Detection Of Non Amplified Genomic Dna
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Detection Of Non Amplified Genomic Dna
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Author : Giuseppe Spoto
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-06
Detection Of Non Amplified Genomic Dna written by Giuseppe Spoto and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-06 with Science categories.
This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.
Dna Amplification
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Author : Vadim V. Demidov
language : en
Publisher: Taylor & Francis
Release Date : 2004
Dna Amplification written by Vadim V. Demidov and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Whole Genome Amplification
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Author : Simon Hughes
language : en
Publisher: Scion Publishing Ltd
Release Date : 2005-09-01
Whole Genome Amplification written by Simon Hughes and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-09-01 with Science categories.
Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Express is a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.
Pcr Mutation Detection Protocols
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Author : Bimal D. M. Theophilus
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Pcr Mutation Detection Protocols written by Bimal D. M. Theophilus and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Fingerprinting Methods Based On Arbitrarily Primed Pcr
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Author : Maria R. Micheli
language : en
Publisher: Springer
Release Date : 1997-01-29
Fingerprinting Methods Based On Arbitrarily Primed Pcr written by Maria R. Micheli and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-01-29 with Law categories.
Before the polymerase chain resction (PCR) was established,DNA fingerprinting technology had relied for years on restriction fragment length polymorphism (RFLP) analysis,a very efficient technique but quite laborious and not suitable for high throughput applications.Since its development,PCR has provided a new and powerful tool for DNA fingerprinting.
Molecular Techniques In Taxonomy
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Author : Godfrey M. Hewitt
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Molecular Techniques In Taxonomy written by Godfrey M. Hewitt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
Laboratory Methods For The Detection Of Mutations And Polymorphisms In Dna
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Author : Graham R. Taylor
language : en
Publisher: CRC Press
Release Date : 1997-01-24
Laboratory Methods For The Detection Of Mutations And Polymorphisms In Dna written by Graham R. Taylor and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-01-24 with Science categories.
The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Nonhuman Dna Typing
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Author : Heather Miller Coyle
language : en
Publisher: CRC Press
Release Date : 2007-08-29
Nonhuman Dna Typing written by Heather Miller Coyle and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-29 with Law categories.
The association of a suspect with the victim or crime scene through DNA evidence is one of the most powerful statements of complicity in a crime imaginable. No category of evidence has ever had the complete capacity to convict or exonerate an accused so absolutely in the eyes of the public. With the discriminatory powers of DNA and the variety of D
The Polymerase Chain Reaction
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Author : Kary B. Mullis
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-02-02
The Polymerase Chain Reaction written by Kary B. Mullis and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-02 with Medical categories.
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Molecular Diagnostics
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Author : William B. Coleman
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-18
Molecular Diagnostics written by William B. Coleman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-18 with Medical categories.
Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.