Glycoprotein And Proteoglycan Techniques
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Glycoprotein And Proteoglycan Techniques
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Author : J.G. Beeley
language : en
Publisher: Elsevier
Release Date : 2000-04-01
Glycoprotein And Proteoglycan Techniques written by J.G. Beeley and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-04-01 with Medical categories.
The past two decades have seen an expansion of interest in glycoproteins. From being a borderline area between carbohydrate and protein chemistry, it has become relevant to a wide range of biological phenomena. The aim of the book is to describe techniques which can be used to answer some of the basic questions about glycosylated proteins. Methods are discussed for isolation; compositional analysis; structure carbohydrate units; protein-carbohydrate linkages, keeping in mind the diverse nature of problems which readers may have to tackle.
Glycoprotein And Proteoglycan Techniques
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Author :
language : en
Publisher:
Release Date : 1989
Glycoprotein And Proteoglycan Techniques written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with categories.
Proteoglycan Protocols
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Author : Renato V. Iozzo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Proteoglycan Protocols written by Renato V. Iozzo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.
Proteoglycans
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Author : Françoise Rédini
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Proteoglycans written by Françoise Rédini and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.
The Biochemistry Of Glycoproteins And Proteoglycans
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Author : William Lennarz
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Biochemistry Of Glycoproteins And Proteoglycans written by William Lennarz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Although glycoproteins and proteoglycans have been a subject of re search for many years, it is only during the last five or so years that they have aroused the interest of a very broad cross section of investigators in the biological sciences. The reason for this expanded interest in these molecules is simple: not only are glycoproteins and proteoglycans ubiq uitous, but many are molecules with well-defined and important biological functions. The list of molecules that fall into this category grows daily; interferon, immunoglobulins, certain hormones, many cell surface recep tors, and viral coat proteins are but a few examples. Thus, investigators with interests as diverse as viral replication. cell-cell interactions. poly isoprenoid synthesis, secretory processes, hormone responses, embryonic development, and immunology have become concerned with glycopro teins and proteoglycans. The objective of this book is to summarize the current state of knowledge on the biochemistry of these molecules. Coverage is by no means encyclopedic; rather the thrust is to emphasize the recent ad vances. The first chapter deals primarily with structural work on the oligosaccharide chains of glycoproteins, but it will be apparent in it and in the succeeding two chapters on biosynthesis that not only do structural studies aid biosynthetic investigations, but that studies on biosynthesis often playa major role in elucidation of structure.
Glycan Based Cellular Communication
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Author : Peng George Wang
language : en
Publisher:
Release Date : 2020
Glycan Based Cellular Communication written by Peng George Wang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Carbohydrates categories.
Guide To Techniques In Glycobiology
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Author : William J. Lennarz
language : en
Publisher: Academic Press
Release Date : 1994-01-03
Guide To Techniques In Glycobiology written by William J. Lennarz and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-01-03 with Nature categories.
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates
Proteoglycans
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Author : John F. Kennedy
language : en
Publisher: Elsevier Science & Technology
Release Date : 1979
Proteoglycans written by John F. Kennedy and has been published by Elsevier Science & Technology this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with Medical categories.
Glycoanalysis Protocols
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Author : Elizabeth F. Hounsell
language : en
Publisher: Humana Press
Release Date : 1998-02-27
Glycoanalysis Protocols written by Elizabeth F. Hounsell and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-02-27 with Science categories.
Glycomics
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Author : Nicolle H. Packer
language : en
Publisher: Humana Press
Release Date : 2009-07-16
Glycomics written by Nicolle H. Packer and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-16 with Science categories.
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.