Using Mass Spectrometry For Biochemical Studies On Enzymatic Domains From Polyketide Synthases

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Using Mass Spectrometry For Biochemical Studies On Enzymatic Domains From Polyketide Synthases

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Author : Matthew Jenner
language : en
Publisher: Springer
Release Date : 2016-04-27

Using Mass Spectrometry For Biochemical Studies On Enzymatic Domains From Polyketide Synthases written by Matthew Jenner and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-27 with Science categories.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis. In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC. KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Biochemical Studies On Enzymatic Domains From Trans Acyltransferase Polyketide Synthases By Mass Spectrometry

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Author : Matthew Jenner
language : en
Publisher:
Release Date : 2015

Biochemical Studies On Enzymatic Domains From Trans Acyltransferase Polyketide Synthases By Mass Spectrometry written by Matthew Jenner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Polyketides categories.

Interactions Between Small Molecule Ligands And Target Enzymes

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Author : Sung-Kun (Sean) Kim
language : en
Publisher: Frontiers Media SA
Release Date : 2021-04-13

Interactions Between Small Molecule Ligands And Target Enzymes written by Sung-Kun (Sean) Kim and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-13 with Science categories.

Comprehensive Natural Products Iii

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Author :
language : en
Publisher: Elsevier
Release Date : 2020-07-22

Comprehensive Natural Products Iii written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-22 with Science categories.

Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience

The Search For Biological Active Agent S From Actinobacteria 2nd Edition

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Author : Learn-Han Lee
language : en
Publisher: Frontiers Media SA
Release Date : 2020-03-27

The Search For Biological Active Agent S From Actinobacteria 2nd Edition written by Learn-Han Lee and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-27 with categories.

There is a large market demand for new drugs. The existing chronic or common ailments without cures, development of new diseases with unknown causes, and the widespread existence of antibiotic-resistant pathogens, have driven this field of research further by looking at all potential sources of natural products. To date, microbes have made a significant contribution to the health and well-being of people globally. The discoveries of useful metabolites produced by microbes have resulted in a significant proportion of pharmaceutical products in today’s market. Therefore, the investigation and identification of bioactive compound(s) producing microbes is always of great interest to researchers. Actinobacteria are one of the most important and efficient groups of natural metabolite producers. Among the numerous genera, Streptomyces have been recognized as prolific producers of useful natural compounds, as they provide more than half of the naturally-occurring antibiotics isolated to-date and continue to emerge as the primary source of new bioactive compounds. Certainly, these potentials have attracted ample research interest and a wide range of biological activities have been subsequently screened by researchers with the utilization of different In vitro and In vivo model of experiments. Literature evidence has shown that a significant number of interesting compounds produced by Actinobacteria were exhibiting either strong anticancer or neuroprotective activity. The further in depth studies have then established the modulation of apoptotic pathway was involved in those observed bioactivities. These findings indirectly prove the biopharmaceutical potential possessed by Actinobacteria and at the same time substantiate the importance of diverse pharmaceutical evaluations on Actinobacteria. In fact, many novel compounds discovered from Actinobacteria with strong potential in clinical applications have been developed into new drugs by pharmaceutical companies. Together with the advancement in science and technology, it is predicted that there would be an expedition in discoveries of new bioactive compounds producing Actinobacteria from various sources, including soil and marine sources. In light of these current needs, and great interest in the scope of this research, this book seeks to contribute on the investigation of different biological active compound(s) producing actinobacteria which are exhibiting antimicrobial, antioxidant, neuroprotective, anticancer activities and similar.

Dissertation Abstracts International

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Author :
language : en
Publisher:
Release Date : 2008

Dissertation Abstracts International written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Dissertations, Academic categories.

Cumulated Index Medicus

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Author :
language : en
Publisher:
Release Date : 1999

Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medicine categories.

Engineering The Microbial Platform For The Production Of Biologics And Small Molecule Medicines

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Author : Dipesh Dhakal
language : en
Publisher: Frontiers Media SA
Release Date : 2019-12-23

Engineering The Microbial Platform For The Production Of Biologics And Small Molecule Medicines written by Dipesh Dhakal and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-23 with categories.

Index Medicus

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Author :
language : en
Publisher:
Release Date : 2004

Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medicine categories.

Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Activity Based Protein Profiling

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Author : Benjamin F. Cravatt
language : en
Publisher: Springer
Release Date : 2019-01-25

Activity Based Protein Profiling written by Benjamin F. Cravatt and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-25 with Medical categories.

This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.