Three Dimensional Electron Microscopy
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Three Dimensional Electron Microscopy
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Author :
language : en
Publisher: Academic Press
Release Date : 2019-07-18
Three Dimensional Electron Microscopy written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-18 with Science categories.
Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the Three-Dimensional Electron Microscopy technique
Three Dimensional Electron Microscopy Of Macromolecular Assemblies
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Author : Joachim Frank
language : en
Publisher: Oxford University Press
Release Date : 2006
Three Dimensional Electron Microscopy Of Macromolecular Assemblies written by Joachim Frank and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Computers categories.
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Three Dimensional Electron Microscopy Of Macromolecular Assemblies
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Author : Frank Joachim
language : en
Publisher: Elsevier
Release Date : 1996-01-24
Three Dimensional Electron Microscopy Of Macromolecular Assemblies written by Frank Joachim and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-01-24 with Science categories.
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods.Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins
Three Dimensional Electron Microscopy Of Macromolecular Assemblies
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Author : Joachim Frank
language : en
Publisher: Oxford University Press
Release Date : 2006-02-02
Three Dimensional Electron Microscopy Of Macromolecular Assemblies written by Joachim Frank and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-02 with Science categories.
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Electron Tomography
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Author : Joachim Frank
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Electron Tomography written by Joachim Frank and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.
Three Dimensional Electron Microscopy Of Macromolecular Assemblies
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Author : Joachim Frank
language : en
Publisher:
Release Date : 1996-01-01
Three Dimensional Electron Microscopy Of Macromolecular Assemblies written by Joachim Frank and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-01-01 with Science categories.
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
Computational Methods For Three Dimensional Microscopy Reconstruction
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Author : Gabor T. Herman
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-01-29
Computational Methods For Three Dimensional Microscopy Reconstruction written by Gabor T. Herman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-29 with Mathematics categories.
Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy and X-ray tomography can be captured in the same mathematical framework, leading to closely-related computational approaches, but the methodologies differ in detail and hence pose different challenges. The editors of this volume, Gabor T. Herman and Joachim Frank, are experts in the respective methodologies and present research at the forefront of biological imaging and structural biology. Computational Methods for Three-Dimensional Microscopy Reconstruction will serve as a useful resource for scholars interested in the development of computational methods for structural biology and cell biology, particularly in the area of 3D imaging and modeling.
Cryo Em Part B 3 D Reconstruction
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30
Cryo Em Part B 3 D Reconstruction written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Electron Tomography
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Author : Joachim Frank
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-05
Electron Tomography written by Joachim Frank and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-05 with Science categories.
This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
Cellular Electron Microscopy
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Author : J. Richard McIntosh
language : en
Publisher: Elsevier
Release Date : 2011-09-02
Cellular Electron Microscopy written by J. Richard McIntosh and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-02 with Science categories.
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. - Updates the numerous technological innovations that have improved the capabilities of electron microscopy - Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging - Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going