Fluorescence Lifetime Spectroscopy And Imaging

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Fluorescence Lifetime Spectroscopy And Imaging
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Author : Laura Marcu
language : en
Publisher: CRC Press
Release Date : 2014-07-17
Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-17 with Science categories.
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia
Fluorescence Lifetime Imaging Ophthalmoscopy
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Author : Martin Zinkernagel
language : en
Publisher: Springer
Release Date : 2019-07-29
Fluorescence Lifetime Imaging Ophthalmoscopy written by Martin Zinkernagel and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-29 with Medical categories.
This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.
Fluorescence Lifetime Spectroscopy And Imaging
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Author : Laura Marcu
language : en
Publisher:
Release Date : 2014-01-01
Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-01 with Diagnostic imaging categories.
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Time Correlated Single Photon Counting
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Author : Desmond O'Connor
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Time Correlated Single Photon Counting written by Desmond O'Connor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.
New Trends In Fluorescence Spectroscopy
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Author : Bernard Valeur
language : en
Publisher: Springer Science & Business Media
Release Date : 2001
New Trends In Fluorescence Spectroscopy written by Bernard Valeur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.
This first volume in the new Springer Series on Fluorescencebrings together fundamental and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of state-of-the-art articles will serve as an invaluable tool for practitioners and novices and provide inspiration for new developments and applications.
Handbook Of Fluorescence Spectroscopy And Imaging
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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23
Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
High Resolution Imaging In Microscopy And Ophthalmology
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Author : Josef F. Bille
language : en
Publisher: Springer
Release Date : 2019-08-13
High Resolution Imaging In Microscopy And Ophthalmology written by Josef F. Bille and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Medical categories.
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Fluorescence Spectroscopy And Microscopy
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Author : Yves Engelborghs
language : en
Publisher:
Release Date : 2014
Fluorescence Spectroscopy And Microscopy written by Yves Engelborghs and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Fluorescence microscopy categories.
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Principles Of Fluorescence Spectroscopy
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-05
Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-05 with Science categories.
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.