Cell Structure And Function By Microspectrofluorometry
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Cell Structure And Function By Microspectrofluorometry
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Author : Elli Kohen
language : en
Publisher: Academic Press
Release Date : 2014-06-28
Cell Structure And Function By Microspectrofluorometry written by Elli Kohen and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Cell Structure and Function by Microspectrofluorometry provides an overview of the state of knowledge in the study of cellular structure and function using microspectrofluorometry. The book is organized into six parts. Part I begins by tracing the origins of modern fluorescence microscopy and fluorescent probes. Part II discusses methods such as microspectroscopy and flow cytometry; the fluorescence spectroscopy of solutions; and the quantitative implementation of fluorescence resonance energy transfer (FRET) in the light microscope. Part III presents studies on metabolism, including the mechanism of action of xenobiotics; biochemical analysis of unpigmented single cells; and cell-to-cell communication in the endocrine and the exocrine pancreas. Part IV focuses on applications of fluorescent probes. Part V deals with cytometry and cell sorting. It includes studies on principles and characteristics of flow cytometry as a method for studying receptor-mediated endocytosis; and flow cytometric measurements of physiologic cell responses. Part VI on bioluminescence discusses approaches to measuring chemiluminescence or bioluminescence in a single cell and measuring light emitted by living cells.
Cell Structure And Function By Microspectrofluorometry
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Author : Elli Kohen
language : en
Publisher:
Release Date : 1989-01-01
Cell Structure And Function By Microspectrofluorometry written by Elli Kohen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-01-01 with Science categories.
Cell Structure and Function by Microspectrofluorometry
Molecular And Cellular Enzymology
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Author : P. Jeanteur
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Molecular And Cellular Enzymology written by P. Jeanteur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Proteins constitute the working-class molecules of the cell. Hence, understanding the way they act is a prerequisite for understanding how a cell functions and how life evolves. Aspects such as the protein-ligand relationship, recognition, protein evolution by point mutation, enzyme-substrate interactions, behaviour of an enzyme in a living cell, control and dynamics of enzyme networks as well as the physico-chemical background of enzyme actions and multi-enzyme complexes are comprehensively treated in this volume.
Surgical Research
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Author : Wiley W. Souba
language : en
Publisher: Academic Press
Release Date : 2001-01-25
Surgical Research written by Wiley W. Souba and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-01-25 with Health & Fitness categories.
Contributors. -- Foreword. -- Preface. -- Getting Started. -- Assessing Available Information. -- Organizing and Preliminary Planning for Surgical Research -- Writing a Protocol: Animals, Humans, and Use of Biologic, Chemical, and Radiologic Agents. -- Grantsmanship. -- Informed Consent and the Protection of Human Research Subjects: Historical Perspectives and Guide to Current United States Regulations. -- Animal Care and Maintenance. -- Funding Strategies and Agencies: Academic-Industrial Relationships; Intellectual Property. -- Statistical Considerations. -- Use of Nonexperimental Studies to Evaluate Surgical Procedures and Other Interventions: The Challenge of Risk Adjustment. -- Measuring Surgical Outcomes. -- Design of Clinical Trials. -- Using Administrative Data for Clinical Research. -- Research in the Intensive Care Unit: Ethical and Methodological Issues. -- Research in the Operating Room. -- Effects of Age and Gender. -- Strategies, Principles, and Techniques Using Transgeni ...
Femtosecond Biophotonics
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Author : Min Gu
language : en
Publisher: Cambridge University Press
Release Date : 2010-05-06
Femtosecond Biophotonics written by Min Gu and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-06 with Medical categories.
Covering key techniques for optical microscopy and micro-fabrication, this book provides the first detailed treatment of femtosecond laser-based biophotonics. After a review of the techniques for nonlinear and multiphoton imaging, applications for laser-based manipulation of micro-particles are introduced. The final chapter focuses on the burgeoning field of femtosecond micro-engineering.
Chlorophyll A Fluorescence
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Author : G.C. Papageorgiou
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-12
Chlorophyll A Fluorescence written by G.C. Papageorgiou and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-12 with Science categories.
Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture.
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. - Volume investigation - Three-dimensional reconstruction - Fluroescent probe design - Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology - Confocal data display and analysis - Twenty-eight pages of color
Fret And Flim Techniques
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Author : Theodorus W. J. Gadella
language : en
Publisher: Elsevier
Release Date : 2011-07-29
Fret And Flim Techniques written by Theodorus W. J. Gadella and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-29 with Science categories.
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Conn S Biological Stains
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Author : Richard Horobin
language : en
Publisher: Taylor & Francis
Release Date : 2020-08-13
Conn S Biological Stains written by Richard Horobin and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-13 with Science categories.
Published on behalf of the Biological Stain Commission For 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application. This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.
Photobiology
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Author : Elli Kohen
language : en
Publisher: Elsevier
Release Date : 1995-08-16
Photobiology written by Elli Kohen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-08-16 with Science categories.
Photobiology integrates a wide variety of scientific disciplines. As more people become aware of the many ways light interacts with chemical and biological systems, the need for a concise treatment of photobiology has become more critical. Kohen et al. Have written just such a book, intended both as a textbook and as a reference.The authors begin by providing a brief description of the nature of light, how it affects matter, and the means and methods of measuring it. A major section of the book is devoted to how light influences living systems, including discussions of photosynthesis, bioluminescence, regulatory mechanisms, and visual transduction of light. The last half of the book is devoted to the biomedical aspects of light, including photoimmunology, photoallergic reactions and other forms of light sensitivity, the optical properties of skin, and various ways that light can be used in therapy treatments.Useful to photobiologists as a comprehensive overview, this book should also appeal to biomedical researchers and advanced students of photobiology.