Yeast Metabolic Engineering
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Yeast Metabolic Engineering
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Author : Valeria Mapelli
language : en
Publisher: Springer Nature
Release Date : 2022-07-04
Yeast Metabolic Engineering written by Valeria Mapelli and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-07-04 with Science categories.
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Metabolic Engineering
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Author : Sang Yup Lee
language : en
Publisher: CRC Press
Release Date : 1999-09-29
Metabolic Engineering written by Sang Yup Lee and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-09-29 with Science categories.
This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.
Non Conventional Yeasts From Basic Research To Application
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Author : Andriy Sibirny
language : en
Publisher: Springer
Release Date : 2019-08-12
Non Conventional Yeasts From Basic Research To Application written by Andriy Sibirny and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-12 with Science categories.
This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Systems Metabolic Engineering
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Author : Christoph Wittmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-06-15
Systems Metabolic Engineering written by Christoph Wittmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-15 with Science categories.
Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.
An Introduction To Metabolic And Cellular Engineering
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Author : Sonia Cortassa
language : en
Publisher: World Scientific
Release Date : 2002
An Introduction To Metabolic And Cellular Engineering written by Sonia Cortassa and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Science categories.
Metabolic and cellular engineering is a powerful alliance of two technologies: genetics-molecular biology and fermentation technology.
Metabolic Engineering
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Author : Jens Nielsen
language : en
Publisher: Springer
Release Date : 2003-07-03
Metabolic Engineering written by Jens Nielsen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-03 with Science categories.
Metabolic engineering is a rapidly evolving field that is being applied for the optimization of many different industrial processes. In this issue of Advances in Biochemical Engineering/Biotechnology, developments in different areas of metabolic engineering are reviewed. The contributions discuss the application of metabolic engineering in the improvement of yield and productivity - illustrated by amino acid production and the production of novel compounds - in the production of polyketides and extension of the substrate range - and in the engineering of S. cerevisiae for xylose metabolism, and the improvement of a complex biotransformation process.
Microbial Biotechnology
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Author : Farshad Darvishi Harzevili
language : en
Publisher: CRC Press
Release Date : 2018-10-08
Microbial Biotechnology written by Farshad Darvishi Harzevili and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-08 with Medical categories.
Incorporates the Experiences of World-Class Researchers Microbial Biotechnology: Progress and Trends offers a theoretical take on topics that relate to microbial biotechnology. The text uses the "novel experimental experiences" of various contributors from around the world—designed as case studies—to highlight relevant topics, issues, and recent developments surrounding this highly interdisciplinary field. It factors in metagenomics and microbial biofuels production, and incorporates major contributions from a wide range of disciplines that include microbiology, biochemistry, genetics, molecular biology, chemistry, biochemical engineering, and bioprocess engineering. In addition, it also provides a variety of photos, diagrams, and tables to help illustrate the material. The book consists of 15 chapters and contains subject matter that addresses: Microbial biotechnology from its historical roots to its different processes Some of the new developments in upstream processes Solid-state fermentation as an interesting field in modern fermentation processes Recent developments in the production of valuable microbial products such as biofuels, organic acids, amino acids, probiotics, healthcare products, and edible biomass Important microbial activities such as biofertilizer, biocontrol, biodegradation, and bioremediation Students, scientists, and researchers can benefit from Microbial Biotechnology: Progress and Trends, a resource that addresses biotechnology, applied microbiology, bioprocess/fermentation technology, healthcare/pharmaceutical products, food innovations/food processing, plant agriculture/crop improvement, energy and environment management, and all disciplines related to microbial biotechnology.
Metabolic Engineering In Yeast
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Author : Vyron Lykiardopoulos
language : en
Publisher:
Release Date : 2002
Metabolic Engineering In Yeast written by Vyron Lykiardopoulos and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Biotechnology Of Yeasts And Filamentous Fungi
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Author : Andriy A. Sibirny
language : en
Publisher:
Release Date : 2025-02-18
Biotechnology Of Yeasts And Filamentous Fungi written by Andriy A. Sibirny and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-18 with Science categories.
Metabolic Engineering And Characterisation Of The Malolactic Wine Yeast Ml01
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Author : John Ivan Husnik
language : en
Publisher:
Release Date : 2007
Metabolic Engineering And Characterisation Of The Malolactic Wine Yeast Ml01 written by John Ivan Husnik and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Fermentation categories.
Malolactic fermentation (MLF) is essential for deacidification of high acid grape must and the production of well-balanced wines. The bacterial MLF is unreliable and stuck MLFs often lead to spoilage of wines and the production of biogenic amines. A genetically stable industrial strain of Saccharomyces cerevisiae was constructed by integrating a linear cassette containing the Schizosaccharomyces pombe malate permease gene (mae1) and the Oenococcus oeni malolactic gene (mleA) under control of the S. cerevisiae PGK1 promoter and terminator sequences into the URA3 locus of an industrial wine yeast strain. The malolactic yeast strain, ML01, completes the MLF during the alcoholic fermentation in a variety of musts including a high acid Chardonnay must containing 9.2 g/L of malate. ML01 cannot appreciably decarboxylate L-malic acid to L-lactic acid when present at levels below 1% of the total inoculum. ML01 contains no antibiotic resistance marker genes or vector DNA sequences. Global gene expression patterns and analysis of the proteome showed that no metabolic pathway was affected by the introduction of the malolactic cassette. The presence of the malolactic cassette in the genome does not affect growth, ethanol production, fermentation kinetics or metabolism of ML01. Wines produced by the ML01 yeast have lower volatile acidity and improved color properties compared to wines produced with the parental yeast and a bacterial MLF. GC/MS analysis of volatile compounds revealed that wine produced by ML01 did not contain any compounds that were not detected in wine produced with the parental strain S92 or with S92 and malolactic bacteria. Moreover, ML01 reduces the processing time after alcoholic fermentation and produces wine that is judged highest in overall quality by trained tasters. Analyses of the phenotype, DNA, RNA, and proteins demonstrate that the recombinant yeast ML01 is substantially equivalent to the parental strain S92. ML01 has been approved for use in Canada and has 'Generally Regarded As Safe' status with the US FDA. It is the first metabolically engineered yeast to be commercialised by the wine industry and is currently available in Canada, the USA and Moldova.