Plant Gene Transfer And Expression Protocols
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Plant Gene Transfer And Expression Protocols
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Author : Heddwyn Jones
language : en
Publisher: U of Nebraska Press
Release Date : 1995-09-27
Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by U of Nebraska Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-27 with Science categories.
In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only a complete collection of "mainstream" methods, but also methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. There are also methods for introducing and analyzing plant genes in Xenopus and heterologous yeast hosts. Plant Gene Transfer and Expression Protocols is an indispensable tool for scientists working in both fundamental (biochemical, physiological, and genetic) and applied aspects of plant biotechnology, plant breeding, agriculture, and horticulture. It provides concise practical help that produces the desired results even for beginners and opens the doors to new research possibilities.
Plant Gene Transfer And Expression Protocols
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Author : Heddwyn Jones
language : en
Publisher: Humana
Release Date : 2013-08-21
Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-21 with Science categories.
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Gene Transfer To Plants
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Author : Ingo Potrykus
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Gene Transfer To Plants written by Ingo Potrykus and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
Molecular Biology And Biotechnology
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Author : John M Walker
language : en
Publisher: Royal Society of Chemistry
Release Date : 2007-10-31
Molecular Biology And Biotechnology written by John M Walker and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-31 with Science categories.
The 3rd Edition of this immensely popular textbook brings readers up to date with the latest advances in this rapidly developing field. It has been thoroughly revised and expanded, combining entirely new chapters on current hot topics with existing chapters that have been rewritten and extended. Molecular Biology and Biotechnology 3rd Edition provides real breadth of coverage, taking account of both molecular biology and industrial applications. It also gives in depth coverage of key areas, resulting in a uniquely comprehensive publication. Highlights of this 3rd Edition include increased coverage of the cloning of yeast and animal cells and plant genetic engineering, and new coverage of polymerase chain reactions, DNA fingerprinting, transgenesis and vaccine development, reflecting the rapid growth of interest in these areas. The book presents the information in an easily assimilated form and makes an ideal undergraduate text. It will be of particular interest to students of biology and chemistry, as well as to scientific workers from outside the field who need a rapid introduction to the subject. Molecular Biology and Biotechnology primarily has a teaching function and as such should prove invaluable.
Plant Gene Transfer And Expression Protocols
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Author : Heddwyn Jones
language : en
Publisher: Humana Press
Release Date : 1995-09-27
Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-27 with Science categories.
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Transgenic Plants
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Author : Leandro Peña
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Transgenic Plants written by Leandro Peña and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.
Polyamine Protocols
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Author : David M. L. Morgan
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Polyamine Protocols written by David M. L. Morgan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.
Basic Cell Culture Protocols
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Author : Jeffrey W. Pollard
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Basic Cell Culture Protocols written by Jeffrey W. Pollard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
Protein Targeting Protocols
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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.
Immunochemical Protocols
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Author : John Pound
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Immunochemical Protocols written by John Pound and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.