Synthetic Biology A Lab Manual
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Synthetic Biology A Lab Manual
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Author : Forster Anthony C
language : en
Publisher: World Scientific
Release Date : 2014-04-16
Synthetic Biology A Lab Manual written by Forster Anthony C and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-16 with Science categories.
Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
Molecular Biology Techniques
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Author : Sue Carson
language : en
Publisher: Academic Press
Release Date : 2019-03-05
Molecular Biology Techniques written by Sue Carson and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-05 with Science categories.
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Human Molecular Biology Laboratory Manual
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Author : Stefan Surzycki
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-15
Human Molecular Biology Laboratory Manual written by Stefan Surzycki and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-15 with Science categories.
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Cell And Molecular Biology Lab Manual
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Author : David A. Thompson
language : en
Publisher:
Release Date : 2009-07-12
Cell And Molecular Biology Lab Manual written by David A. Thompson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-12 with categories.
cell and molecular biology laboratory manual 2009
Plant Molecular Biology A Laboratory Manual
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Author : Melody Clark
language : en
Publisher: Springer
Release Date : 1997
Plant Molecular Biology A Laboratory Manual written by Melody Clark and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Nature categories.
This manual covers the whole range of molecular biology techniques, genetic engineering as well as cytogenetics of plants. Each chapter starts with an introduction into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting. The first part of the book introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning. The second part describes analytical approaches, in particular RAPD and RFLP, while the final part encompasses a variety of gene transfer techniques and both molecular and cytological analysis. The manual will be of great use to both the first-timer and the experienced scientist.
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Manipulation And Expression Of Recombinant Dna
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Author : Sue Carson
language : en
Publisher: Elsevier
Release Date : 2005-12-15
Manipulation And Expression Of Recombinant Dna written by Sue Carson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-15 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Genetics And Molecular Biology Lab Manual Biol 2302
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Author : Gail Begley
language : en
Publisher:
Release Date : 2013
Genetics And Molecular Biology Lab Manual Biol 2302 written by Gail Begley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Recombinant Dna Laboratory Manual
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Author : Judith W. Zyskind
language : en
Publisher: Elsevier
Release Date : 2014-05-12
Recombinant Dna Laboratory Manual written by Judith W. Zyskind and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Science categories.
Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Cell Genetics And Molecular Biology A Lab Manual First Edition
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Author : Michael Perlin
language : en
Publisher: Cognella Active Learning
Release Date : 2017-12-31
Cell Genetics And Molecular Biology A Lab Manual First Edition written by Michael Perlin and has been published by Cognella Active Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-31 with categories.