Effective Regulation Of Peptide Hormones
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Effective Regulation Of Peptide Hormones
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Author : Feihua Cao
language : en
Publisher:
Release Date : 2012
Effective Regulation Of Peptide Hormones written by Feihua Cao and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Peptide hormones categories.
In the work described in this thesis, peptidylglycine a-amidating monooxygenase (PAM) was extracted from human H889 and DMS53 small cell lung cancer (SCLC) cells, PC-3 prostate cancer cells and SW1783 glioblastoma cells. Optimization of PAM assay conditions was carried out to maximize PAM activity. The extracted enzymes and optimized assay conditions provided a platform to assess substrate and inhibitor kinetic parameters and to compare the enzyme specificities of PAM from different human cancer sources, as well as differences between human PAM and commercial frog PAM. Potent and selective PAM inhibitors were then developed on the basis of structure-activity-relationships as well as the recognition by PAM of the peptide chain of natural substrates. Furthermore, a direct, rapid, efficient and sensitive HPLC method was developed in order to carry out whole cell inhibition studies and to directly monitor the inhibitory effects on natural hormone and prohormone levels. This method includes online extraction, separation, derivatization and fluorescence detection of peptide hormones, and in principle could be used for any peptide hormone with a free primary amine for fluorescamine tagging. Calcitonin (CT) and its prohormones were studied due to their high expression in DMS53 cells. This is the first time that the levels of CT and its prohormones, CTG, CTGK and CTGKK, have been separated and quantified separately. The detection limits of the present HPLC method were determined to be around 1 ng/mL of cell medium. This method was then applied to whole cell inhibition studies, and this is the first time that inhibitors of CT biosynthesis in human cancer cells were studied, allowing the direct observation of the effects on CT levels and its prohormones. As CPE and PAM are involved in the specific cleavage of C-terminal basic amino acids and the last step of peptide bioactivation, respectively, CPE was also targeted to moderate CT levels. The newly developed whole cell PAM inhibition assay allowed direct determination of the effects of PAM and CPE inhibition on CT production.
Peptide And Protein Hormones
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Author : Dr. Wolfgang König
language : en
Publisher:
Release Date : 1993
Peptide And Protein Hormones written by Dr. Wolfgang König and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.
Incorporates the developments in the field during the 25 years since the last major overview of peptide and protein hormones. Reviews the most important features of the hormones, including structure/activity relationships. Also describes their medical use, noting that western society encourages an overabundance of aggressive stress hormones, and that the role of doctors is to return them into balance with cooperative sex hormones. Of particular use to researchers, students, and practitioners is a special listing of the hormones according to a network of structural similarity and regulating cascades. Annotation copyright by Book News, Inc., Portland, OR
Hypothalamic Peptide Hormones And Pituitary Regulation
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Author : John Porter
language : en
Publisher: Springer
Release Date : 1977-08
Hypothalamic Peptide Hormones And Pituitary Regulation written by John Porter and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977-08 with Medical categories.
During the present and third workshop, the phrase hypothalamic releasing hormone and hypothalamic releasing factor were used synonomously. However, the demonstration of hypothalamic releasing hormones or factors in extrahypothalamic regions of the brain as well as in some non-neural tissues may denote a deficiency in the present nomenclature of this class of substances. This deficiency not withstanding, it was evident from the presentations of the participants that the progress in neuroendocrinology in the interval between the second and third workshops had been substantial if not extraordinary. Moreover, the accomplishments of the research endeavors of the experimental laboratories are now finding use in clinical settings. And, it is reassuring to see that what is learned in one species is so generally applicable to another, even man. Such findings increase confidence in our generalizations.
Cellular Peptide Hormone Synthesis And Secretory Pathways
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Author : Jens F. Rehfeld
language : en
Publisher: Springer
Release Date : 2010-10-22
Cellular Peptide Hormone Synthesis And Secretory Pathways written by Jens F. Rehfeld and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-22 with Science categories.
The concept of hormonal regulation using intercellular peptide messengers dates back to the discovery of secretin in 1902. The concept was simple: A peptide is released from specific hormone producing cells, endocrine cells, into circulation upon stimulation of the cells. The peptide hormone travels via blood to its target, the cells of which are equipped with specific receptors for high-affinity binding of the particular peptide hormone. Receptor binding subsequently elicits action of the target cells. This concept has been seriously challenged by modern biochemistry and cell biology. Thus, it is now well established that the gene of a specific peptide hormone may be expressed in different types of endocrine cells, in neurons, and in some instances also in adipocytes, myocytes, osteoblasts, and immune cells. Today, only a few hormones – including the old master hormone insulin – represent the original endocrine paradigm. Instead, the widespread cellular synthesis now raises the qu- tion of how the body maintains the regulation of its functions by peptide hormones when a hormone may originate from a variety of cells.
Cellular Peptide Hormone Synthesis And Secretory Pathways
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Author : Jens F. Rehfeld
language : en
Publisher: Springer
Release Date : 2010-03-11
Cellular Peptide Hormone Synthesis And Secretory Pathways written by Jens F. Rehfeld and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-11 with Science categories.
The concept of hormonal regulation using intercellular peptide messengers dates back to the discovery of secretin in 1902. The concept was simple: A peptide is released from specific hormone producing cells, endocrine cells, into circulation upon stimulation of the cells. The peptide hormone travels via blood to its target, the cells of which are equipped with specific receptors for high-affinity binding of the particular peptide hormone. Receptor binding subsequently elicits action of the target cells. This concept has been seriously challenged by modern biochemistry and cell biology. Thus, it is now well established that the gene of a specific peptide hormone may be expressed in different types of endocrine cells, in neurons, and in some instances also in adipocytes, myocytes, osteoblasts, and immune cells. Today, only a few hormones – including the old master hormone insulin – represent the original endocrine paradigm. Instead, the widespread cellular synthesis now raises the qu- tion of how the body maintains the regulation of its functions by peptide hormones when a hormone may originate from a variety of cells.
Peptide Therapeutics
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Author : Ved Srivastava
language : en
Publisher: Royal Society of Chemistry
Release Date : 2019-08-28
Peptide Therapeutics written by Ved Srivastava and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with Science categories.
Peptide therapy has become a key strategy in innovative drug development, however, one of the potential barriers for the development of novel peptide drugs in the clinic is their deficiencies in clearly defined chemistry, manufacturing and controls (CMC) strategy from clinical development to commercialization. CMC can often become a rate-limiting step due to lack of knowledge and lack of a formal policy or guidelines on CMC for peptide-based drugs. Regulators use a risk-based approach, reviewing applications on a case-by-case basis. Peptide Therapeutics: Strategy and Tactics for Chemistry, Manufacturing, and Controls covers efficient manufacturing of peptide drug substances, a review of the process for submitting applications to the regulatory authority for drug approval, a holistic approach for quality attributes and quality control from a regulatory perspective, emerging analytical tools for the characterisation of impurities, and the assessment of stability. This book is an essential reference work for students and researchers, in both academia and industry, with an interest in learning about CMC, and facilitating development and manufacture of peptide-based drugs.
Trends In Regulatory Peptides
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Author : Hubert Vaudry
language : en
Publisher: Frontiers Media SA
Release Date : 2018-07-24
Trends In Regulatory Peptides written by Hubert Vaudry and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-07-24 with categories.
Regulatory peptides represent the most diverse and versatile family of messenger molecules. They are produced by all living organisms from bacteria to mammals. They are involved in a wide variety of biological functions. Biologically active peptides and their receptors thus constitute an unlimited source of inspiration for the development of innovative drugs and cosmetics. The present eBook is a unique collection of research articles and reviews that provide a representative examplification of the latest progress in regulatory peptide research.
Peptide Hormone Receptors
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Author : Mohammed Y. Kalimi
language : en
Publisher:
Release Date : 1987
Peptide Hormone Receptors written by Mohammed Y. Kalimi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Medical categories.
Solid Phase Peptide Synthesis
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Author : Gregg B. Fields
language : en
Publisher: Academic Press
Release Date : 1997-10-21
Solid Phase Peptide Synthesis written by Gregg B. Fields and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-10-21 with Medical categories.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences. Key Features * Solid-phase peptide synthesis * Applications of peptides for structural and biological studies * Characterization of synthetic peptides
Clinical Neuroendocrinology
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Author : Michael Wilkinson
language : en
Publisher: Cambridge University Press
Release Date : 2019-01-03
Clinical Neuroendocrinology written by Michael Wilkinson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-03 with Medical categories.
A concise and innovative account of clinical neuroendocrine disorders and the key principles underlying their diagnosis and management.