Yeast Cell Surface Engineering
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Yeast Cell Surface Engineering
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Author : Mitsuyoshi Ueda
language : en
Publisher: Springer
Release Date : 2019-04-23
Yeast Cell Surface Engineering written by Mitsuyoshi Ueda and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-23 with Medical categories.
This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.
Cell Surface Engineering
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Author : Rawil Fakhrullin
language : en
Publisher: Royal Society of Chemistry
Release Date : 2014-07-24
Cell Surface Engineering written by Rawil Fakhrullin and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-24 with Medical categories.
A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.
Yeast Surface Display
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Author : Michael W. Traxlmayr
language : en
Publisher: Springer Nature
Release Date : 2022-04-28
Yeast Surface Display written by Michael W. Traxlmayr and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-28 with Science categories.
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Yeast Surface Display
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Author : Bin Liu
language : en
Publisher: Humana
Release Date : 2016-10-09
Yeast Surface Display written by Bin Liu and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-09 with Science categories.
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Utilization Of Yeast Pheromones And Hydrophobin Based Surface Engineering For Novel Whole Cell Sensor Applications
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Author : Stefan Hennig
language : en
Publisher:
Release Date : 2017
Utilization Of Yeast Pheromones And Hydrophobin Based Surface Engineering For Novel Whole Cell Sensor Applications written by Stefan Hennig and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Engineering An Improved Bioconjugation Enzyme
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Author : Anne Zijing Ye
language : en
Publisher:
Release Date : 2019
Engineering An Improved Bioconjugation Enzyme written by Anne Zijing Ye and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Enzyme engineering is a valuable tool for generating powerful and robust biocatalysts for a broad range of applications. Directed evolution using cell surface display has been previously applied for enzyme engineering, and involves iterative optimization of a protein sequence to yield a desired phenotype. However, previous applications have been carefully tailored for specific enzymes, and this process of developing a good screen is a significant bottleneck in engineering. Co-display of an enzyme and its substrate on the yeast surface is a promising general strategy for evolving bond-forming enzymes, but has only been used to engineer one enzyme. We extended yeast surface co-display to engineer Sfp, a bioconjugation enzyme, for improved activity with a non-native substrate. Sfp library screening identified mutational hotspots in the enzyme and led to the design of Sfp variants with orders of magnitude enhancements in activity. However, these activity improvements were only apparent in contexts similar to yeast surface co-display, reflecting artifacts in the use of this approach for enzyme engineering. Based on our results, we offer suggestions for improving the success of engineering strategies based on yeast surface display.
Yeast Metabolic Engineering
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Author : Valeria Mapelli
language : en
Publisher: Springer Nature
Release Date : 2022-07-04
Yeast Metabolic Engineering written by Valeria Mapelli and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-07-04 with Science categories.
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Materials Science And Engineering Concepts Methodologies Tools And Applications
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Author : Management Association, Information Resources
language : en
Publisher: IGI Global
Release Date : 2017-01-11
Materials Science And Engineering Concepts Methodologies Tools And Applications written by Management Association, Information Resources and has been published by IGI Global this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-11 with Technology & Engineering categories.
The design and study of materials is a pivotal component to new discoveries in the various fields of science and technology. By better understanding the components and structures of materials, researchers can increase its applications across different industries. Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications is a compendium of the latest academic material on investigations, technologies, and techniques pertaining to analyzing the synthesis and design of new materials. Through its broad and extensive coverage on a variety of crucial topics, such as nanomaterials, biomaterials, and relevant computational methods, this multi-volume work is an essential reference source for engineers, academics, researchers, students, professionals, and practitioners seeking innovative perspectives in the field of materials science and engineering.
Systemc From The Ground Up Second Edition
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Author : David C. Black
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-11-02
Systemc From The Ground Up Second Edition written by David C. Black and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-02 with Technology & Engineering categories.
SystemC provides a robust set of extensions to the C++ language that enables rapid development of complex models of hardware and software systems. The authors focus on practical use of the language for modeling real systems, showing: A step-by-step build-up of syntax Code examples for each concept Over 8000 lines of downloadable code examples Updates to reflect the SystemC standard, IEEE 1666 Why features are as they are Many resource references How SystemC fits into an ESL methodology This new edition of an industry best seller is updated to reflect the standardization of SystemC as IEEE 1666 and other improvements that reflect feedback from readers of the first edition. The wide ranging feedback also include suggestions from editors of the Japanese and Korean language translations, professors and students, and computer engineers from a broad industrial and geographical spectrum, all who have successfully used the first edition. New chapters have been added on the SystemC Verification Library and the Transaction Level Modeling, and proposed changes to the current SystemC standard. David Black and Jack Donovan, well known consultants in the EDA industry, have teamed with Bill Bunton and Anna Keist, experienced SystemC modeling engineers, to write the second edition of this highly popular classic. As a team the authors bring over 100 years of ASIC and system design experience together to make a very readable introduction to SystemC.
Biotechnology And Bioengineering
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Author : William G. Flynne
language : en
Publisher: Nova Publishers
Release Date : 2008
Biotechnology And Bioengineering written by William G. Flynne and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
Biotechnology is a popular term for the generic technology of the 21st century. Although it has been utilised for centuries in traditional production processes, modern biotechnology is only 50 years old and in the last decades it has been witnessing tremendous developments. Bioengineering is the science upon which all Biotechnological applications are based. With the development of new approaches and modern techniques, traditional biotechnology industries are also acquiring new horizons enabling them to improve the quality of their products and increase the productivity of their systems. Biological engineering (also biosystems engineering and bioengineering) deals with engineering biological processes in general. It is a broad-based engineering discipline that also may involve product design, sustainability and analysis of biological systems. In other words, Bioengineering is a discipline that applies engineering principles to biological systems for the purpose of developing new technologies of services to improve the living standards of societies. It exploits the new developments in molecular biology, biochemistry, microbiology, cell metabolism and engineering principles and applies them in order to understand living systems and to bring solutions various problems associated with these systems. This book presents leading research in both areas.