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The Pcr Technique


The Pcr Technique
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Principles And Technical Aspects Of Pcr Amplification


Principles And Technical Aspects Of Pcr Amplification
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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14

Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.


Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).



Pcr


Pcr
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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-09-23

Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-23 with Science categories.


This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.



The Pcr Technique


The Pcr Technique
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Author : James W. Larrick
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1997

The Pcr Technique written by James W. Larrick and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.


A collection of papers originally appearing in BioTechniques, a journal of life science laboratory techniques, from 1991 to 1996, focusing on various aspects of quantitative PCR. Papers present novel methods to quantitate PCR products, improvements and modifications of previously published methods, and applications of quantitative PCR to a variety of clinical situations, especially viral analysis. Most papers are updated by their original authors at the conclusion of the original papers. As of February 1998, Books in Print erroneously shows the publisher to be Biotech Publishing, rather than Eaton Publishing. Annotation copyrighted by Book News, Inc., Portland, OR



Pcr Protocols


Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02

Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.


The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications



Pcr Technology


Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31

Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.


This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.



The Pcr Technique


The Pcr Technique
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Author : Paul D. Siebert
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1998

The Pcr Technique written by Paul D. Siebert and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.




The Pcr Technique


The Pcr Technique
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Author : Ulf Gyllensten
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1997

The Pcr Technique written by Ulf Gyllensten and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.




The Pcr Technique


The Pcr Technique
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Author : James Ellingboe
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1992

The Pcr Technique written by James Ellingboe and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA polymerases categories.




Pcr Technology


Pcr Technology
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Author : Tania Nolan
language : en
Publisher: CRC Press
Release Date : 2013-06-13

Pcr Technology written by Tania Nolan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-13 with Law categories.


PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.



Pcr Protocols


Pcr Protocols
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Author : John M. S. Bartlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Pcr Protocols written by John M. S. Bartlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.


In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.