Protein Targeting Protocols
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Protein Targeting Protocols
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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.
Protein Targeting Protocols
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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-01-14
Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-14 with Medical categories.
Master experimentalists describe, largely from the perspective of intracellular signaling, their best methods for detecting and analyzing the proteins and protein motifs involved in targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. This book will serve well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.
Flavoprotein Protocols
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Author : Steven K. Chapman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flavoprotein Protocols written by Steven K. Chapman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Plant Virology Protocols
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Author : Gary D. Foster
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Plant Virology Protocols written by Gary D. Foster and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
The Protein Protocols Handbook
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2002
The Protein Protocols Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Immunoinformatics
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Author : Darren R. Flower
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-07
Immunoinformatics written by Darren R. Flower and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-07 with Science categories.
This volume both engages the reader and provides a sound foundation for the use of immunoinformatics techniques in immunology and vaccinology. It addresses databases, HLA supertypes, MCH binding, and other properties of immune systems. The book contains chapters written by leaders in the field and provides a firm background for anyone working in immunoinformatics in one easy-to-use, insightful volume.
Receptor Binding Techniques
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Author : Mary Keen
language : en
Publisher: Springer Science & Business Media
Release Date : 1999
Receptor Binding Techniques written by Mary Keen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
This cutting-edge collection of step-by-step experimental protocols demonstrates
Mass Spectrometry Of Proteins And Peptides
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Author : John R. Chapman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Mass Spectrometry Of Proteins And Peptides written by John R. Chapman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.
Gus Protocols
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Author : Sean R. Gallagher
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Gus Protocols written by Sean R. Gallagher and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes. - First book on the GUS reporter system - Up-to-date reference lists following each of the fourteen chapters - Comb-bound for convenient bench-top use - Written by leading authorities including R.A. Jefferson, inventor of the GUS assay - Illustrated with color - GUS detection by fluorometric, spectrophotometric, and histochemical methods - Fast, automated assays
Molecular Methods In Developmental Biology
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Author : Matt Guille
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Molecular Methods In Developmental Biology written by Matt Guille and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.