Fluorescence Lifetime Spectroscopy And Imaging

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Fluorescence Lifetime Spectroscopy And Imaging

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Author : Laura Marcu
language : en
Publisher: CRC Press
Release Date : 2014-07-17

Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-17 with Technology & Engineering categories.

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.

Fluorescence Lifetime Spectroscopy And Imaging

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Author : Laura Marcu
language : en
Publisher:
Release Date : 2014-01-01

Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-01 with Diagnostic imaging categories.

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.

Handbook Of Fluorescence Spectroscopy And Imaging

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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23

Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Fluorescence Spectroscopy Imaging And Probes

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Author : Ruud Kraayenhof
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Fluorescence Spectroscopy Imaging And Probes written by Ruud Kraayenhof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.

Fluorescence Spectroscopy And Microscopy

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Author : Yves Engelborghs
language : en
Publisher: Humana
Release Date : 2016-08-23

Fluorescence Spectroscopy And Microscopy written by Yves Engelborghs and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Flim Microscopy In Biology And Medicine

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Author : Ammasi Periasamy
language : en
Publisher: CRC Press
Release Date : 2009-07-06

Flim Microscopy In Biology And Medicine written by Ammasi Periasamy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-06 with Science categories.

Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be

Handbook Of Biomedical Fluorescence

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Author : Mary-Ann Mycek
language : en
Publisher: CRC Press
Release Date : 2019-07-17

Handbook Of Biomedical Fluorescence written by Mary-Ann Mycek and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-17 with Medical categories.

Melding basic and clinical science, this reference provides a comprehensive overview of the roles that biophysics, photochemistry, and computational modeling play in the biomedical applications of fluorescence spectroscopy and imaging. Penned by pioneering researchers, the Handbook of Biomedical Fluorescence discusses fundamental aspects of fluorescence generation in organic molecules within tissue, theoretical and experimental views of how light propagation in tissue can be used to interpret fluorescence signals, endogenous and exogenous fluorescence agents in medical or basic research studies, and radiation transport, diffusion theory, and the Monte Carlo method.

Topics In Fluorescence Spectroscopy

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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-18

Topics In Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-18 with Science categories.

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Multiphoton Microscopy And Fluorescence Lifetime Imaging

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Author : Karsten König
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2018-01-22

Multiphoton Microscopy And Fluorescence Lifetime Imaging written by Karsten König and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-22 with Science categories.

This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.

Fundamentals Of Fluorescence Imaging

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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2019-04-23

Fundamentals Of Fluorescence Imaging written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-23 with Science categories.

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.