Gene Transfer And Expression Protocols

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Plant Gene Transfer And Expression Protocols

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Author : Heddwyn Jones
language : en
Publisher: U of Nebraska Press
Release Date : 1995-09-27

Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by U of Nebraska Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-27 with Science categories.

In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only a complete collection of "mainstream" methods, but also methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. There are also methods for introducing and analyzing plant genes in Xenopus and heterologous yeast hosts. Plant Gene Transfer and Expression Protocols is an indispensable tool for scientists working in both fundamental (biochemical, physiological, and genetic) and applied aspects of plant biotechnology, plant breeding, agriculture, and horticulture. It provides concise practical help that produces the desired results even for beginners and opens the doors to new research possibilities.

Gene Transfer And Expression Protocols

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Author : Edward J. Murray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Gene Transfer And Expression Protocols written by Edward J. Murray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .

Plant Gene Transfer And Expression Protocols

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Author : Heddwyn Jones
language : en
Publisher: Humana
Release Date : 2013-08-21

Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-21 with Science categories.

The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.

Protocols For Gene Transfer In Neuroscience

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Author : P. R. Lowenstein
language : en
Publisher:
Release Date : 1996-07-25

Protocols For Gene Transfer In Neuroscience written by P. R. Lowenstein and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-07-25 with Medical categories.

Protocols for Gene Transfer in Neuroscience Towards Gene Therapy of Neurological Disorders Edited by P. R. Lowenstein University of Manchester, UK and L. W. Enquist Princeton University, USA The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as needed. Written by a team of international expert neuroscientists, geneticists, molecular biologists and biochemists, Protocols for Gene Transfer in Neuroscience describes the latest techniques from the top gene-delivery labs, ranging from physical transfection techniques, through the use of a wide range of viral vectors, to retrovirally transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.

Molecular Biology And Biotechnology

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Author : John M. Walker
language : en
Publisher: Royal Society of Chemistry
Release Date : 2000

Molecular Biology And Biotechnology written by John M. Walker and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Education categories.

This volume provides a revised and updated introduction to the techniques of molecular biology and its industrial applications. It should be of particular benefit to undergraduates and researchers in other biological areas.

Gene Transfer To Plants

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Author : Ingo Potrykus
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Gene Transfer To Plants written by Ingo Potrykus and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.

Plant Gene Transfer And Expression Protocols

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Author : Heddwyn Jones
language : en
Publisher: Humana Press
Release Date : 1995-09-27

Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-27 with Science categories.

The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.

Molecular Genetics Gene Transfer And Therapy

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Author :
language : en
Publisher: Elsevier
Release Date : 1997-10-01

Molecular Genetics Gene Transfer And Therapy written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-10-01 with Medical categories.

Animal science is being transformed by advances in molecular genetics. This book covers this exciting transformation. Both the diagnosis and treatment of disease are discussed in seven chapters by a team of leading international authorities. From oncogenesis and hemophilia to retroviral virulence and genome mapping, this volume yields an understanding of fundamental mechanisms of action and insightful means of diagnosis and treatment. Practicing veterinarians and researchers in animal science will find this book useful.

Handbook Of Molecular And Cellular Methods In Biology And Medicine

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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2003-11-24

Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-24 with Medical categories.

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

Advanced Textbook On Gene Transfer Gene Therapy And Genetic Pharmacology Principles Delivery And Pharmacological And Biomedical Applications Of Nucleotide Based Therapies Second Edition

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Author : Daniel Scherman
language : en
Publisher: World Scientific
Release Date : 2019-07-16

Advanced Textbook On Gene Transfer Gene Therapy And Genetic Pharmacology Principles Delivery And Pharmacological And Biomedical Applications Of Nucleotide Based Therapies Second Edition written by Daniel Scherman and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-16 with Science categories.

This unique advanced textbook provides a clear and comprehensive overview of gene delivery, gene therapy and genetic pharmacology, with descriptions of the main gene transfer vectors and a set of selected therapeutic applications, along with safety considerations. The second edition features new groundbreaking material on genome editing using the recently discovered CRISPR/Cas9 system and on cancer immunotherapy by CAR-T cells. It also presents the historical milestone of gene therapy application in the field of severe combined immunodeficiency, and other fields of gene therapy and molecular medicine.The use of gene transfer is exponentially growing in the scientific and medical communities for day-to-day cell biology experiments and swift development of gene therapy, which is already revolutionizing medicine. In this advanced textbook, more than 30 leading scientists come together to explore these topics.This educational introduction provides the background material needed to further explore the subject as well as relevant research literature. It is an invaluable resource to Master, PhD or MD students, post-doctoral scientists or medical doctors, as well as any scientist wishing to deliver a gene or synthetic nucleotide or develop a gene therapy strategy. The second edition's simple and synthetic content will be of value to any reader interested in the biological and medical revolution derived from the elucidation of the human genome.