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Techniques In Cell Cycle Analysis


Techniques In Cell Cycle Analysis
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Techniques In Cell Cycle Analysis


Techniques In Cell Cycle Analysis
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Author : Joe W. Gray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-24

Techniques In Cell Cycle Analysis written by Joe W. Gray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-24 with Science categories.


Quantification of the proliferative characteristics of normal and malignant cells has been of interest to oncolo gists and cancer biologists for almost three decades. This interest stems from (a) the fact that cancer is a disease of uncontrolled proliferation, (b) the finding that many of the commonly used anticancer agents are preferentially toxic to cells that are actively proliferating, and (c) the observa tion that significant differences in proliferation characteristics exist between normal and malignant cells. Initially, cell cycle analysis was pursued enthusiastically in the hope of gener ating information useful for the development of rational cancer therapy strategies; for example, by allowing identi fication of rapidly proliferating tumors against which cell cycle-specific agents could be used with maximum effec tiveness and by allowing rational scheduling of cell cyc- specific therapeutic agents to maximize the therapeutic ratio. Unfortunately, several difficulties have prevented realiza tion of the early promise of cell cycle analysis: Proliferative patterns of the normal and malignant tissues have been found to be substantially more complex than originally an ticipated, and synchronization of human tumors has proved remarkably difficult. Human tumors of the same type have proved highly variable, and the cytokinetic tools available for cell cycle analysis have been labor intensive, as well as somewhat subjective and in many cases inapplicable to humans. However, the potential for substantially improved cancer therapy remains if more accurate cytokinetic infor mation about human malignancies and normal tissues can be obtained in a timely fashion.



Techniques In Cell Cycle Analysis


Techniques In Cell Cycle Analysis
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Author : Joe W. Gray
language : en
Publisher:
Release Date : 2014-09-01

Techniques In Cell Cycle Analysis written by Joe W. Gray and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-01 with categories.




The Cell Cycle


The Cell Cycle
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Author : David Owen Morgan
language : en
Publisher: New Science Press
Release Date : 2007

The Cell Cycle written by David Owen Morgan and has been published by New Science Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.


Cell division is a central biological process: it yields the cells required for development and growth, and supplies the replacement cells to repair and maintain old or damaged tissue. This book gives the students a complete overview of the process of cell division - from chromosome division, through mitosis, cytokinesis, and meiosis.



Laboratory Techniques In Cellular And Molecular Medicine


Laboratory Techniques In Cellular And Molecular Medicine
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Author : Hajdúch, Marián
language : en
Publisher: Palacky University Olomouc
Release Date :

Laboratory Techniques In Cellular And Molecular Medicine written by Hajdúch, Marián and has been published by Palacky University Olomouc this book supported file pdf, txt, epub, kindle and other format this book has been release on with Medical categories.


The methodological book ""Laboratory techniques in cellular and molecular medicine"" is intended for students of bachelor, master, and doctoral study programmes at faculties of science, medicine, and veterinary medicine, as well as for laboratory technicians interested in methodological approaches of contemporary cellular and molecular medicine. The book does not aim to provide a comprehensive overview of the current state of the art in cellular and molecular medicine, that would be a superhuman task. The aim of the 56-member author team is to provide readers with an overview of the methods established and tested at the Institute of Molecular and Translational Medicine of the Faculty of Medicine of Palacký University Olomouc – to describe the methodological principles and their practical application. It focuses both on basic methods, whose principles are used by the most modern methods, and on special methods, reflecting the laboratory experience and specialisation of individual authors. The 52 chapters describe the work with cells and microorganisms, bioengineering manipulations of nucleic acids, the search for biomarkers, detection at the level of DNA, RNA, protein, organelle, and whole cell, and the use of fluorescent and radioactive labeling. To aid comprehension, the description of the methods is illustrated by figures and diagrams. Each chapter is followed by troubleshooting tips. The book promotes the best laboratory practice to increase the reproducibility of results in biomedicine.



Techniques


Techniques
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-18

Techniques written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-18 with Science categories.


In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy.



Handbook Of Plant Ecophysiology Techniques


Handbook Of Plant Ecophysiology Techniques
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Author : M. J. Reigosa Roger
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-05-08

Handbook Of Plant Ecophysiology Techniques written by M. J. Reigosa Roger and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-08 with Science categories.


The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 François Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research.



Cell Culture


Cell Culture
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Author :
language : en
Publisher: Academic Press
Release Date : 1988-06-28

Cell Culture written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-06-28 with Science categories.


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.



Advances In Plant Ecophysiology Techniques


Advances In Plant Ecophysiology Techniques
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Author : Adela M. Sánchez-Moreiras
language : en
Publisher: Springer
Release Date : 2018-08-17

Advances In Plant Ecophysiology Techniques written by Adela M. Sánchez-Moreiras and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-17 with Science categories.


This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.



Flow Cytometry Applications In Cell Culture


Flow Cytometry Applications In Cell Culture
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Author : Mohamed Al-Rubeai
language : en
Publisher: CRC Press
Release Date : 2020-07-24

Flow Cytometry Applications In Cell Culture written by Mohamed Al-Rubeai and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.


This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.



Analytical Use Of Fluorescent Probes In Oncology


Analytical Use Of Fluorescent Probes In Oncology
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Author : Elli Kohen
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Analytical Use Of Fluorescent Probes In Oncology written by Elli Kohen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.


Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in biomedicine through the convergence of these approaches. When applied to in vivo and in situ studies, fluorescence and related optical methods enable us to explore within tissues, cells and organelles photon effects previously understood only in solution photochemistry. Processes which can be studied at the molecular level by photophysics, photochemistry and physical chemistry can be evaluated in living tissue by fluorescence spectroscopy and imaging at the intracellular level in terms of structure and function. Thus, fluorescence adds a new dimension to cell biology and physiology. This approach is now supported by a full and versatile, rapidly growing armamentarium of new selective probes for organelles, enzymes, cations, cytoskeleton and metabolic control.