An Optimal Cell Detection Technique For Automated Patch Clamping
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An Optimal Cell Detection Technique For Automated Patch Clamping
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Author : National Aeronautics and Space Administration (NASA)
language : en
Publisher: Createspace Independent Publishing Platform
Release Date : 2018-06-21
An Optimal Cell Detection Technique For Automated Patch Clamping written by National Aeronautics and Space Administration (NASA) and has been published by Createspace Independent Publishing Platform this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-21 with categories.
While there are several hardware techniques for the automated patch clamping of cells that describe the equipment apparatus used for patch clamping, very few explain the science behind the actual technique of locating the ideal cell for a patch clamping procedure. We present a machine vision approach to patch clamping cell selection by developing an intelligent algorithm technique that gives the user the ability to determine the good cell to patch clamp in an image within one second. This technique will aid the user in determining the best candidates for patch clamping and will ultimately save time, increase efficiency and reduce cost. The ultimate goal is to combine intelligent processing with instrumentation and controls in order to produce a complete turnkey automated patch clamping system capable of accurately and reliably patch clamping cells with a minimum amount of human intervention. We present a unique technique that identifies good patch clamping cell candidates based on feature metrics of a cell's (x, y) position, major axis length, minor axis length, area, elongation, roundness, smoothness, angle of orientation, thinness and whether or not the cell is only particularly in the field of view. A patent is pending for this research. McDowell, Mark and Gray, Elizabeth Glenn Research Center NASA/TM-2004-213383, NAS/1.15:213383, E-14891
Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-06
Patch Clamp Analysis written by Wolfgang Walz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-06 with Medical categories.
Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Nasa Tech Briefs
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Author :
language : en
Publisher:
Release Date : 2008
Nasa Tech Briefs written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Technology categories.
Nanoscience
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Author : Patrick Boisseau
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-10-14
Nanoscience written by Patrick Boisseau and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-14 with Technology & Engineering categories.
Nanobiotechnology is a rapidly developing field of research with new applications constantly emerging. This book presents the basics, fundamental results and latest achievements of nanobiotechnological research. It extends as far as promising applications of new nanomaterials and newly discovered nanoeffects. Additionally, it presents a large variety of nanobio-analysis methods.
Patch Clamp Techniques
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Author : Yasunobu Okada
language : en
Publisher: Springer
Release Date : 2012-04-27
Patch Clamp Techniques written by Yasunobu Okada and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-27 with Medical categories.
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Gigaseal Formation In Patch Clamping
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Author : Majid Malboubi
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-07-24
Gigaseal Formation In Patch Clamping written by Majid Malboubi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-24 with Technology & Engineering categories.
This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
Ion Channel Regulation
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Author :
language : en
Publisher: Academic Press
Release Date : 1999-04-13
Ion Channel Regulation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-13 with Science categories.
Volume 33 reviews the current understanding of ion channel regulation by signal transduction pathways. Ion channels are no longer viewed simply as the voltage-gated resistors of biophysicists or the ligand-gated receptors of biochemists. They have been transformed during the past 20 years into signaling proteins that regulate every aspect of cell physiology. In addition to the voltage-gated channels, which provide the ionic currents to generate and spread neuronal activity, and the calcium ions to trigger synaptic transmission, hormonal secretion, and muscle contraction, new gene families of ion channel proteins regulate cell migration, cell cycle progression, apoptosis, and gene transcription, as well as electrical excitability. Even the genome of the lowly roundworm Caenorhabditis elegans encodes almost 100 distinct genes for potassium-selective channels alone. Most of these new channel proteins are insensitive to membrane potential, yet in humans, mutations in these genes disrupt development and increase individual susceptibility to debilitating and lethal diseases.How do cells regulate the activity of these channels? How might we restore their normal function? In Ion Channel Regulation, many of the experts who pioneered these discoveries provide detailed summaries of our current understanding of the molecular mechanisms that control ion channel activity. Reviews brain functioning at the fundamental, molecular level Describes key systems that control signaling between and within cells Explains how channels are used to stimulate growth and changes to activity of the nucleus and genome
Ion Channel Drug Discovery
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Author : Brian Cox
language : en
Publisher: Royal Society of Chemistry
Release Date : 2014-09-24
Ion Channel Drug Discovery written by Brian Cox and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-24 with Medical categories.
A rapidly growing field, this book covers the recent advances in screening technology, ion channel structure and modelling, with up-to-date case histories.
Glycinergic Transmission Physiological Developmental And Pathological Implications
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Author : Robert J. Harvey
language : en
Publisher: Frontiers E-books
Release Date :
Glycinergic Transmission Physiological Developmental And Pathological Implications written by Robert J. Harvey and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Inhibitory glycine receptors (GlyRs) containing the alpha1 and beta subunits are well known for their involvement in an inherited motor disorder (hyperekplexia) characterised by neonatal hypertonia and an exaggerated startle reflex. However, it has recently emerged that other GlyR subtypes (e.g. those containing the alpha2, alpha3 and alpha4 subunits) may play more diverse biological roles. New animal models of glycinergic dysfunction have been reported in zebrafish (bandoneon, shocked), mice (cincinatti, Nmf11) and cows (CMD2). In addition, key studies on neurotransmitter transporters for glycine (GlyT1, GlyT2, VIAAT) have also revealed key roles for these presynaptic and glial proteins in health and disease. Molecular modelling and structure/function studies have also provided key insights into allosteric signal transduction mechanisms and the diverse pharmacology of glycine receptors. This Research Topic aims to bring together experts in the field of glycinergic transmission, and invite research articles or topical reviews to provide an up-to-date perspective on the insights into receptor, transporter and synaptic function that can be gained by the study of glycinergic transmission.
Targeting Ion Channels For Drug Discovery Emerging Challenges For High Throughput Screening Technologies
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Author : Ciria Hernandez
language : en
Publisher: Frontiers Media SA
Release Date : 2024-06-07
Targeting Ion Channels For Drug Discovery Emerging Challenges For High Throughput Screening Technologies written by Ciria Hernandez and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-07 with Science categories.
Ligand and voltage-gated ion channels are highly regulated protein molecules that cross the cell membrane allowing ion flow from one side of the membrane to the other. They are ubiquitously expressed in human tissues and consist of one of the largest and best understood functional groups of proteins, with more than 400 members spanning nearly 1% of the human genome. They are involved in a variety of fundamental physiological processes, and their malfunction causes numerous diseases. In terms of the challenges faced in the effort to discover specific drugs in ancient and emerging diseases, ion channels are the third-largest class of target proteins after G-protein-coupled receptors (GPCRs) and kinases. 15% of small molecule drug targets have been reported to be voltage- or ligand-gated ion channels, resulting in approximately 150 new drug candidates in preclinical and clinical studies. Of the ion channel targeting drugs found on the market, these were identified more than a decade ago, and many of the current studies are at various stages of scientific approval. Overcoming these challenges has led the field of ion channel drug discovery to transform over the past 15 years through major advancements in genetic target detection, validation, structure-based drug design, and drug modeling of cell-based diseases.