The Biology Of Baculoviruses
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The Biology Of Baculoviruses
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Author :
language : en
Publisher:
Release Date : 1986
The Biology Of Baculoviruses written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Baculoviruses categories.
Biology Of Baculoviruses
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Author : Robert R. Granados
language : en
Publisher: Springer
Release Date : 1986
Biology Of Baculoviruses written by Robert R. Granados and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Science categories.
V.1 - Biological properties and molecular biology: Historical perspectives; Taxonomy and identification of baculoviruses; Ultrastructure of baculoviruses; In vivo infection and replication of baculoviruses; In vitro replication of baculoviruses; Baculovirus structural proteins and protein synthesis; Current developments in baculovirus serology; Persistent baculovirus infections; Specificity and safety of baculoviruses; Evolution of occluded baculoviruses; The genetics of baculoviruses; Organization and expression of the baculovirus genome; v.2 - Practical application for insect control: In vivo and in vitro bioassay methods for baculoviruses; In vivo production of baculoviruses; Cell culture methods for large-scale propagation of baculoviruses; Ecology and ens, chemical pesticides, and parasitoids; Formulation and application of baculoviruses; Use of baculoviruses in pest management programs; Registration of baculoviruses as pesticides; Practical factors influencing the utilization of baculoviruses as pesticides; Insect resistance to baculoviruses.
The Baculoviruses
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Author : Lois K. Miller
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
The Baculoviruses written by Lois K. Miller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.
The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.
The Molecular Biology Of Baculoviruses
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Author : Walter Doerfler
language : en
Publisher: Springer
Release Date : 1986-12
The Molecular Biology Of Baculoviruses written by Walter Doerfler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986-12 with Health & Fitness categories.
The pathology caused by baculoviruses in insect popula tions was described centuries ago, notably in the larvae of insects such as the silkworm (Bombyx mori) which has been appreciated for the quality and beauty of its products. In the 1940s baculoviruses and their structure and physiolo gy were intensively investigated, particularly by Bergold's group in Tiibingen. The following decades saw excellent progress, laying a solid virological base for later investiga tions on the system. Further studies mushroomed in the 1970s with the advent of tissue culture systems for insect cells which eventually facilitated the molecular biological approach that came to the fore in the 1980 s. One of the reasons for pursuing research on the baculo virus system was the prospect of eventually using these vi ruses as insect pest control agents. While this practical as pect may appeal to many, molecular biologists had addi tional reasons to be interested in baculoviruses. Here was a large DNA viral genome, probably fraught with problems of replication and regulation that hopefully would open inroads into the molecular biology of interesting insect cell systems. In the days when genetechnology promises laurels, and after several virus systems had been skilfully exploited as highly efficient eukaryotic expression vectors, it came as no surprise that baculoviruses were also investigated in that respect. Indeed, the Autographa californica nuclear po lyhedrosis virus became a good vector. Insect cells also seem to collaborate in modifying and processing the gene technologically synthesized polypeptides.
The Baculoviruses
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Author : Lois K Miller
language : en
Publisher: Springer
Release Date : 2014-01-15
The Baculoviruses written by Lois K Miller and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Baculovirus And Insect Cell Expression Protocols
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Author : David W. Murhammer
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-04-15
Baculovirus And Insect Cell Expression Protocols written by David W. Murhammer and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-15 with Medical categories.
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
The Baculovirus Expression System
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Author : Linda King
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Baculovirus Expression System written by Linda King and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.
Baculovirus Expression Protocols
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Author : Christopher D. Richardson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Baculovirus Expression Protocols written by Christopher D. Richardson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
Baculovirus
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Author : Manon M.J. Cox
language : en
Publisher: Humana
Release Date : 2024-08-24
Baculovirus written by Manon M.J. Cox and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-08-24 with Science categories.
This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Baculovirus: Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.
Biology Of Baculoviruses
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Author : Robert R. Granados
language : en
Publisher: Springer
Release Date : 1986
Biology Of Baculoviruses written by Robert R. Granados and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Science categories.