The Optical Clearing Method
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The Optical Clearing Method
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Author : Luís Manuel Couto Oliveira
language : en
Publisher: Springer Nature
Release Date : 2019-11-27
The Optical Clearing Method written by Luís Manuel Couto Oliveira and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-27 with Science categories.
This book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed.
Handbook Of Tissue Optical Clearing
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Author : Valery V. Tuchin
language : en
Publisher: CRC Press
Release Date : 2022-02-04
Handbook Of Tissue Optical Clearing written by Valery V. Tuchin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-04 with Science categories.
Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book
Optical Clearing Of Tissues And Blood
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Author : Valeriĭ Viktorovich Tuchin
language : en
Publisher: SPIE-International Society for Optical Engineering
Release Date : 2006
Optical Clearing Of Tissues And Blood written by Valeriĭ Viktorovich Tuchin and has been published by SPIE-International Society for Optical Engineering this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Medical categories.
This book describes an optical clearing method based on reversible reduction of tissue scattering due to refractive index matching of scatterers and ground matter. This technique, which has been of great interest for research and application in the last decade, is a promising technique for future developments in the fields of tissue imaging, spectroscopy, phototherapy, and laser surgery.
Second Harmonic Generation Imaging
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Author : Francesco S. Pavone
language : en
Publisher: Taylor & Francis
Release Date : 2016-04-19
Second Harmonic Generation Imaging written by Francesco S. Pavone and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
Advanced Biophotonics
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Author : Ruikang K. Wang
language : en
Publisher: Taylor & Francis
Release Date : 2016-04-19
Advanced Biophotonics written by Ruikang K. Wang and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.
Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and
Tissue Optics
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Author : Valery Tuchin
language : en
Publisher:
Release Date : 2015
Tissue Optics written by Valery Tuchin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Diagnostic imaging categories.
This third edition of the biomedical optics classic Tissue Optics covers the continued intensive growth in tissue optics—in particular, the field of tissue diagnostics and imaging—that has occurred since 2007. As in the first two editions, Part I describes fundamentals and basic research, and Part II presents instrumentation and medical applications. However, for the reader’s convenience, this third edition has been reorganized into 14 chapters instead of 9. The chapters covering optical coherence tomography, digital holography and interferometry, controlling optical properties of tissues, nonlinear spectroscopy, and imaging have all been substantially updated. The book is intended for researchers, teachers, and graduate and undergraduate students specializing in the physics of living systems, biomedical optics and biophotonics, laser biophysics, and applications of lasers in biomedicine. It can also be used as a textbook for courses in medical physics, medical engineering, and medical biology.
High Resolution Imaging In Microscopy And Ophthalmology
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Author : Josef F. Bille
language : en
Publisher: Springer
Release Date : 2019-08-13
High Resolution Imaging In Microscopy And Ophthalmology written by Josef F. Bille and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Medical categories.
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Handbook Of Optical Biomedical Diagnostics
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Author : Valeriĭ Viktorovich Tuchin
language : en
Publisher:
Release Date : 2016
Handbook Of Optical Biomedical Diagnostics written by Valeriĭ Viktorovich Tuchin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Electronic books categories.
This text begins by describing the basic principles and diagnostic applications of optical techniques based on detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.
Handbook Of Optical Sensing Of Glucose In Biological Fluids And Tissues
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Author : Valery V. Tuchin
language : en
Publisher: CRC Press
Release Date : 2008-12-22
Handbook Of Optical Sensing Of Glucose In Biological Fluids And Tissues written by Valery V. Tuchin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-22 with Science categories.
Although noninvasive, continuous monitoring of glucose concentration in blood and tissues is one of the most challenging areas in medicine, a wide range of optical techniques has recently been designed to help develop robust noninvasive methods for glucose sensing. For the first time in book form, the Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues analyzes trends in noninvasive optical glucose sensing and discusses its impact on tissue optical properties. This handbook presents methods that improve the accuracy in glucose prediction based on infrared absorption spectroscopy, recent studies on the influence of acute hyperglycemia on cerebral blood flow, and the correlation between diabetes and the thermo-optical response of human skin. It examines skin glucose monitoring by near-infrared spectroscopy (NIR), fluorescence-based glucose biosensors, and a photonic crystal contact lens sensor. The contributors also explore problems of polarimetric glucose sensing in transparent and turbid tissues as well as offer a high-resolution optical technique for noninvasive, continuous, and accurate blood glucose monitoring and glucose diffusion measurement. Written by world-renowned experts in biomedical optics and biophotonics, this book gives a complete, state-of-the-art treatise on the design and applications of noninvasive optical methods and instruments for glucose sensing.
Confocal Microscopy
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Author : Stephen W. Paddock
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Confocal Microscopy written by Stephen W. Paddock and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.