Gel Permeation And Ion Exchange Chromatography Of Proteins And Peptides
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Gel Permeation And Ion Exchange Chromatography Of Proteins And Peptides
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Author : Kato
language : en
Publisher: CRC Press
Release Date : 2020-03-24
Gel Permeation And Ion Exchange Chromatography Of Proteins And Peptides written by Kato and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-24 with Science categories.
This book is a collection of critical reviews of the use of high-performance liquid chromatography in a very specialized area of research. It describes in detail modern methodology to separate nucleic acids, enzymes and a wide variety of biologically active proteins such as renin.
Ion Exchange Chromatography Of Proteins
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Author : Shuichi Yamamoto
language : en
Publisher: CRC Press
Release Date : 1988-04-14
Ion Exchange Chromatography Of Proteins written by Shuichi Yamamoto and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-04-14 with Science categories.
Showing chemists how to predict ion-exchange chromatography (IEC) separation behaviour and how to determime appropriate operating conditions, this reference illustrates procedures, apparatus and types of ion exchanges, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, this text explains the effects of each variable on separation behaviour; compares models and equations describing behaviour of proteins in IEC with experimental results; introduces design calculation procedures for scale up, adapting IEC for individual requirements; discusses parameters affecting separation behaviour, including adsorption equilibria, stationary phase diffusion and axial dispersion; provides examples of applications, such as high or medium performance IEC and large scale operations and reviews both theorectical and experimental literature. This text serves as an reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists and biotechnologists.
High Performance Liquid Chromatography Of Peptides And Proteins
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Author : Colin T. Mant
language : en
Publisher: CRC Press
Release Date : 2017-11-22
High Performance Liquid Chromatography Of Peptides And Proteins written by Colin T. Mant and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-22 with Medical categories.
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Peptides And Proteins
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Author : Shawn Doonan
language : en
Publisher: Royal Society of Chemistry
Release Date : 2002
Peptides And Proteins written by Shawn Doonan and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Education categories.
Encompassing all aspects of the structures of peptides and proteins, this book adopts a uniquely problem-oriented approach to the topic. Starting with a look at the structures and properties of the twenty amino acids that occur in proteins, and moving on to the synthesis of polypeptides and the isolation of proteins, Peptides and Proteins then addresses the methods of analysis of protein characteristics, including the modern methods of sequence analysis by mass spectrometry. Further chapters examine the three-dimensional nature of protein structure, and introduce the student to the use of computer applications (molecular graphics, databases, bioinformatics) in protein chemistry. Original research data is used in many of the problems, and throughout sufficient background biology is included, thus putting the subject into context for chemists. Aimed at first and second-year chemistry students, this title will also be of interest to students of biochemistry. Ideal for the needs of undergraduate chemistry students, Tutorial Chemistry Texts is a major new series consisting of short, single topic or modular texts concentrating on the fundamental areas of chemistry taught in undergraduate science courses. Each book provides a concise account of the basic principles underlying a given subject, embodying an independent-learning philosophy and including worked examples.
High Performance Liquid Chromatography Of Proteins And Peptides
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Author : Milton T. W. Hearn
language : en
Publisher:
Release Date : 1983-01-01
High Performance Liquid Chromatography Of Proteins And Peptides written by Milton T. W. Hearn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983-01-01 with High performance liquid chromatography categories.
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studie...
Methods Of Protein Separation
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Author : Nicholas Catsimpoolas
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Methods Of Protein Separation written by Nicholas Catsimpoolas and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
This open-end treatise on methods concerning pro tein separation had its beginning in an American Chemical Society symposium entitled "Con temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse quently of the present work-was to review the available modern techniques and underlying principles för achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there fore involved mostly mass transport processes. Additionally, the presentation of methods for gaining insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection and/or modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of pro teins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area. Nicholas Catsimpoolas Cambridge, Massachusetts vü CONTENTS Contents of Volume 1 . xvii Chapter 1 Scanning Gel Cbromatography Gary K. Ackers I.
Methods Of Protein Analysis
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Author : István Kerese
language : en
Publisher: Ellis Horwood
Release Date : 1984
Methods Of Protein Analysis written by István Kerese and has been published by Ellis Horwood this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Science categories.
Abstract: A reference text for food scientists, analysts, and biochemists presents a systematic, comprehensive survey of working procedures and methods for the analysis of proteins, peptides, and amino acids of varying origin and composition that can be used with equipment that generally is available in the average laboratory. Topics include: general laboratory methods for the preparation and characterization of proteinaceous materials; electrophoreticmethods (especially using polyacrylamide gels); thin-layer chromatographic methods; gel chromatography and gel filtration methods; and experimental procedures for nitrogendetermination, amino acid composition, electrophoresis, isoelectric focusing, ion-exchange chromatography, and gel filtration. Step-by-step descriptions are provided for sample preparation for analysis and problems requiring resolution are discussed.
Crc Handbook Of Chromatography
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Author : Ram N. Gupta
language : en
Publisher: CRC Press
Release Date : 2018-04-17
Crc Handbook Of Chromatography written by Ram N. Gupta and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-17 with Science categories.
These volumes provide a reference source of different gas chromatographic, liquid chromatographic, or thin-layer chromatographic techniques for the qualitative determination of various therapeutic agents, including antibiotics, vitamins and hormones, drugs of abuse in body fluids, dosage forms, or food stuffs. Over 5000 publications were reviewed to prepare tables of chromatographic data for 800 compounds, arranged alphabetically by generic drug name or by drug groups. A detailed summary of the extraction procedure described in each publication included in the table of a particular drug is also provided. This easy-to-read handbook is useful for selecting an appropriate chromatographic procedure for the determination of a given compound according to the available facilities.
Protein Purification Protocols
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Author : Paul Cutler
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Protein Purification Protocols written by Paul Cutler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Protein Purification
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Author : Jan-Christer Janson
language : en
Publisher: John Wiley & Sons
Release Date : 1998
Protein Purification written by Jan-Christer Janson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.