The Genetic Regulation Of Pathogenicity In Candida Parapsilosis
DOWNLOAD
Download The Genetic Regulation Of Pathogenicity In Candida Parapsilosis PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get The Genetic Regulation Of Pathogenicity In Candida Parapsilosis book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
The Genetic Regulation Of Pathogenicity In Candida Parapsilosis
DOWNLOAD
Author : Siobhán A. Turner
language : en
Publisher:
Release Date : 2018
The Genetic Regulation Of Pathogenicity In Candida Parapsilosis written by Siobhán A. Turner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Biofilms categories.
Fungal Pathogenesis In Humans
DOWNLOAD
Author : Fernando Leal
language : en
Publisher: MDPI
Release Date : 2019-05-27
Fungal Pathogenesis In Humans written by Fernando Leal and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-27 with Science categories.
Dear Colleagues, Cancer survival rates and successful organ transplantation in patients continues to increase due to improvements in early diagnosis and treatments. Since immuno-suppressive therapies are frequently used, the mortality rate due to secondary infections has become an ever-increasing problem. Opportunistic fungal infections are probably the deadliest threat to these patients due to their difficult early diagnosis, the limited effect of antifungal drugs and the appearance of resistances. In recent years, a considerable effort has been devoted to investigating the role of many virulence traits in the pathogenic outcome of fungal infections. New virulence factors (hypoxia adaptation, CO2 sensing, pH regulation, micronutrient acquisition, secondary metabolites, immunity regulators, etc.) have been reported and their molecular mechanisms of action are being thoroughly investigated. The recent application of gene-editing technologies such as CRISPr-Cas9, has opened a whole new window to the discovery of new fungal virulence factors. Accurate fungal genotyping, Next Generation Sequencing and RNAseq approaches will undoubtedly provide new clues to interpret the plethora of molecular interactions controlling these complex systems. Unraveling their intimate regulatory details will provide insights for a more target-focused search or a rational design of more specific antifungal agents. This Special Issue is show significant discoveries, proofs of concept of new theories or relevant observations in fungal pathogenesis and its regulation. Dr. Fernando Leal Guest Editor
Generation And Characterization Of Candida Parapsilosis Over Expression Mutant Strains
DOWNLOAD
Author : S.E. Pu00e1l
language : en
Publisher:
Release Date : 2017
Generation And Characterization Of Candida Parapsilosis Over Expression Mutant Strains written by S.E. Pu00e1l and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Candida parapsilosis is an opportunistic human fungal pathogen, threatening a wide range of patient group ranging from low birth-weight neonates to the critically ill and severely immune suppressed patients treated at intensive care units. Furthermore, C. parapsilosis infection rates can even exceed the candidaemia episodes caused by C. albicans at certain geographical regions. Therefore, excessive research is now focused on the investigation of C. parapsilosisu2019 pathogenesis. In order to search for genes potentially influencing the virulence of C. parapsilosis, we aimed to generate and characterize over-expression mutant strains of ORFs that, according to our preliminary experiments, are thought to influence fungal pathogenesis.To identify such set of genes, transcriptional analysis of fungi co-incubated with THP-1 human monocytes was performed. Differentially expressed kinases and transcriptional factors and additional orthologous genes of C. albicans virulence regulatory ORFs (adherence, biofilm formation, antifungal resistance) were sentenced to over-expression. We used the Thermo Fisher Scientific Gateway cloning technology to create over-expression mutant strains. Transformants were validated by colony-PCR, Southern blot and real-time PCR. The fitness of the verified mutants was monitored in vitro at various temperatures and under conditions that are thought to influence the virulence of C. parapsilosis. These included different pH conditions, minimal and complex media and also solid media supplemented with osmotic, oxidative or cell wall stressors. Fitness under metal iron limiting conditions was also examined.During our work, we successfully generated 36 over-expression mutant strains with 2 parallels and added unique 20-mer nucleotide barcode sequences to each in order to be able to identify them during later co-infection studies. Integration of the over-expression constructs was confirmed by both colony PCR and real-time PCR. To exclude the possibility of ectopic integrations, Southern-blot analysis was also applied. We confirmed that all mutant strains contained only one copy of the integrating constitutive promoter containing over-expression cassette. Thereafter, all available mutant strainsu2019 viability was examined under the aforementioned conditions. During the characterization, altered phenotypes were detected mainly in the presence of SDS, Calcofluor white and Congo Red. Under these conditions, mutants showed growth disabilities when compared to the parental strain. In this study, we successfully generated 36 C. parapsilosis over-expression mutant strains. According to our results, several of these showed a phenotype different from the wild type under conditions that are thought to impact the pathogenicity of this species. In order to supplement this hypothesis we further aim to perform virulence studies including both in vitro and in vivo infection models.This project was funded by NKFIH K123952 and GINOP-2.3.2-15-2016-00015.
Candida
DOWNLOAD
Author : Christophe d'. Enfert
language : en
Publisher: Caister Academic Press Limited
Release Date : 2007
Candida written by Christophe d'. Enfert and has been published by Caister Academic Press Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.
Candida species are important human pathogens that are best known for causing opportunist infections in immunocompromised hosts (e.g. transplant patients, AIDS sufferers, cancer patients). Infections are difficult to treat and can be very serious. 30-40% of systemic infections result in death. The sequencing of the genome of C. albicans and those of several other medically-relevant Candida species has provided a major impetus for Candida comparative and functional genomic analyses. These have provided a fascinating insight into the molecular and cellular biology of these fungi, and these should pave the way for the development of more sensitive diagnostic strategies and novel antifungal therapies. In this timely book, international experts provide comprehensive and authoritative reviews of these important organisms. The topics covered include: genome dynamics of C. albicans; molecular, epidemiological, and population studies of C. albicans; the parasexual cycle in C. albicans; comparative genomics of the hemiascomycetes group and of different Candida species; databases and the challenges that their curators are facing; transcriptome analysis; proteomics; molecular evolution of the genetic code; stress responses; regulation of morphogenesis; cell wall biology; interaction with host cells; analysis of gene function; antifungal resistance mechanisms; and much more. This book is essential reading for all Candida genome and molecular biology researchers and a recommended text for scientists working on fungal genomics and molecular biology.
Candida And Candidiasis
DOWNLOAD
Author : Richard A. Calderone
language : en
Publisher: American Society for Microbiology Press
Release Date : 2011-12-07
Candida And Candidiasis written by Richard A. Calderone and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-07 with Medical categories.
The underlying mechanisms of Candida and candidiasis and promising new directions in drug discovery and treatment. • Reviews all aspects of this common fungal pathogen and its impact on human health, from the basic biology of Candida albicans to the clinical management of candidiasis. • Reviews the latest basic and clinical research, focusing on findings in genome variability, host-pathogen interactions, antifungal resistance and drug discovery, and diagnostics to foster better understanding and treatment of candidiasis. • Examines recent discoveries that have shed light on morphogenesis and the cell cycle, including how new findings on host responses may have applications for the diagnosis of blood-borne candidiasis.
Fungal Metabolites
DOWNLOAD
Author : Jean-Michel Mérillon
language : en
Publisher:
Release Date :
Fungal Metabolites written by Jean-Michel Mérillon and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with Fungi categories.
Pathogenic Yeasts
DOWNLOAD
Author : Ruth Ashbee
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-10-27
Pathogenic Yeasts written by Ruth Ashbee and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-27 with Science categories.
Mycological studies of yeasts are entering a new phase, with the sequencing of multiple fungal genomes informing our understanding of their ability to cause disease and interact with the host. At the same time, the ongoing use of traditional methods in many clinical mycology laboratories continues to provide information for the diagnosis and treatment of patients. This volume reviews various aspects of pathogenic yeasts and what is known about their molecular and cellular biology and virulence, in addition to looking at clinical and laboratory findings. As each chapter is written by a leading expert in the field, this book summarizes in one volume much of the latest research on several pathogenic yeasts, including Candida, Cryptococcus, Malassezia and yeasts of emerging importance. The importance of laboratory diagnosis, antifungal susceptibility testing, antifungal resistance and yeast diseases in animals are reviewed.
Diagnostics To Pathogenomics Of Sexually Transmitted Infections
DOWNLOAD
Author : Sunit Kumar Singh
language : en
Publisher: John Wiley & Sons
Release Date : 2018-12-06
Diagnostics To Pathogenomics Of Sexually Transmitted Infections written by Sunit Kumar Singh and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-06 with Science categories.
Comprehensively explores sexually transmitted diseases, from epidemiology, causative pathogens, clinical impact, and immunology, to management strategies utilizing new strategies of genomics and next-generation diagnostic tools Sexually transmitted infections (STI) are very common worldwide. More than 20 different STIs have been identified, and about 19 million men and women are infected each year in the United States alone. This book looks at the complete picture of common STIs— how they form, evolve, and transmit, as well as how they can be treated and managed with modern techniques, medicines, and tools. Diagnostics to Pathogenomics of Sexually Transmitted Infections runs the spectrum of discussion ranging from introduction of causative pathogen, their pathogenesis to epidemiology, immunology, to anatomy and physiology of human genitalia and management strategies. The book offers in-depth chapter coverage on effect of probiotics on reproductive health; mucosal immunity in sexually transmitted infections; the role of circumcision in preventing STIs; Human Immunodeficiency Virus (HIV); genital herpes; molluscum contagiosum; genital warts; chlaymydia trachomatis; donovanosis; gonorrhoea; treponematoses; genital mycoplasms; bacterial vaginosis; vulvovaginal candidiasis; chlaymydia; scabies; chancroid, yeast infections; and more. Comprehensively compiles most of the major sexually transmitted infections Presents updated information on clinical aspects of sexually transmitted infections Examines the priorities in pathogenesis of human sexually transmitted infections and discusses new strategies of genomics and next-generation diagnostic tools used for detection of such pathogens Explores the future of rapid molecular diagnostic techniques and the challenges posed in the diagnosis of human STIs Includes bench to bedside content that will appeal to both basic and clinical researchers By offering the latest knowledge about recent advances in sexually transmitted infections in an interdisciplinary fashion, Diagnostics to Pathogenomics of Sexually Transmitted Infections is the perfect book for virologists, microbiologists, infectious disease experts, vaccinologists, biomedical researchers, clinicians, pharmacologists, and public health specialists.
Fungal Physiology And Immunopathogenesis
DOWNLOAD
Author : Marcio L. Rodrigues
language : en
Publisher: Springer Nature
Release Date : 2019-09-03
Fungal Physiology And Immunopathogenesis written by Marcio L. Rodrigues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-03 with Medical categories.
This volume offers an overview of the various aspects involved in the ability of fungi to damage host cells, and discusses cutting-edge approaches to the study of fungal pathogenesis. The first chapter illustrate the key roles of glycans and pigments, the most abundant surface components in fungal cells, in their interactions with host cells. The connections between cellular physiology and fungal pathogenesis are then discussed in the following chapters. Physiology-related processes affecting pathogenesis include fungal secretion, morphological transitions, and response to light. In turn, the book illustrates mechanisms of damage to host cells using the Histoplasma capsulatum model of infection, and reviews the use of transcriptomic approaches to understand the mechanisms of interaction between fungal cells and host tissues. After a discussion of the immunological mechanisms underlying host susceptibility to fungal infections, the book’s closing contribution reviews the mechanisms of interaction between fungi and other microbes, and the impact of this association on fungal pathogenesis. Given its scope, the book will appeal to scientists in the fields of mycology, microbiology, infectious diseases, biology and medicine.
Advances In Candida Albicans
DOWNLOAD
Author :
language : en
Publisher: BoD – Books on Demand
Release Date : 2021-11-17
Advances In Candida Albicans written by and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-17 with Medical categories.
Candida albicans, a fungal pathobiont, is the major component of the microbiota communities in healthy adults. It resides in the host’s gastrointestinal tract and mouth and can become pathogenic via overgrowth under a variety of conditions. This book reviews recent knowledge and the latest research on C. albicans, including the mechanism of candidiasis infection, host response, antifungal strategies, biofilms, genetics, and molecular epidemiology of immune responses.