Flim Microscopy In Biology And Medicine
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Flim Microscopy In Biology And Medicine
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Author : Ammasi Periasamy
language : en
Publisher: CRC Press
Release Date : 2009-07-06
Flim Microscopy In Biology And Medicine written by Ammasi Periasamy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-06 with Science categories.
Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be
Multiphoton Microscopy And Fluorescence Lifetime Imaging
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Author : Karsten König
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2018-01-22
Multiphoton Microscopy And Fluorescence Lifetime Imaging written by Karsten König and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-22 with Science categories.
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography
Multiphoton Microscopy And Fluorescence Lifetime Imaging
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Author : Karsten König
language : en
Publisher:
Release Date : 2016
Multiphoton Microscopy And Fluorescence Lifetime Imaging written by Karsten König and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Flourescence Microscopy
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Author : Brian Herman
language : en
Publisher: Springer
Release Date : 1998
Flourescence Microscopy written by Brian Herman and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
The fluorescence microscope is an important biological laboratory tool, used to detect dells or molecules that emit fluorescence. This book deals with the fundamental principles and techniques, covering topics such as immunofluorescence, in-situ hybridization, and fluorescence photomicrography. In addition, a complete troubleshooting guide with practical hints is provided. 60 illus.
Fret And Flim Techniques
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Author : Theodorus W. J. Gadella
language : en
Publisher: Elsevier
Release Date : 2011-07-29
Fret And Flim Techniques written by Theodorus W. J. Gadella and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-29 with Science categories.
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Digital Microscopy
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Author : Greenfield Sluder
language : en
Publisher: Elsevier
Release Date : 2007-04-26
Digital Microscopy written by Greenfield Sluder and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-04-26 with Science categories.
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
Fluorescence Microscopy
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Author : B. Herman
language : en
Publisher: Taylor & Francis
Release Date : 2020-08-26
Fluorescence Microscopy written by B. Herman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-26 with Science categories.
This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.
Advanced Time Correlated Single Photon Counting Applications
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Author : Wolfgang Becker
language : en
Publisher: Springer
Release Date : 2015-04-13
Advanced Time Correlated Single Photon Counting Applications written by Wolfgang Becker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-13 with Science categories.
This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.
Advances In Brain Imaging Techniques
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Author : Nirmal Mazumder
language : en
Publisher: Springer Nature
Release Date : 2022-05-19
Advances In Brain Imaging Techniques written by Nirmal Mazumder and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-19 with Medical categories.
The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.