Cryo Electron Tomography
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Cryo Electron Tomography
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Author : Friedrich Förster
language : en
Publisher: Springer Nature
Release Date :
Cryo Electron Tomography written by Friedrich Förster and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Cellular Imaging
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Author : Eric Hanssen
language : en
Publisher: Springer
Release Date : 2017-12-30
Cellular Imaging written by Eric Hanssen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Science categories.
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Electron Tomography
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Author : Joachim Frank
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-05
Electron Tomography written by Joachim Frank and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-05 with Science categories.
This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
Cryo Em Part C
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30
Cryo Em Part C written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.
This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. Includes time-tested core methods and new innovations applicable to any researcher Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide
Handbook Of Cryo Preparation Methods For Electron Microscopy
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Author : Annie Cavalier
language : en
Publisher: CRC Press
Release Date : 2008-10-02
Handbook Of Cryo Preparation Methods For Electron Microscopy written by Annie Cavalier and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-10-02 with Science categories.
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook – Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis Ö Teach how to arrest physiological processes by cryo-fixation Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling Ö Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.
Computational And Experimental Techniques For Expanding The Applicability Of Cryo Electron Tomography To Cell Biology
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Author : Matthew Croxford
language : en
Publisher:
Release Date : 2022
Computational And Experimental Techniques For Expanding The Applicability Of Cryo Electron Tomography To Cell Biology written by Matthew Croxford and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
The field of cell biology originated with, and has always been inextricably tied to, the microscope. Direct visualization has always been a key technique for understanding cell organization and behavior. Today, cell biologists have microscopes many orders of magnitude more powerful than their progenitors, enabling the direct visualization of cells and their constituent parts down to sub-nanometer resolution. Cryo-focused ion beam milling combined with cryo-elecyron tomography (cryo-FIB-ET) is a technique in which cells are rapidly frozen to preserve them in their native state, followed by focused ion beam (FIB) thinning of the frozen cells, which allows for the collection of high resolution transmission electron microscopy data. When these electron micrographs are collected over a range of different tilt angles, a full three-dimensional reconstruction of the cellular interior can be made and analyzed. However, despite the unprecedented level of detail available to cell biologists through cryo-FIB-ET, several challenges hinder its applicability to cell biology. On the experimental side, commonly used cryo-FIB-ET compatible substrates were not designed with cell biology in mind, and have features that make them sub-optimal for this purpose. Furthermore, limitations in the range of tilts at which data may be collected lead to missing information in the three-dimensional reconstructions that cause distortions and make the resulting volumes more difficult to interpret. The work presented in this dissertation focuses on both of these issues in an attempt to increase the utility of cryo-FIB-ET for cell biologists and expand the range of questions that may be addressed with the technique.
Cryo Electron Tomography
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Author : Dorit Hanein
language : en
Publisher: Elsevier
Release Date : 2024-12-01
Cryo Electron Tomography written by Dorit Hanein and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-12-01 with Science categories.
Cryo-electron Tomography: A Journey from Sample Preparation to Data Mining offers a comprehensive guide to the latest state-of-the-art methods and techniques available to researchers. The book begins with a section on sample preparation, considering a range of different sample types including virus-like particles, viruses, bacteria, organoids and tissues, with some chapters dedicated to specific tools like in situ cellular tomography and correlative techniques. The book then moves on to a section on data mining and specific steps and methods, delivering a complete overview of this advancing technique and providing researchers with the knowledge and tools required for their own investigations. Provides a detailed overview of cryo-ET sample preparation and data mining protocols and approaches in a single resource Includes foundational knowledge underpinning the field Explores the latest methods, techniques and recent developments in cryo-ET Offers step-by-step guidance to researchers for key procedures Written by an international range of experts
Development And Application Of Cryo Electron Tomography Technologies For Neuronal Cell Biology
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Author : Joseph Yoojong Kim
language : en
Publisher:
Release Date : 2024
Development And Application Of Cryo Electron Tomography Technologies For Neuronal Cell Biology written by Joseph Yoojong Kim and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024 with categories.
Fundamental understanding of neurons, the cellular unit of the central and peripheral nervous system, have been advanced by the advent of new microscopy techniques. In the past 20 years, cryo-electron tomography (cryo-ET) has emerged as a potent electron microscopy methodology to study neurons at nanometer or sub-nanometer resolution. By growing primary neuronal cultures on electron microscopy (EM) grids and rapidly freezing them in liquid ethane, they will be preserved in a near-native, hydrated state amenable for cryo-electron microscopy (cryo-EM). Tilted images (tilt series) can be acquired on thin cellular regions to reconstruct into a 3D volume known as a tomogram for 3D visualization. Such tomograms have provided new neurobiological insights such as cytoskeletal architecture and receptor organization at the postsynaptic membrane. However, neuronal cryo-ET is still a nascent field that can benefit from improvements in technique and new applications. In this thesis, I will describe my efforts in developing new cryo-ET technologies for neuronal cell biology such as micropatterning EM grids and montage tilt series acquisition. I will then detail applying these technologies to establish a new neuronal model from the common fruit fly, Drosophila melanogaster, while simultaneously discovering difficulties in cellular cryo-ET sample preparation that have not been previously recognized by the field.. Finally, I will present preliminary results on utilizing cryo-ET and auxiliary techniques for new ultrastructural effects on modulating proteins involved in neurite initiation (known as neuritogenesis) and attempt to utilize a dual-fluorescent transgenic Drosophila model to distinguish between axons and dendrites at a near-native, nanometer-level resolution.
Cryo Electron Microscopy In Structural Biology
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Author : Krishnarao Appasani
language : en
Publisher: CRC Press
Release Date : 2024-10-11
Cryo Electron Microscopy In Structural Biology written by Krishnarao Appasani and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-11 with Juvenile Nonfiction categories.
Cryo-Electron Microscopy, in combination with Tomography, has emerged as a new technology for visualizing molecular structures revealing the native molecular details of membranes, viruses, and cells. This comprehensive, volume brings together authoritative overviews of these methods from structural and biological perspective.
Computational Methods For Three Dimensional Microscopy Reconstruction
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Author : Gabor T. Herman
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-01-29
Computational Methods For Three Dimensional Microscopy Reconstruction written by Gabor T. Herman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-29 with Mathematics categories.
Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy and X-ray tomography can be captured in the same mathematical framework, leading to closely-related computational approaches, but the methodologies differ in detail and hence pose different challenges. The editors of this volume, Gabor T. Herman and Joachim Frank, are experts in the respective methodologies and present research at the forefront of biological imaging and structural biology. Computational Methods for Three-Dimensional Microscopy Reconstruction will serve as a useful resource for scholars interested in the development of computational methods for structural biology and cell biology, particularly in the area of 3D imaging and modeling.