Dna Polymerases In Biotechnology
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Dna Polymerases In Biotechnology
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Author : Zvi Kelman
language : en
Publisher: Frontiers Media SA
Release Date : 2015-03-18
Dna Polymerases In Biotechnology written by Zvi Kelman and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-03-18 with Biotechnology categories.
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Human Dna Polymerases Biology Medicine And Biotechnology
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Author : Maga Giovanni
language : en
Publisher: World Scientific
Release Date : 2017-11-10
Human Dna Polymerases Biology Medicine And Biotechnology written by Maga Giovanni and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-10 with Science categories.
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes. Contents: PrefaceAbout the AuthorsHistory of DNA PolymerasesDNA Polymerases: General AspectsHuman DNA Polymerases: From Structure to FunctionHuman DNA Polymerases in Different DNA TransactionsDNA Polymerases and Human DiseasesHuman DNA Polymerases and ChemotherapyPolymerases Chain Reaction and Heat-Stable DNA Polymerases: The History and the Potential of Evolved DNA PolymerasesSynthetic Evolution of DNA Polymerases for Novel PropertiesMarket for Evolved DNA Polymerases in Routine and Medical Applications Readership: Academic and industry research scientists, from PhD students to senior professors, as well as R&D specialists and marketing experts working in biotech and pharmaceutical companies. Keywords: DNA Polymerase;DNA Replication;DNA Repair;DNA Recombination, PCR;Cancer;Neurological Diseases;Medicine;Biology;Chemotherapy;Structural Biology;Enzymology;BiotechnologyReview: Key Features: The only book to merge basic science, biotechnological applications and marketing opportunities of DNA polymerasesThe most extensive literature coverage of the field, with more than 1,000 cited references and updated with the most recent contributions received by scientists all over the worldWritten by four leading experts in DNA polymerases, it gives the most complete overview of the field from its historical origins to the latest developments
Dna Polymerases
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Author :
language : en
Publisher: World Scientific
Release Date : 2010
Dna Polymerases written by and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with DNA polymerases categories.
Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Palgrave Macmillan
Release Date : 1989-10-01
Pcr Technology written by Henry Erlich and has been published by Palgrave Macmillan this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-10-01 with Science categories.
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31
Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Enzymology Primer For Recombinant Dna Technology
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Author : Hyone-Myong Eun
language : en
Publisher: Elsevier
Release Date : 1996-06-03
Enzymology Primer For Recombinant Dna Technology written by Hyone-Myong Eun and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-03 with Science categories.
Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Dna Amplification
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Author : Vadim V. Demidov
language : en
Publisher: Taylor & Francis
Release Date : 2004
Dna Amplification written by Vadim V. Demidov and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Pcr
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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-09-23
Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-23 with Science categories.
This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Polymerase Chain Reaction
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Author : Patricia Hernandez-Rodriguez
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-05-30
Polymerase Chain Reaction written by Patricia Hernandez-Rodriguez and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-30 with Science categories.
This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.
Pcr Technology
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Author : Henry A. Erlich
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Pcr Technology written by Henry A. Erlich and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA categories.
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.