Genetic Transformation And Expression


Genetic Transformation And Expression
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Genetic Transformation And Expression


Genetic Transformation And Expression
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Author : L. O. Butler
language : en
Publisher:
Release Date : 1990

Genetic Transformation And Expression written by L. O. Butler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Gene expression categories.




Gene Transfer And Expression In Mammalian Cells


Gene Transfer And Expression In Mammalian Cells
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Author : S.C. Makrides
language : en
Publisher: Gulf Professional Publishing
Release Date : 2003-10-24

Gene Transfer And Expression In Mammalian Cells written by S.C. Makrides and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-24 with Science categories.


This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.



Genetic Transformation


Genetic Transformation
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Author : María Alvarez
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-09-06

Genetic Transformation written by María Alvarez and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-06 with Medical categories.


Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.



Gene Transfer And Expression Protocols


Gene Transfer And Expression Protocols
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Author : Edward J. Murray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Gene Transfer And Expression Protocols written by Edward J. Murray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.


Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .



Plant Genetic Transformation Technology


Plant Genetic Transformation Technology
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Author : Wagdy Sawahel
language : en
Publisher: Daya Books
Release Date : 1997

Plant Genetic Transformation Technology written by Wagdy Sawahel and has been published by Daya Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Plant genetic transformation categories.


The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology. Contents Chapter 1: Genetic Transformation; History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability; Chapter 2: Gene Delivery systems; Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication; Chapter 3: Strategies for Improving Transformation Efficiency; Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence; Chapter 4: Organelle Transformation; Chapter 5: Shotgun Transformation; Plasmid rescue, Gene rescue, Promoter & enhancer rescue.



Gene Transfer To Animal Cells


Gene Transfer To Animal Cells
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Author : Richard Twyman
language : en
Publisher: Taylor & Francis
Release Date : 2004-12-01

Gene Transfer To Animal Cells written by Richard Twyman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-12-01 with Science categories.


Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.



Plant Transformation Technologies


Plant Transformation Technologies
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Author : Charles Neal Stewart
language : en
Publisher: John Wiley & Sons
Release Date : 2011-01-31

Plant Transformation Technologies written by Charles Neal Stewart and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-01-31 with Science categories.


Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.



Genetic Transformation Of Plants


Genetic Transformation Of Plants
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Author : John Flex Jackson
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09

Genetic Transformation Of Plants written by John Flex Jackson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.


Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.



Gene Transfer And Expression


Gene Transfer And Expression
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Author : Michael Kriegler
language : en
Publisher: Springer
Release Date : 1990-06-18

Gene Transfer And Expression written by Michael Kriegler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-06-18 with Science categories.


A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.



Genetic Transformation In Crops


Genetic Transformation In Crops
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Author : Kin-Ying To
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-10-07

Genetic Transformation In Crops written by Kin-Ying To and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-07 with Medical categories.


Due to rapid population growth, climate change, and decreasing natural resources, growing sufficient crops with high productivity, resistance to abiotic and biotic stresses, and other attractive traits is a major challenge. Conventional breeding methods require time-consuming genetic crosses between different parents for multiple generations. By contrast, plant transformation is defined as the insertion of DNA from any organism into the genome of a plant species, and it is considered to be a powerful tool in plant breeding. This book aims to provide professional state-of-the-art information for basic and applied scientists and plant breeders, focusing on key crop plants. Papers related to the principle and application of Agrobacterium-mediated transformation, step-by-step protocols of DNA delivery to the important crop Brassica oleracea and higher-plant chloroplasts, current progress and prospects of virus-induced gene silencing (VIGS) in higher plants, improvement of grapevine through biotechnology, and public concern of biosafety issues regarding genetically modified organisms (GMOs) are all included in this book. It should be useful for students, breeders, and researchers in the field of transgenic crops around the world.