Genomic And Proteomic Techniques
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Genomic And Proteomic Techniques
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Author : Ranil S. Dassanayake
language : en
Publisher: Alpha Science International, Limited
Release Date : 2011
Genomic And Proteomic Techniques written by Ranil S. Dassanayake and has been published by Alpha Science International, Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Science categories.
Describes the high throughput sophisticated computational tools and experimental techniques used in post genomic analyses to harness the genomic information to make novel biological discoveries. Beginning with a chapter on bioinformatics that introduces readers to Word Wide Web based servers and software programs that are widely used for mining genomic information it moves on to a miniaturized high throughput technique, DNA microarrays, tailored for studying genome-scale genotyping and gene-expression profiling. An overview of another high throughput proteomic technique; two-dimensional polyacrylamide gel electrophoresis used in proteome separation and mass spectrometry and protein sequencing related techniques used for characterization and identification of proteins is followed by a description of yet another miniaturized high throughput proteomic technique, Protein Microarrays, developed to overcome the limitations associated with DNA microarray and to provide direct information about protein quantity, interactions, functions etc. Knowledge-based theoretical three-dimensional protein structure modeling approach; homology modeling as a realistic method to minimize the ever widening gap between known protein coding sequences and known protein structures and fully automated protein modeling software and servers implemented in protein structure modeling in the post genomic era are also discussed.
Concepts And Techniques In Genomics And Proteomics
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Author : N Saraswathy
language : en
Publisher: Elsevier
Release Date : 2011-07-01
Concepts And Techniques In Genomics And Proteomics written by N Saraswathy and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-01 with Science categories.
Concepts and techniques in genomics and proteomics covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained with its underlying concepts, and simple line diagrams and flow charts are included to aid understanding and memory. A summary of key points precedes each chapter within the book, followed by detailed description in the subsections. Each subsection concludes with suggested relevant original references. Provides definitions for key concepts Case studies are included to illustrate ideas Important points to remember are noted
Genomics And Proteomics
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Author : Devarajan Thangadurai
language : en
Publisher: CRC Press
Release Date : 2015-06-09
Genomics And Proteomics written by Devarajan Thangadurai and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-09 with Medical categories.
The book provides scope and knowledge on advanced techniques and its applications into the modern fields of biotechnology-genomics and proteomics. In this book, different genomics and proteomics technologies and principles are examined. The fundamental knowledge presented in this book opens up an entirely new way of approaching DNA chip technology,
Principles Of Genomics And Proteomics
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Author : Rakeeb Ahmad Mir
language : en
Publisher: Elsevier
Release Date : 2023-01-25
Principles Of Genomics And Proteomics written by Rakeeb Ahmad Mir and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-25 with Science categories.
Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments. Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms Includes detailed and standardized coverage of basic research methods in areas covered Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields
Proteome Analysis
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Author : David W Speicher
language : en
Publisher: Elsevier
Release Date : 2004-05-18
Proteome Analysis written by David W Speicher and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-18 with Science categories.
This book explores the current status of proteomics, an exciting new discipline, which is less than 10 years old. This new field has rapidly grown into a major commercial and research enterprise with great prospects for dramatically advancing our knowledge of basic biological and disease processes. The contributors to this book are an international panel of proteomics experts, who review and discuss the current status of specific technologies and approaches. Proteomics represents an exciting new way to pursue biological and biomedical science at an unprecedented pace. Proteomics takes a broad, comprehensive, systematic approach to understanding biology that is generally unbiased and not dependent upon existing knowledge. The major components of proteomics from basic discovery using a range of alternative analytical methods to discovery validation and use for clinical applications are discussed. State-of-the-art protein profiling methods include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also discussed. Additional chapters address the application of current proteome techniques to clinical problems and the availability of protein expression library resources for proteome studies. · Authored by international experts in the field · Covers a wide range of topics including 2-D gels, global proteomics using accurate mass tags, global proteomics using electrophoretic prefractionation, microfluidics, and nanotechnology · Includes state-of-the-art protein profiling methods, and emerging technologies
Fundamentals Of Data Mining In Genomics And Proteomics
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Author : Werner Dubitzky
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-04-13
Fundamentals Of Data Mining In Genomics And Proteomics written by Werner Dubitzky and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-04-13 with Science categories.
This book presents state-of-the-art analytical methods from statistics and data mining for the analysis of high-throughput data from genomics and proteomics. It adopts an approach focusing on concepts and applications and presents key analytical techniques for the analysis of genomics and proteomics data by detailing their underlying principles, merits and limitations.
Genomics Proteomics And Vaccines
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Author : Guido Grandi
language : en
Publisher: John Wiley & Sons
Release Date : 2004-08-13
Genomics Proteomics And Vaccines written by Guido Grandi and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08-13 with Science categories.
While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens. This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions
Principles Of Proteomics
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Author : Richard Twyman
language : en
Publisher: Garland Science
Release Date : 2004-05-01
Principles Of Proteomics written by Richard Twyman and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-01 with Science categories.
Principles of Proteomics is designed specifically to explain the different stages of proteomic analysis, their complexities and their jargon to students and researchers in a non-technical overview of the field. The author describes the broad range of problems which proteomics can address, including structural proteomics, interaction proteomics, protein modification analysis and functional proteomics. Methodologies are described in user-friendly language, from the more traditional two-dimensional gel electrophoresis to the new developments in protein chip technologies. These are well presented in the context of overall strategies which can be adopted to address the different aspects of large-scale protein analysis.
Introduction To Proteomics
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Author : Daniel Liebler
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-12-04
Introduction To Proteomics written by Daniel Liebler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-12-04 with Medical categories.
Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.
Proteomics In Functional Genomics
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Author : P. Jolles
language : en
Publisher: Birkhäuser
Release Date : 2013-03-11
Proteomics In Functional Genomics written by P. Jolles and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-11 with Science categories.
A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...