Handbook Of Cryo Preparation Methods For Electron Microscopy
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Handbook Of Cryo Preparation Methods For Electron Microscopy
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Author : Annie Cavalier
language : en
Publisher: CRC Press
Release Date : 2008-10-02
Handbook Of Cryo Preparation Methods For Electron Microscopy written by Annie Cavalier and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-10-02 with Science categories.
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook – Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis Ö Teach how to arrest physiological processes by cryo-fixation Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling Ö Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.
Sample Preparation Handbook For Transmission Electron Microscopy
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Author : Jeanne Ayache
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-07-03
Sample Preparation Handbook For Transmission Electron Microscopy written by Jeanne Ayache and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-03 with Technology & Engineering categories.
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Electron Microscopy Of Model Systems
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-24
Electron Microscopy Of Model Systems written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-24 with Science categories.
The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. - Covers the preparation and analysis of model systems for biological electron microscopy - Includes the most popular systems but also organisms that are less frequently used in cell biology - Presents the currently most important methods for the preparation of biological specimens - First compendium covering the various aspects of sample preparation of very diverse biological systems
Biological Field Emission Scanning Electron Microscopy 2 Volume Set
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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-29
Biological Field Emission Scanning Electron Microscopy 2 Volume Set written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-29 with Science categories.
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Springer Handbook Of Microscopy
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Author : Peter W. Hawkes
language : en
Publisher: Springer Nature
Release Date : 2019-11-02
Springer Handbook Of Microscopy written by Peter W. Hawkes and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-02 with Technology & Engineering categories.
This book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology. Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.
Sample Preparation Handbook For Transmission Electron Microscopy
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Author : Jeanne Ayache
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-06-08
Sample Preparation Handbook For Transmission Electron Microscopy written by Jeanne Ayache and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-06-08 with Technology & Engineering categories.
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Cryo Em Part A Sample Preparation And Data Collection
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30
Cryo Em Part A Sample Preparation And Data Collection written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.
Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Encyclopedia Of Cell Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 2015-08-07
Encyclopedia Of Cell Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-07 with Science categories.
The Encyclopedia of Cell Biology, Four Volume Set offers a broad overview of cell biology, offering reputable, foundational content for researchers and students across the biological and medical sciences. This important work includes 285 articles from domain experts covering every aspect of cell biology, with fully annotated figures, abundant illustrations, videos, and references for further reading. Each entry is built with a layered approach to the content, providing basic information for those new to the area and more detailed material for the more experienced researcher. With authored contributions by experts in the field, the Encyclopedia of Cell Biology provides a fully cross-referenced, one-stop resource for students, researchers, and teaching faculty across the biological and medical sciences. Fully annotated color images and videos for full comprehension of concepts, with layered content for readers from different levels of experience Includes information on cytokinesis, cell biology, cell mechanics, cytoskeleton dynamics, stem cells, prokaryotic cell biology, RNA biology, aging, cell growth, cell Injury, and more In-depth linking to Academic Press/Elsevier content and additional links to outside websites and resources for further reading A one-stop resource for students, researchers, and teaching faculty across the biological and medical sciences
Molecular Gels
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Author : Richard G Weiss
language : en
Publisher: Royal Society of Chemistry
Release Date : 2018-08-06
Molecular Gels written by Richard G Weiss and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-06 with Science categories.
Over the past decade, research on molecular gels has expanded and progressed rapidly. This timely book presents the latest developments and understanding of the topic, covering the different classes of gels (ionogels, metallogels, hydrogels and organogels) from their molecular level interactions and formation to their properties as materials and applications. The book starts with a general introduction to molecular gels including their definition and a comparison of the different types. Chapters then examine the physical chemistry of molecular gels, including the most recent theories, experimental techniques and computational approaches. Final chapters on the applications of molecular gels illustrate, with modern case studies, the principles developed in previous chapters. Written by leading researchers, this is an indispensable resource on the different types of gels for postgraduate students and researchers across supramolecular chemistry, materials science, polymer chemistry, soft matter and chemical engineering.
Techniques For Work With Plant And Soil Nematodes
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Author : Roland N. Perry
language : en
Publisher: CABI
Release Date : 2020-11-26
Techniques For Work With Plant And Soil Nematodes written by Roland N. Perry and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-26 with Science categories.
Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included.