Fingerprinting Methods Based On Arbitrarily Primed Pcr
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Fingerprinting Methods Based On Arbitrarily Primed Pcr
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Author : Maria R. Micheli
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fingerprinting Methods Based On Arbitrarily Primed Pcr written by Maria R. Micheli and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.
Bacterial Genomes
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Author : F.J. de Bruijn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Bacterial Genomes written by F.J. de Bruijn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
A wide range of microbiologists, molecular biologists, and molecular evolutionary biologists will find this new volume of singular interest. It summarizes the present knowledge about the structure and stability of microbial genomes, and reviews the techniques used to analyze and fingerprint them. Maps of approximately thirty important microbes, along with articles on the construction and relevant features of the maps are included. The volume is not intended as a complete compendium of all information on microbial genomes, but rather focuses on approaches, methods and good examples of the analysis of small genomes.
Pcr In Bioanalysis
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Author : Stephen J Meltzer
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr In Bioanalysis written by Stephen J Meltzer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Dna Fingerprinting In Plants
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Author : Kurt Weising
language : en
Publisher: CRC Press
Release Date : 2005-02-28
Dna Fingerprinting In Plants written by Kurt Weising and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-28 with Science categories.
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p
Analytical Molecular Biology
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Author : Helen Parkes
language : en
Publisher: Royal Society of Chemistry
Release Date : 2007-10-31
Analytical Molecular Biology written by Helen Parkes and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-31 with Science categories.
In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement) Programme, introduces the issues of validation and quality to the bioanalytical community, specifically addressing DNA-based analyses. It aims to raise awareness of the factors that can influence the validity of DNA analysis and the production of quality data. Emphasis is placed on VAM principles, as well as additional challenges that are associated with the analysis of real samples, for example, complex food matrices or forensic samples that have been subjected to environmental insult. Information is collated from a variety of sources including literature, discussions and LGC research, and offers constructive advice where possible.
Dna Based Markers In Plants
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Author : R.L. Phillips
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14
Dna Based Markers In Plants written by R.L. Phillips and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Science categories.
With the new techniques described in this volume, a new gene can be placed on the linkage map within only a few days. Leading researchers have updated the earlier edition to include the latest versions of DNA-based marker maps for a variety of important crops.
Laboratory Methods For The Detection Of Mutations And Polymorphisms In Dna
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Author : Graham R. Taylor
language : en
Publisher: CRC Press
Release Date : 1997-01-24
Laboratory Methods For The Detection Of Mutations And Polymorphisms In Dna written by Graham R. Taylor and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-01-24 with Science categories.
The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Molecular Microbial Ecology
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Author : Mark Osborn
language : en
Publisher: Garland Science
Release Date : 2004-03-01
Molecular Microbial Ecology written by Mark Osborn and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.
Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.
Pcr
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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-09-23
Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-23 with Science categories.
This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Manual Of Commercial Methods In Clinical Microbiology
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Author : Allan L. Truant
language : en
Publisher: American Society for Microbiology Press
Release Date : 2001-12-18
Manual Of Commercial Methods In Clinical Microbiology written by Allan L. Truant and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-12-18 with Medical categories.
A general resource for all subdisciplines of clinical microbiology to use when evaluating commercial methods, tests, or procedures. • Reviews all the commercially available tests (both manual and automated) in the discipline of clinical microbiology. • Includes a description of the sensitivities, specificities, and predictive values from peer-reviewed sources. • Features separate chapters devoted to molecular microbiology, information management, emerging infectious diseases, and veterinary clinical microbiology.