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Fluorescence Methods And Applications


Fluorescence Methods And Applications
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Chlorophyll A Fluorescence In Aquatic Sciences Methods And Applications


Chlorophyll A Fluorescence In Aquatic Sciences Methods And Applications
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Author : David J. Suggett
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-08-23

Chlorophyll A Fluorescence In Aquatic Sciences Methods And Applications written by David J. Suggett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-23 with Science categories.


Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.



Fluorescence Methods And Applications


Fluorescence Methods And Applications
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Author : Otto S. Wolfbeis
language : en
Publisher: Wiley-Blackwell
Release Date : 2008-07

Fluorescence Methods And Applications written by Otto S. Wolfbeis and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-07 with Science categories.


This volume features papers on new spectroscopic methods and techniques, the development and application of fluorescent probes, and new techniques and applications of fluorescence imaging. Specific areas include the following: fluorescence lifetime, fluorescence (in vivo) imaging, time-resolved fluorescence, luminescence anisotropy, fluorescent (NMIR) labels, luminescent lanthanides, fluorescent sensors and probes, fluorescence microscopy, FRET, fluorescent nanoparticles and dots, high-throughput screening, fluorescent bioassays, luminescence-based DNA technologies, FISH and immunohistochemistry, luminescence on metal surfaces, fluorescent proteins, upconversion, multiphoton fluorescence, confocal techniques, near-field and far-field techniques, single photon counting, fluorescence correlation spectroscopy (FCS), and flow cytometry. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit www.nyas.org/membership/main.asp for more information about becoming a member.



Molecular Fluorescence


Molecular Fluorescence
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Author : Bernard Valeur
language : en
Publisher: John Wiley & Sons
Release Date : 2013-03-25

Molecular Fluorescence written by Bernard Valeur and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-25 with Science categories.


Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.



New Trends In Fluorescence Spectroscopy


New Trends In Fluorescence Spectroscopy
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Author : Bernard Valeur
language : en
Publisher: Springer Science & Business Media
Release Date : 2001

New Trends In Fluorescence Spectroscopy written by Bernard Valeur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.


This first volume in the new Springer Series on Fluorescencebrings together fundamental and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of state-of-the-art articles will serve as an invaluable tool for practitioners and novices and provide inspiration for new developments and applications.



Fluorescence Applications In Biotechnology And Life Sciences


Fluorescence Applications In Biotechnology And Life Sciences
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Author : Ewa M. Goldys
language : en
Publisher: John Wiley & Sons
Release Date : 2009-08-24

Fluorescence Applications In Biotechnology And Life Sciences written by Ewa M. Goldys and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-08-24 with Science categories.


A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.



Fluorescence Lifetime Spectroscopy And Imaging


Fluorescence Lifetime Spectroscopy And Imaging
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Author : Laura Marcu
language : en
Publisher: CRC Press
Release Date : 2014-07-17

Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-17 with Science categories.


During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia



Fluorescence In Industry


Fluorescence In Industry
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Author : Bruno Pedras
language : en
Publisher: Springer
Release Date : 2019-06-14

Fluorescence In Industry written by Bruno Pedras and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-14 with Science categories.


This book gathers 12 outstanding contributions that reflect state-of-the-art industrial applications of fluorescence, ranging from the pharmaceutical and cosmetics industries to explosives detection, aeronautics, instrumentation development, lighting, photovoltaics, water treatment and much more. In the field of fluorescence, the translation of research into important applications has expanded significantly over the past few decades. The 18th volume in the Springer Series on Fluorescence fills an important gap by focusing on selected industrial applications of fluorescence, described in contributions by both industry-based researchers and academics engaged in collaborations with industrial partners.



Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy


Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy
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Author : T.G. Dewey
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy written by T.G. Dewey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.


Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.



Fluorescence Correlation Spectroscopy


Fluorescence Correlation Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Fluorescence Correlation Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


Fluorescence correlation spectroscopy (FCS) was developed in order to char acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea surements of spontaneous microscopic thermally driven molecular concentra tion fluctuations. Since its inception, and particularly in recent years, techni cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic concentration scale and the currently developing field of single molecule measurements. Both FCS and single mole cule approaches measure directly stochastic fluctuations in molecular pro perties, and so must be analyzed by statistical methods to yield conventional phenomenological parameters. As commonly practiced, FCS yields these phe nomenological parameters, e. g. , diffusion coefficients and chemical rate con stants, directly in terms of a fluorescence fluctuation autocorrelation func tion.



X Ray Fluorescence Spectroscopy For Laboratory Applications


X Ray Fluorescence Spectroscopy For Laboratory Applications
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Author : Michael Haschke
language : en
Publisher: John Wiley & Sons
Release Date : 2021-04-05

X Ray Fluorescence Spectroscopy For Laboratory Applications written by Michael Haschke and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-05 with Science categories.


Provides comprehensive coverage on using X-ray fluorescence for laboratory applications This book focuses on the practical aspects of X-ray fluorescence (XRF) spectroscopy and discusses the requirements for a successful sample analysis, such as sample preparation, measurement techniques and calibration, as well as the quality of the analysis results. X-Ray Fluorescence Spectroscopy for Laboratory Applications begins with a short overview of the physical fundamentals of the generation of X-rays and their interaction with the sample material, followed by a presentation of the different methods of sample preparation in dependence on the quality of the source material and the objective of the measurement. After a short description of the different available equipment types and their respective performance, the book provides in-depth information on the choice of the optimal measurement conditions and the processing of the measurement results. It covers instrument types for XRF; acquisition and evaluation of X-Ray spectra; analytical errors; analysis of homogeneous materials, powders, and liquids; special applications of XRF; process control and automation. An important resource for the analytical chemist, providing concrete guidelines and support for everyday analyses Focuses on daily laboratory work with commercially available devices Offers a unique compilation of knowledge and best practices from equipment manufacturers and users Covers the entire work process: sample preparation, the actual measurement, data processing, assessment of uncertainty, and accuracy of the obtained results X-Ray Fluorescence Spectroscopy for Laboratory Applications appeals to analytical chemists, analytical laboratories, materials scientists, environmental chemists, chemical engineers, biotechnologists, and pharma engineers.