Gene Synthesis
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Gene Synthesis
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Author : Jean Peccoud
language : en
Publisher: Humana Press
Release Date : 2012-02-10
Gene Synthesis written by Jean Peccoud and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-10 with Medical categories.
The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.
Gene Synthesis Handbook
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Author : GenScript USA Inc
language : en
Publisher:
Release Date : 2013
Gene Synthesis Handbook written by GenScript USA Inc and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with DNA categories.
Gene Synthesis From Photolithographic Dna Microarrays
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Author : Changhan Kim
language : en
Publisher:
Release Date : 2007
Gene Synthesis From Photolithographic Dna Microarrays written by Changhan Kim and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Synthesis And Applications Of Dna And Rna
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Author : Saran Narang
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Synthesis And Applications Of Dna And Rna written by Saran Narang and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Synthesis and Applications of DNA and RNA discusses the significant contributions in the development of synthetic routes to DNA and RNA. This book contains nine chapters that describe the complexities in the chemistry and biology of DNA and RNA. After briefly dealing with the various stages of development in the chemical synthesis of polynucleotides, this book goes on presenting the DNA synthesis on solid supports and through the phosphoramidite method on silica supports. The discussions then shift to the chemical-enzymatic synthesis of expressed genes; the biochemical aspects of chemical syntheses of oligoribonucleotides; and the methods of rapid DNA and RNA sequence analysis. A chapter specifically tackles the protocols of DNA synthesis using double-stranded plasmid DNA as a template. The final chapter deals with the use of oligonucleotides for the identification and isolation of specific gene sequences. This chapter also covers the use oligonucleotides in the detection of human genetic diseases. Biologists, geneticists, and researchers interested in DNA and RNA synthesis will find this work invaluable.
Gene Synthesis By Assembly Of Short Oligonucleotides
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Author :
language : en
Publisher:
Release Date : 2005
Gene Synthesis By Assembly Of Short Oligonucleotides written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.
In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. This approach requires computational methods to accurately determine the assembly procedure, but relieves the current technological constraints of custom oligonucleotide synthesis. In order to assess the feasibility of such an approach, I examined T4 DNA Ligase activity on short oligonucleotides and found that ligation is dependent on the formation of a double-stranded DNA duplex of at least five base pairs flanking the site of ligation. However, ligations could be performed with overhangs smaller than five nucleotides and oligonucleotides as small as octamers, in the presence of a second, complementary oligonucleotide. As a proof of principle for DNA synthesis through the assembly of short oligonucleotides, I performed a hierarchical ligation procedure whereby octamers were combined to construct a target 128 bp segment of the human beta- ctin gene coding sequence. Thus, the construction of synthetic genes, without the need for custom oligonucleotide synthesis, is feasible. Algorithmic methods were then developed to extend this approach to DNA on the order of thousands of base pairs.
Towards An Inexpensive High Thoughroughput Method Of Automated Gene Synthesis
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Author : Michael John Bassetti
language : en
Publisher:
Release Date : 2007
Towards An Inexpensive High Thoughroughput Method Of Automated Gene Synthesis written by Michael John Bassetti and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Novel Algorithms For In Vitro Gene Synthesis
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Author : Chris Thachuk
language : en
Publisher:
Release Date : 2007
Novel Algorithms For In Vitro Gene Synthesis written by Chris Thachuk and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Genetic algorithms categories.
Methods for reliable synthesis of long genes offer great promise for novel protein synthesis via expression of synthetic genes. Current technologies use computational methods for design of short oligos, which can then be reliably synthesized and assembled into the desired target gene. A precursor to this process is optimization of the gene sequence for improved protein expression. In this thesis, we provide the first results on the computational complexity of oligo design for gene synthesis. For collision-oblivious oligo design -- when mishybridizations between oligos are ignored -- we give an efficient dynamic programming algorithm. We show that an abstraction of the collision-aware oligo design problem is NP-hard. We extend our collision-oblivious algorithm to achieve collision-aware oligo design, when the gene can be partitioned into independently-assembled segments. We propose additional algorithms for gene optimization and demonstrate their utility for collision-aware design. All methods are evaluated on a large biological gene set.
Dna As A Programmable Material
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Author : Samuel James Hwang
language : en
Publisher:
Release Date : 2008
Dna As A Programmable Material written by Samuel James Hwang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering.
Synthetic Genomics
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Author : Fouad Sabry
language : en
Publisher: One Billion Knowledgeable
Release Date : 2022-10-05
Synthetic Genomics written by Fouad Sabry and has been published by One Billion Knowledgeable this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-05 with Medical categories.
What Is Synthetic Genomics To manufacture new DNA or complete lifeforms, synthetic genomics, a relatively young subfield of synthetic biology, employs techniques such as genetic alteration on already-existent life forms or artificial gene synthesis. These techniques may be used to create new DNA. How You Will Benefit (I) Insights, and validations about the following topics: Chapter 1: Synthetic genomics Chapter 2: Base pair Chapter 3: Bacterial artificial chromosome Chapter 4: Molecular genetics Chapter 5: Yeast artificial chromosome Chapter 6: DNA synthesis Chapter 7: Site-directed mutagenesis Chapter 8: Xenobiology Chapter 9: Index of molecular biology articles Chapter 10: DNA construct Chapter 11: Genomic library Chapter 12: Fosmid Chapter 13: Artificial gene synthesis Chapter 14: Functional cloning Chapter 15: Mycoplasma laboratorium Chapter 16: Nucleic acid analogue Chapter 17: Molecular cloning Chapter 18: Minimal genome Chapter 19: Clyde A. Hutchison III Chapter 20: Synthetic genomes Chapter 21: No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing (II) Answering the public top questions about synthetic genomics. (III) Real world examples for the usage of synthetic genomics in many fields. (IV) 17 appendices to explain, briefly, 266 emerging technologies in each industry to have 360-degree full understanding of synthetic genomics' technologies. Who This Book Is For Professionals, undergraduate and graduate students, enthusiasts, hobbyists, and those who want to go beyond basic knowledge or information for any kind of synthetic genomics.
Chemical And Enzymatic Synthesis Of Gene Fragments
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Author : Hans Günter Gassen
language : en
Publisher:
Release Date : 1982
Chemical And Enzymatic Synthesis Of Gene Fragments written by Hans Günter Gassen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Cell cycle categories.
Preface: In the past the chemical and enzymatic synthesis of oligonucleotides of defined sequence had to be left to a few experts. Now, however, with the triester approach, the phosphite method and the solid-support techniques gene fragment synthesis has turned into an easy procedure even for a non-chemist. Due to the elegant chemistry involved, all methods work without sophisticated equipment and are prone to mechanisation and eventual automation. It is hoped that combined chemical-encymatic gene synthesis may become a standard technique in a molecular biology laboratory, such as DNA sequencing or in-vitro recombination of nucleic acides. We omitted chemical RNA synthesis, since this field is developing so rapidly at the moment that one has to refer to the original publications. However, we included enzymatic synthesis of RNA fragments, procedures which already have obtained a high degree of standardisation. Most of the contributions are revised versions of the protocols supplied for the EMBO sponsored course on "Automated Chemical and Enzymic Gene Synthesis", held in Darmstadt, March 21 to April 3, 1982. The protocols were improved on the basis of the experience of 30 student scientists with chemical, biological or medical backgrounds. Previously omitted procedures, such as the wandering spot method for oligonucleotide analysis, were included. In editing the manuscript we encountered problems with the nomenclature of nucleic acid components. In unambiquous cases we favoured a simple description, hoping for example, that oligodeoxynucleotide is always understood to mean oligo-2'-deoxyribonucleotide. This book aims to provide those interested in DNA/RNA research with state-of-the-art methods in the synthesis, purification, and analysis of DNA and RNA fragments. The editors wish to thank the authors for their efforts in preparing manuscripts from the the revised laboratory protocols. We gratefully acknowledge the skill and the patience of Mrs. E. Ronnfeldt in typing the manuscripts. We express our thanks to Verlag Chemie for the friendly and very efficient cooperation.--H.G. Gassen A. Lang--Darmstadt, in July 1982.