Synthetic Biology A Lab Manual Second Edition
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Synthetic Biology A Lab Manual Second Edition
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Author : Anthony C Forster
language : en
Publisher: World Scientific
Release Date : 2025-01-10
Synthetic Biology A Lab Manual Second Edition written by Anthony C Forster and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-10 with Science categories.
The first edition of this book was the first manual for laboratory work in the rapidly expanding field of synthetic biology. Based upon a highly successful university course by one of the pioneers in synthetic biology, the manual became particularly popular with students of the enormous annual international Genetically Engineered Machine (iGEM) competition. Questions at the time included the scalability of BioBrick cloning, how to stabilize chromoprotein expression and change the colors, and how to adapt methods for high schools and biohackers. A decade later, this second edition answers these questions with huge BioBrick constructs (front cover), next-generation less-toxic chromoproteins in a kit, and ultraviolet-light-free quantitation by smartphones. Further updates include a computational modeling lab and new avenues in SynBio.
Synthetic Biology A Lab Manual
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Author : Josefine Liljeruhm
language : en
Publisher: World Scientific
Release Date : 2014-04-16
Synthetic Biology A Lab Manual written by Josefine Liljeruhm and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-16 with Science categories.
Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
Antibodies
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Author : Edward Harlow
language : en
Publisher: CSHL Press
Release Date : 1988
Antibodies written by Edward Harlow and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Medical categories.
Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Live Cell Imaging
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Author : Robert D. Goldman
language : en
Publisher: CSHL Press
Release Date : 2005
Live Cell Imaging written by Robert D. Goldman and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Science categories.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Synthetic Biology
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Author : Daniel G. Gibson
language : en
Publisher: Perspectives Cshl
Release Date : 2017
Synthetic Biology written by Daniel G. Gibson and has been published by Perspectives Cshl this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with Science categories.
Synthetic biology involves the rational design and construction of biological components and systemsfrom genetic elements and metabolic pathways to entirely new organisms. Progress in this field has been rapid, and it promises to significantly expand our capabilities in biotechnology, medicine, and agriculture. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology examines the tools and techniques employed by synthetic biologists, how these may be used to develop new drugs, diagnostic approaches, food sources, and clean energy, and what the field of synthetic biology has taught us about natural living systems. The contributors discuss advances in DNA synthesis and assembly, genome editing (e.g., CRISPR/Cas9), and artificial genetic systems. Progress in designing complex genetic switches and circuits, expanding the genetic code, modifying cellular organization, producing proteins using cell-free systems, and developing biodesign automation tools is also covered. The authors also explore ways to produce new organisms and products that have particular attributesfor example, microbial "molecular factories," synthetic organs and tissues, and plants with novel traits. This volume is an essential resource for molecular, cell, and systems biologists who seek to engineer living systems for human benefit.
Catalog Of Copyright Entries Third Series
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Author : Library of Congress. Copyright Office
language : en
Publisher: Copyright Office, Library of Congress
Release Date : 1978
Catalog Of Copyright Entries Third Series written by Library of Congress. Copyright Office and has been published by Copyright Office, Library of Congress this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Copyright categories.
Law Enforcement In The United States
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Author : James A. Conser
language : en
Publisher: Jones & Bartlett Learning
Release Date : 2005
Law Enforcement In The United States written by James A. Conser and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Law categories.
Law Enforcement in the United States, Second Edition presents a unique balance of theory, history, and practice of American law enforcement. It provides readers with updated, important information ranging from the evolution and theory of social control to the training, function, and strategies involved in modern policing. The authors also examine the gray areas of law enforcement, ethics, forces in society that impact policing, and the laws governing police behavior.
Medical Biochemistry
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Author : N. V. Bhagavan
language : en
Publisher: Jones & Bartlett Learning
Release Date : 1992
Medical Biochemistry written by N. V. Bhagavan and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
Thoroughly updated and in a new two-color format, this well- respected text presents the fundamentals of biochemistry and related topics to students pursuing a one- or two-semester course in pre-med biochemistry or medical programs. The second edition is equally applicable to other health-related fields such as clinical chemistry, medical technology or pharmacology. Medical Biochemistry, Fourth Edition, focuses on the foundations and clinically relevant applications of normal human biochemistry and pathology. Abundantly illustrated with four-color plates.Revised chapters on molecular biology reflect the latest research in the fieldTwo color throughout with four color platesReference quality appendices include practical information on clinical lab parameters used to diagnose a range of diseases