, DNA-dependent cell-free protein synthesis, as well as the genetics of the translational apparatus. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
Studies On The Initiation Of Protein Synthesis In Eukaryotes
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Author :
language : en
Publisher:
Release Date : 1973
Studies On The Initiation Of Protein Synthesis In Eukaryotes written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with categories.
Use Of Protein Synthesis Inhibitors To Study The Biochemistry And Genetics Of The Yeast Ribosome
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Author : Daniel Gordon Schindler
language : en
Publisher:
Release Date : 1976
Use Of Protein Synthesis Inhibitors To Study The Biochemistry And Genetics Of The Yeast Ribosome written by Daniel Gordon Schindler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with Saccharomyces cerevisiae categories.