Fret And Flim Techniques
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Fret And Flim Techniques
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Author : Theodorus W. J. Gadella
language : en
Publisher: Elsevier
Release Date : 2011-07-29
Fret And Flim Techniques written by Theodorus W. J. Gadella and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-29 with Science categories.
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-05-06
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-06 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Microscopy Techniques
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Author : Jens Rietdorf
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-06-23
Microscopy Techniques written by Jens Rietdorf and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-06-23 with Science categories.
With contributions by numerous experts
Methods In Cellular Imaging
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Author : Ammasi Periasamy
language : en
Publisher: Springer
Release Date : 2013-05-27
Methods In Cellular Imaging written by Ammasi Periasamy and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-27 with Science categories.
Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Time Correlated Single Photon Counting
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Author : Desmond O'Connor
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Time Correlated Single Photon Counting written by Desmond O'Connor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.
Fret F Rster Resonance Energy Transfer
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Author : Igor L. Medintz
language : en
Publisher: John Wiley & Sons
Release Date : 2013-10-17
Fret F Rster Resonance Energy Transfer written by Igor L. Medintz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-17 with Science categories.
FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.
Advanced Time Correlated Single Photon Counting Techniques
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Author : Wolfgang Becker
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-12-19
Advanced Time Correlated Single Photon Counting Techniques written by Wolfgang Becker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-19 with Science categories.
In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.
Flim Microscopy In Biology And Medicine
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Author : Ammasi Periasamy
language : en
Publisher: CRC Press
Release Date : 2009-07-06
Flim Microscopy In Biology And Medicine written by Ammasi Periasamy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-06 with Science categories.
Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be
Fluorescence Lifetime Imaging Ophthalmoscopy
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Author : Martin Zinkernagel
language : en
Publisher: Springer
Release Date : 2019-07-29
Fluorescence Lifetime Imaging Ophthalmoscopy written by Martin Zinkernagel and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-29 with Medical categories.
This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.
Biochemical And Biophysical Methods In Molecular And Cellular Biology
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Author : Umakanta Tripathy
language : en
Publisher: Springer Nature
Release Date : 2025-03-10
Biochemical And Biophysical Methods In Molecular And Cellular Biology written by Umakanta Tripathy and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-10 with Science categories.
This book focuses on the fundamental principles and applications of several modern biochemical and biophysical techniques employed in molecular and cellular biology. It describes cutting-edge techniques for studying single molecules/biomolecules, subcellular structures, and cells. The book chapters provide an in-depth understanding of methods currently employed to visualize and probe molecular and cellular processes. The techniques discussed in this book include Mass spectrometry, Microscopy techniques, Forster resonance energy transfer (FRET), Z-scan, Fluorescence correlation and cross-correlation spectroscopy, Dynamic light scattering (DLS), X-ray crystallography, Total internal reflection fluorescence (TIRF) microscopy, Cryo-EM, NMR spectroscopy, Optical tweezers, Magnetic tweezers, Raman spectroscopy, Atomic force microscopy (AFM), Optogenetics, bioinformatics, etc. The book chapters also include the biomedical, industrial, and R&D applications of these methods. Also included are sections on data analysis and its interpretation. Overall, this book offers a comprehensive and detailed understanding of several modern techniques in molecular and cellular biology.