Gradient Elution In Column Liquid Chromatography
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Gradient Elution In Column Liquid Chromatography
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Author : P. Jandera
language : en
Publisher: Elsevier
Release Date : 1985-05-01
Gradient Elution In Column Liquid Chromatography written by P. Jandera and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-05-01 with Science categories.
Gradient Elution in Column Liquid Chromatography
Gradient Elution In Column Liquid Chromatography
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Author : P. Jandera
language : en
Publisher:
Release Date : 1985
Gradient Elution In Column Liquid Chromatography written by P. Jandera and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with categories.
High Performance Gradient Elution
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Author : Lloyd R. Snyder
language : en
Publisher: John Wiley & Sons
Release Date : 2007-01-09
High Performance Gradient Elution written by Lloyd R. Snyder and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-09 with Science categories.
Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.
Liquid Chromatography
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Author : J.W. Dolan
language : en
Publisher: Elsevier Inc. Chapters
Release Date : 2013-01-08
Liquid Chromatography written by J.W. Dolan and has been published by Elsevier Inc. Chapters this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-08 with Science categories.
Gradient elution is the method of choice for the separation of samples with a wide retention range. This chapter reviews the basic theory and practical consideration for method development in gradient elution. Optimization requires selection of the range of solvent composition, solvent type, the gradient time, and gradient shape. The linear solvent strength model of gradient elution provides the tools for practical method development and computer-aided optimization of separations starting from a minimum number of trial experiments.
The Effect Of Flow Rate On High Performance Liquid Chromatography Column Re Equilibration After Gradient Elution
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Author : Michael Robert Fletcher
language : en
Publisher:
Release Date : 2016
The Effect Of Flow Rate On High Performance Liquid Chromatography Column Re Equilibration After Gradient Elution written by Michael Robert Fletcher and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Chemistry categories.
In traditional high-performance liquid chromatography (HPLC), column re-equilibration between gradient elution runs is necessary to prepare a column for subsequent experiments. This process replaces the final mobile phase in the column with the mobile phase employed at the start of the run. The replacement of mobile phase occurs in four regions within the column: (i) between the particles (inter-particle); (ii) within the pores of the particles (intra-particle); (iii) in the interfacial region between the mobile phase and stationary phase; and (iv) in the space between the bonded phase ligands. Column re-equilibration, which we hypothesize is limited by the rate of diffusion within the pores and in the interfacial region, traditionally consumes a large quantity of mobile phase due to the high flow rates commonly employed during the reequilibration process. An alternative approach to column re-equilibration after gradient elution is presented, in which solvent consumption is minimized by substantially reducing the flow rate during column re-equilibration, thus allowing more time for new mobile phase to diffuse into the pores and interfacial region while consuming less solvent. The effect of flow rate on reequilibration time and solvent consumption is studied using a variety of column sizes and stationary phase composition. In addition, the effect of the solvent identity (acetonitrile vs. methanol) and the magnitude of the change in mobile phase composition on the re-equilibration time are also evaluated. The volume of mobile phase needed for re-equilibration is shown to be proportional to the flow rate employed during the re-equilibration process, verifying that a reduction in solvent consumption can be achieved by lowering the flow rate. In addition, the time necessary for re-equilibration is shown to be influenced by two terms: one that is flow rate dependent, and the other that is flow rate independent.
Liquid Column Chromatography
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Author : K. Macek
language : en
Publisher: Elsevier
Release Date : 2011-09-21
Liquid Column Chromatography written by K. Macek and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-21 with Science categories.
Liquid Column Chromatography
Introduction To Modern Liquid Chromatography
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Author : Lloyd R. Snyder
language : en
Publisher: John Wiley & Sons
Release Date : 2011-09-20
Introduction To Modern Liquid Chromatography written by Lloyd R. Snyder and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-20 with Science categories.
The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.
Gradient Hplc For Practitioners
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Author : Stavros Kromidas
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-16
Gradient Hplc For Practitioners written by Stavros Kromidas and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-16 with Science categories.
This practical guide for analytical scientists explains the use of gradients in liquid chromatography. The fundamentals of gradient separations, as well as the most common application scenarios are addressed, from LC-MS coupling to biochromatography to the separation of ionic substances. Throughout, this handy volume provides detailed hands-on information for practitioners, enabling them to use gradient separation methods reliably and efficiently.
Quantitative Column Liquid Chromatography
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Author : S.T. Balke
language : en
Publisher: Elsevier
Release Date : 2000-04-01
Quantitative Column Liquid Chromatography written by S.T. Balke and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-04-01 with Science categories.
Quantitative Column Liquid Chromatography
High Performance Liquid Chromatography
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Author : Mary T. Gilbert
language : en
Publisher: Elsevier
Release Date : 2013-10-22
High Performance Liquid Chromatography written by Mary T. Gilbert and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Science categories.
High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.