Superresolution Optical Microscopy
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Superresolution Optical Microscopy
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Author : Barry R. Masters
language : en
Publisher: Springer Nature
Release Date : 2020-03-21
Superresolution Optical Microscopy written by Barry R. Masters and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-21 with Science categories.
This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
Superresolution Light Microscopy
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Author : Barry R. Masters
language : en
Publisher: Cambridge University Press
Release Date : 2017-05-31
Superresolution Light Microscopy written by Barry R. Masters and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-31 with Technology & Engineering categories.
Super Resolution Optical Imaging And Microscopy
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Author : Junle Qu
language : en
Publisher: John Wiley & Sons
Release Date : 2023-12-15
Super Resolution Optical Imaging And Microscopy written by Junle Qu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-15 with Science categories.
Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.
Label Free Super Resolution Microscopy
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Author : Vasily Astratov
language : en
Publisher: Springer Nature
Release Date : 2019-08-31
Label Free Super Resolution Microscopy written by Vasily Astratov and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-31 with Science categories.
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.
Microscopy Methods In Nanomaterials Characterization
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Author : Sabu Thomas
language : en
Publisher: Elsevier
Release Date : 2017-05-17
Microscopy Methods In Nanomaterials Characterization written by Sabu Thomas and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-17 with Technology & Engineering categories.
Microscopy Methods in Nanomaterials Characterization fills an important gap in the literature with a detailed look at microscopic and X-ray based characterization of nanomaterials. These microscopic techniques are used for the determination of surface morphology and the dispersion characteristics of nanomaterials. This book deals with the detailed discussion of these aspects, and will provide the reader with a fundamental understanding of morphological tools, such as instrumentation, sample preparation and different kinds of analyses, etc. In addition, it covers the latest developments and trends morphological characterization using a variety of microscopes. Materials scientists, materials engineers and scientists in related disciplines, including chemistry and physics, will find this to be a detailed, method-orientated guide to microscopy methods of nanocharacterization. - Takes a method-orientated approach that includes case studies that illustrate how to carry out each characterization technique - Discusses the advantages and disadvantages of each microscopy characterization technique, giving the reader greater understanding of conditions for different techniques - Presents an in-depth discussion of each technique, allowing the reader to gain a detailed understanding of each
Plasmonics And Super Resolution Imaging
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Author : Zhaowei Liu
language : en
Publisher: CRC Press
Release Date : 2017-06-26
Plasmonics And Super Resolution Imaging written by Zhaowei Liu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-26 with Science categories.
Plasmonics is an emerging field mainly developed within the past two decades. Due to its unique capabilities to manipulate light at deep subwavelength scales, plasmonics has been commonly treated as the most important part of nanophotonics. Plasmonic-assisted optical microscopy techniques, especially super-resolution microscopy, have shown tremendous potential and attracted much attention. This book aims to collect cutting-edge studies in various optical imaging technologies with advanced performances that are enabled or enhanced by plasmonics. The basic working principles, development details, and potential future direction and perspectives are discussed. Edited by Zhaowei Liu, a prominent researcher in the field of super-resolution microscopy, this book will be an excellent reference for anyone in the field of nanophotonics, plasmonics, and optical microscopy.
Super Resolution Imaging In Biomedicine
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Author : Alberto Diaspro
language : en
Publisher: CRC Press
Release Date : 2016-11-03
Super Resolution Imaging In Biomedicine written by Alberto Diaspro and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-03 with Science categories.
This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.
Cell Movement In Health And Disease
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Author : Michael Schnoor
language : en
Publisher: Academic Press
Release Date : 2022-03-30
Cell Movement In Health And Disease written by Michael Schnoor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-30 with Science categories.
Cell Movement in Health and Disease brings the several scientific domains related to the phenomena together, establishing a consistent foundation for researchers in this exciting field. The content is presented in four main section. The first explores the foundations of Cell Movement, including overviews of cellular structure, signaling, physiology, motion-related proteins, and the interface with the cellular membrane. The second part covers the biological aspects of cellular movement, starting with chemical and mechanical sensing, describing the types of cell movement, mechanics at cell level, cell physiology, collective behavior, and the connections with the extracellular matrix. The following chapters provide an overview of the molecular machinery involved and cell-type specific movement. The third part of the book is dedicated to the translational aspects of cell movement, highlighting the key conditions associated with cell movement dysfunction, like cell invasion in cancer, wound healing, developmental issues, neurological dysfunctions, and immune response. The final part of the book covers key methods and modeling tools for cell movement research, including predictive mathematical models, in vitro and in vivo methods, biophysical and bioinformatics tools. Cell Movement in Health and Disease is the ideal reference for scientists from different backgrounds converging to expand the understanding of this key cellular process. Cellular and molecular biologists will gain a better understanding of the physical principals operating at cellular level while biophysicist and biomedical engineers will benefit from the solid biology foundation provided by the book. - Combines Biology, Physics and Modeling of cellular movement in one single source - Updated with the current understanding of the field - Includes key research methods for cell movement investigation - Cover translational aspects of cellular movement
Supramolecular Chemistry In Biomedical Imaging
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Author : Stephen Faulkner
language : en
Publisher: Royal Society of Chemistry
Release Date : 2022-04-01
Supramolecular Chemistry In Biomedical Imaging written by Stephen Faulkner and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-01 with Technology & Engineering categories.
There have been many great advances in the field of biomedical imaging in recent years, with supramolecular chemistry playing a key role in the evolution of modern imaging techniques. Non-covalent supramolecular interactions are fundamental to countless biological processes, from host–guest binding to the stabilisation of complex structures. Supramolecular chemistry techniques can be employed to create probes that can be targeted to either exploit or disrupt these interactions, giving the potential for both diagnostic and therapeutic effects. Furthermore, in techniques such as contrast enhanced MRI, controlling the interactions between solvent molecules and the imaging agent is crucial to the development of the technique. With rapid growth in the synthesis and study of molecular imaging agents, the understanding of their associated techniques has sometimes lagged behind. Supramolecular Chemistry in Biomedical Imaging will fill this gap by clarifying the state of current understanding and the nature of the underlying problems inherent to addressing problems in biology. It will cover both the techniques used in imaging and the molecular and supramolecular systems used to exploit them. This publication targets academics coming to the field from mainstream supramolecular chemistry, research graduates and undergraduates interested in supramolecular chemistry, synthesis or imaging agents and imaging techniques for biomedical applications.
Single Particle Nanocatalysis
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Author : Weilin Xu
language : en
Publisher: John Wiley & Sons
Release Date : 2019-02-08
Single Particle Nanocatalysis written by Weilin Xu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-08 with Technology & Engineering categories.
Introduces the detailed basis and recent development of single molecule/particle nanocatalysis based on single molecule techniques This unique book introduces and summarizes the recent development of single molecule/particle nanocatalysis to provide both comprehensive coverage of fundamentals for different methods now in widespread use and the extensive applications in different catalytic systems. Chapters are mainly based on different detection methods, including single molecule fluorescence microscopy, surface plasmon resonance spectroscopy, X-ray microscopy, and surface enhanced Raman spectroscopy. The book also includes numerous basic principles of different methods and application examples and features illustrations that help clarify presentations. Single Particle Nanocatalysis: Fundamentals and Applications starts with the history and development of single molecule techniques for nanocatalysis. It then shows readers how single molecule fluorescence microscopy (SMFM) reveals catalytic kinetics and dynamics of individual nanocatalysts. Next, it examines traditional SMFM-based single molecule nanocatalysis without super-resolution (SR) imaging, before moving on to the topic of SMFM-based SR imaging in single molecule nanocatalysis. Following chapters cover scanning electrochemical microscopy for single particle nanocatalysis; surface plasmon resonance spectroscopy for single particle nanocatalysis/reactions; X-ray-based microscopy of single-particle nanocatalysis; and surface-enhanced Raman spectroscopy for single particle nanocatalysis. The book finishes by introducing some less-practiced techniques for single particle nanocatalysis/electrochemistry. -Presents a systematical and complete introduction to the subject of single particle nanocatalysis?covering all of its fundamentals and applications -Helps readers fully understand the basis, role, and recent development of single molecule nanocatalysis -Teaches researchers how to gain new knowledge to successfully conduct their own studies within this rapidly increasing new area of research Single Particle Nanocatalysis: Fundamentals and Applications is an excellent reference book for experts in this area as well as for general researchers who want to learn how to study nanocatalysis at single molecule/particle level.