Protein Synthesis And Targeting In Yeast
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Protein Synthesis And Targeting In Yeast
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Author : Alistair J.P. Brown
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Protein Synthesis And Targeting In Yeast written by Alistair J.P. Brown and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast, S. cerevisiae is proving an ideal model system for studies on protein synthesis and targeting. The topics covered are: - Messenger RNA stability and translation.- The translation apparatus. - Translational control andfidelity. - Protein targeting to the mitochondrion. - Nuclear transport. - The secretory pathway. - Protein folding and degradation. - Protein splicing. Modern and often novel molecular, genetic and biochemical approaches as well as most recent data are provided. The reader will gain a comprehensive view of the current status of the field.
Protein Targeting Transport And Translocation
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Author : Ross Dalbey
language : en
Publisher: Elsevier
Release Date : 2002-04-09
Protein Targeting Transport And Translocation written by Ross Dalbey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-09 with Science categories.
Protein Targeting, Transport, and Translocation presents an in-depth overview on the topic of protein synthesis, covering all areas of protein science, including protein targeting, secretion, folding, assembly, structure, localization, quality control, degradation, and antigen presentation. Chapters also include sections on the history of the field as well as summary panels for quick reference. Numerous color illustrations complement the presentation of material. This book is an essential reference for anyone in biochemistry and protein science, as well as an excellent textbook for advanced students in these and related fields. Basic principles and techniques Targeting adn sorting sequences Protein export in bacteria Membrane protein integration into ER and bacterial membranes Protein translocation across the ER Disulfide bond formation in prokaryotes and eukaryotes Quality control in the export pathway Import of proteins into organelles The secretory pathway Vesicular transport Spectacular color throughout
Studies On Initiation Of Protein Synthesis In Yeast
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Author : Welda Lajean Chaffin
language : en
Publisher:
Release Date : 1969
Studies On Initiation Of Protein Synthesis In Yeast written by Welda Lajean Chaffin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1969 with Proteins categories.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Protein Targeting
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Author : Anthony Pubsley
language : en
Publisher: Academic Press
Release Date : 1989-06-28
Protein Targeting written by Anthony Pubsley and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-06-28 with Science categories.
Protein Targeting reviews different aspects of protein targeting, including similarities and differences in the pathways involved. The early stages of protein export and secretion in prokaryotes and eukaryotes are examined, along with the targeting of mitochondrial and chloroplast proteins as well as some important differences in the biogenesis of the two organelles. Comprised of nine chapters, this book begins with an overview of terminology and basic principles of protein targeting, focusing on protein traffic in eukaryotic and bacterial cells. The reader is then introduced to the basic principles and techniques of protein targeting, including protein identification and posttranslational modification. Subsequent chapters deal with the early stages in the secretory pathway; later stages in the prokaryotic and eukaryotic secretory pathways; targeting of mitochondrial, chloroplast, and peroxisomal proteins; and targeting of nuclear proteins. The book also discusses endocytosis before concluding with a description of applications of protein targeting. This monograph will be of value to scientists and biotechnologists, as well as to students of cell biology, biochemistry, microbiology, and molecular biology.
Recombinant Protein Production In Yeast
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Author : Roslyn M. Bill
language : en
Publisher: Humana Press
Release Date : 2012-03-28
Recombinant Protein Production In Yeast written by Roslyn M. Bill and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-28 with Medical categories.
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Protein Targeting To Mitochondria
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Author : F.U. Hartl
language : en
Publisher: Elsevier
Release Date : 1997-02-24
Protein Targeting To Mitochondria written by F.U. Hartl and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-02-24 with Science categories.
A large number of newly-synthesized polypeptides must cross one or several intracellular membranes to reach their functional locations in the eukaryotic cell. The mechanisms of protein trafficking, in particular the post-translational targeting and membrane translocation of proteins, are of fundamental biological importance and are the focus of intensive research world-wide. For more than 15 years, mitochondria have served as the paradigm organelle system to study these processes. Although key questions, such as how precisely proteins cross a membrane, still remain to be answered, exciting progress has been made in understanding the basic pathways of protein import into mitochondria and the components involved. In addition to a fascinating richness and complexity in detail, the analysis of mitochondrial protein import has revealed mechanistic principles of general significance: Major discoveries include the demonstration of the requirement of an unfolded state for translocation and of the essential role of molecular chaperones on both sides of the membranes in maintaining a translocation-competent conformation and in protein folding after import. It is becoming clear how a polypeptide chain is "reeled" across the membrane in an ATP-dependent process by the functional cooperation of membrane proteins, presumably constituting part of a transmembrane channel, with peripheral components at the trans-side of the membrane. In this volume, eminent experts in the field take the time to review the central aspects of mitochondrial biogenesis. The logical order of the 16 chapters is determined by the sequence of steps during protein import, starting with the events taking place in the cytosol, followed by the recognition of targeting signals, the translocation of precursor proteins across the outer and inner membranes, their proteolytic processing and intramitochondrial sorting, and finally their folding and oligomeric assembly. In addition, the mechanisms involved in the export of mitochondrially encoded proteins as well as recent advances in understanding the division and inheritance of mitochondria will be discussed.
Molecular Biology And Genetic Engineering Of Yeasts
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Author : Henri Heslot
language : en
Publisher: CRC Press
Release Date : 2018-01-18
Molecular Biology And Genetic Engineering Of Yeasts written by Henri Heslot and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Technology & Engineering categories.
Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
Protein Synthesis By A Ribosomal System Derived From Yeast
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Author : Shih Eh Wang
language : en
Publisher:
Release Date : 1967
Protein Synthesis By A Ribosomal System Derived From Yeast written by Shih Eh Wang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1967 with Proteins categories.
Protein Synthesis And Ribosome Structure
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Author : Knud H. Nierhaus
language : en
Publisher: John Wiley & Sons
Release Date : 2004-10-15
Protein Synthesis And Ribosome Structure written by Knud H. Nierhaus and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-10-15 with Science categories.
Knud Nierhaus, who has studied the ribosome for more than 30 years, has assembled here the combined efforts of several scientific disciplines into a uniform picture of the largest enzyme complex found in living cells, finally resolving many decades-old questions in molecular biology. In so doing he considers virtually all aspects of ribosome structure and function -- from the molecular mechanism of different ribosomal ribozyme activities to their selective inhibition by antibiotics, from assembly of the core particle to the regulation of ribosome component synthesis. The result is a premier resource for anyone with an interest in ribosomal protein synthesis, whether in the context of molecular biology, biotechnology, pharmacology or molecular medicine.