Gene Transfer And Expression
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Gene Transfer And Expression In Mammalian Cells
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Author : S.C. Makrides
language : en
Publisher: Gulf Professional Publishing
Release Date : 2003-10-24
Gene Transfer And Expression In Mammalian Cells written by S.C. Makrides and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-24 with Science categories.
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.
Gene Transfer And Expression Protocols
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Author : Edward J. Murray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Gene Transfer And Expression Protocols written by Edward J. Murray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
Gene Transfer And Expression
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Author : Michael Kriegler
language : en
Publisher: Springer
Release Date : 1990-06-18
Gene Transfer And Expression written by Michael Kriegler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-06-18 with Science categories.
A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
Plant Gene Transfer And Expression Protocols
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Author : Heddwyn Jones
language : en
Publisher: Humana
Release Date : 2013-08-21
Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-21 with Science categories.
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Transfer And Expression Of Eukaryotic Genes
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Author : H.S. Ginsberg
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Transfer And Expression Of Eukaryotic Genes written by H.S. Ginsberg and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Transfer and Expression of Eukaryotic Genes documents the progress in our understanding of the transfer and expression of eukaryotic genes. This book covers topics organized around three themes: gene expression and its regulation; in vivo gene transfer and development; and viral gene and oncogene systems. This text is divided into three sections encompassing 25 chapters and begins with an overview of the molecular basis of gene expression, with emphasis on transcription complexes that account for transcription control in eukaryotic genes. It then turns to experiments that assess the in vitro stimulatory effect of the SV40 72-bp repeat on specific transcription from heterologous promoter elements using a HeLa whole cell extract. The reader is methodically introduced to the regulation signals and factors of histone gene transcription; transcriptional control of beta-globin and liver-specific genes in mouse cells; and gene transfer in Drosophila and the sea urchin Strongylocentrotus purpuratus. This book also considers the splicing of messenger RNA precursors and the regulation of thymidine kinase enzyme expression, and then concludes with a chapter that describes the activation of the myc oncogene by chromosomal translocation. This book will be of interest to students and researchers in fields ranging from molecular genetics to microbiology, biochemistry, pathology, and immunology.
Gene Transfer
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Author : Theodore Friedmann
language : en
Publisher: CSHL Press
Release Date : 2007
Gene Transfer written by Theodore Friedmann and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Gene Transfer Techniques categories.
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Plant Gene Transfer And Expression Protocols
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Author : Heddwyn Jones
language : en
Publisher: Humana Press
Release Date : 1995-09-27
Plant Gene Transfer And Expression Protocols written by Heddwyn Jones and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-09-27 with Science categories.
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Gene Transfer To Animal Cells
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Author : Richard Twyman
language : en
Publisher: Taylor & Francis
Release Date : 2004-12-01
Gene Transfer To Animal Cells written by Richard Twyman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-12-01 with Science categories.
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Gene Therapeutics
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Author : Jon A. Wolff
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Gene Therapeutics written by Jon A. Wolff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
During the first half century of genetics, coinciding with the first half of this cen tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex tremely efficient in transferring their contents to the chromosomes of cultured cells.
Advanced Textbook On Gene Transfer Gene Therapy And Genetic Pharmacology Principles Delivery And Pharmacological And Biomedical Applications Of Nucleotide Based Therapies
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Author : Daniel Scherman
language : en
Publisher: World Scientific
Release Date : 2013-12-10
Advanced Textbook On Gene Transfer Gene Therapy And Genetic Pharmacology Principles Delivery And Pharmacological And Biomedical Applications Of Nucleotide Based Therapies written by Daniel Scherman and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-10 with Science categories.
This unique advanced textbook provides a clear and comprehensive description of the field of gene delivery, gene therapy and genetic pharmacology, with descriptions of the main gene transfer vectors and a set of selected therapeutic applications, along with safety considerations.The use of gene transfer is exponentially growing in the scientific and medical communities for day-to-day cell biology experiments and swift development of revolutionary gene therapy strategies. In this advanced textbook, more than 25 leading scientists, world-renowned in their respective fields, come together to provide a clear and comprehensive description of gene delivery, gene therapy and genetic pharmacology.This educational introduction to the main gene transfer vectors and selected therapeutic applications provides the background material needed to further explore the subject as well as relevant research literature. It will thus be invaluable to Master, PhD or MD students, post-doctoral scientists or medical doctors, as well as any scientist wishing to deliver a gene or synthetic nucleotide, or develop a gene therapy strategy. Furthermore, the textbook's simple and synthetic content will be of value to any reader interested in the biological and medical revolution derived from the elucidation of the human genome.